FEATURES             Location/Qualifiers
     rep_origin      1821..2733
                     /label="P15A\ori
     CDS             complement(2857..3939)
                     /label="lacI
     promoter        4063..4079
                     /label="T7\Promoter
     protein_bind    5..25
                     /label="lacO
     CDS             complement(800..1459)
                     /label="CAT
     RBS             58..63
                     /label="RBS-1
     Region          83..100
                     /label="His-Tag
     terminator      533..580
                     /label="T7\Terminator
     Region          437..481
                     /label="S-Tag
     protein_bind    235..255
                     /label="lacO
     promoter        214..230
                     /label="T7\promoter
     RBS             286..291
                     /label="RBS
     primer          4020..4039
                     /label="ACYCDuetUP1\Primer\#71178-3
     primer          189..208
                     /label="DuetUP2\Primer\#71180-3
     primer          complement(189..208)
                     /label="DuetDOWN1\Primer\#71179-3
     primer          complement(519..537)
                     /label="T7\Terminator\Primer\#69337-3
     misc_feature    297..430
                     /note="pMCSG7"
     CDS             300..383
                     /note="coding region"
     Region          303..320
                     /note="His Tag"
     Cleavage-site   345..365
                     /note="TEV Site"
     misc_feature    353..384
                     /note="LIC Site"

         1 GGGGAATTGT GAGCGGATAA CAATTCCCCT GTAGAAATAA TTTTGTTTAA CTTTAATAAG
        61 GAGATATACC ATGGGCAGCA GCCATCACCA TCATCACCAC AGCCAGGATC CGAATTCGAG
       121 CTCGGCGCGC CTGCAGGTCG ACAAGCTTGC GGCCGCATAA TGCTTAAGTC GAACAGAAAG
       181 TAATCGTATT GTACACGGCC GCATAATCGA AATTAATACG ACTCACTATA GGGGAATTGT
       241 GAGCGGATAA CAATTCCCCA TCTTAGTATA TTAGTTAAGT ATAAGAAGGA GATATACATA
       301 TGCACCATCA TCATCATCAT TCTTCTGGTG TAGATCTGGG TACCGAGAAC CTGTACTTCC
       361 AATCCAATAT TGGAAGTGGA TAACGGATCC GAATTCGAGC TCCGTCGACA AGCTTGCGGC
       421 CGCACTCGAG TCTGGTAAAG AAACCGCTGC TGCGAAATTT GAACGCCAGC ACATGGACTC
       481 GTCTACTAGC GCAGCTTAAT TAACCTAGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA
       541 ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAACCTC AGGCATTTGA
       601 GAAGCACACG GTCACACTGC TTCCGGTAGT CAATAAACCG GTAAACCAGC AATAGACATA
       661 AGCGGCTATT TAACGACCCT GCCCTGAACC GACGACCGGG TCGAATTTGC TTTCGAATTT
       721 CTGCCATTCA TCCGCTTATT ATCACTTATT CAGGCGTAGC ACCAGGCGTT TAAGGGCACC
       781 AATAACTGCC TTAAAAAAAT TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT
       841 CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAGACGGC ATGATGAACC TGAATCGCCA
       901 GCGGCATCAG CACCTTGTCG CCTTGCGTAT AGTATTTGCC CATAGTGAAA ACGGGGGCGA
       961 AGAAGTTGTC CATATTGGCC ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG
      1021 CTGAGACGAA AAACATATTC TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT
      1081 AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC
      1141 TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC
      1201 TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG GAACTCCGGA TGAGCATTCA
      1261 TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG
      1321 TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG
      1381 ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC
      1441 CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAT AACTCAAAAA
      1501 ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT GGAACCTCTT ACGTGCCGAT
      1561 CAACGTCTCA TTTTCGCCAA AAGTTGGCCC AGGGCTTCCC GGTATCAACA GGGACACCAG
      1621 GATTTATTTA TTCTGCGAAG TGATCTTCCG TCACAGGTAT TTATTCGGCG CAAAGTGCGT
      1681 CGGGTGATGC TGCCAACTTA CTGATTTAGT GTATGATGGT GTTTTTGAGG TGCTCCAGTG
      1741 GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG ACGGGGTGGT GCGTAACGGC
      1801 AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT GGCTTACTAT GTTGGCACTG
      1861 ATGAGGGTGT CAGTGAAGTG CTTCATGTGG CAGGAGAAAA AAGGCTGCAC CGGTGCGTCA
      1921 GCAGAATATG TGATACAGGA TATATTCCGC TTCCTCGCTC ACTGACTCGC TACGCTCGGT
      1981 CGTTCGACTG CGGCGAGCGG AAATGGCTTA CGAACGGGGC GGAGATTTCC TGGAAGATGC
      2041 CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA AGCCGTTTTT CCATAGGCTC
      2101 CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC AGTGGTGGCG AAACCCGACA
      2161 GGACTATAAA GATACCAGGC GTTTCCCCTG GCGGCTCCCT CGTGCGCTCT CCTGTTCCTG
      2221 CCTTTCGGTT TACCGGTGTC ATTCCGCTGT TATGGCCGCG TTTGTCTCAT TCCACGCCTG
      2281 ACACTCAGTT CCGGGTAGGC AGTTCGCTCC AAGCTGGACT GTATGCACGA ACCCCCCGTT
      2341 CAGTCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGAAAGACAT
      2401 GCAAAAGCAC CACTGGCAGC AGCCACTGGT AATTGATTTA GAGGAGTTAG TCTTGAAGTC
      2461 ATGCGCCGGT TAAGGCTAAA CTGAAAGGAC AAGTTTTGGT GACTGCGCTC CTCCAAGCCA
      2521 GTTACCTCGG TTCAAAGAGT TGGTAGCTCA GAGAACCTTC GAAAAACCGC CCTGCAAGGC
      2581 GGTTTTTTCG TTTTCAGAGC AAGAGATTAC GCGCAGACCA AAACGATCTC AAGAAGATCA
      2641 TCTTATTAAT CAGATAAATT ATTTCTAGAT TTCAGTGCAA TTTATCTCTT CAAATGTAGC
      2701 ACCTGAAGTC AGCCCCATAC GATATAAGTT GTAATTCTCA TGTTAGTCAT GCCCCGCGCC
      2761 CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGAGA TCCCGGTGCC
      2821 TAATGAGTGA GCTAACTTAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
      2881 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT
      2941 ATTGGGCGCC AGGGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT GATTGCCCTT
      3001 CACCGCCTGG CCCTGAGAGA GTTGCAGCAA GCGGTCCACG CTGGTTTGCC CCAGCAGGCG
      3061 AAAATCCTGT TTGATGGTGG TTAACGGCGG GATATAACAT GAGCTGTCTT CGGTATCGTC
      3121 GTATCCCACT ACCGAGATGT CCGCACCAAC GCGCAGCCCG GACTCGGTAA TGGCGCGCAT
      3181 TGCGCCCAGC GCCATCTGAT CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT
      3241 CAGCATTTGC ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC
      3301 TATCGGCTGA ATTTGATTGC GAGTGAGATA TTTATGCCAG CCAGCCAGAC GCAGACGCGC
      3361 CGAGACAGAA CTTAATGGGC CCGCTAACAG CGCGATTTGC TGGTGACCCA ATGCGACCAG
      3421 ATGCTCCACG CCCAGTCGCG TACCGTCTTC ATGGGAGAAA ATAATACTGT TGATGGGTGT
      3481 CTGGTCAGAG ACATCAAGAA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT
      3541 GGCATCCTGG TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG
      3601 ATTGTGCACC GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG ACACCACCAC
      3661 GCTGGCACCC AGTTGATCGG CGCGAGATTT AATCGCCGCG ACAATTTGCG ACGGCGCGTG
      3721 CAGGGCCAGA CTGGAGGTGG CAACGCCAAT CAGCAACGAC TGTTTGCCCG CCAGTTGTTG
      3781 TGCCACGCGG TTGGGAATGT AATTCAGCTC CGCCATCGCC GCTTCCACTT TTTCCCGCGT
      3841 TTTCGCAGAA ACGTGGCTGG CCTGGTTCAC CACGCGGGAA ACGGTCTGAT AAGAGACACC
      3901 GGCATACTCT GCGACATCGT ATAACGTTAC TGGTTTCACA TTCACCACCC TGAATTGACT
      3961 CTCTTCCGGG CGCTATCATG CCATACCGCG AAAGGTTTTG CGCCATTCGA TGGTGTCCGG
      4021 GATCTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA ATTAATACGA CTCACTATA
//