FEATURES             Location/Qualifiers
     Region          339..392
                     /note="S loop;S loop"
     rep_origin      1875..2787
                     /label="P15A\ori
     CDS             complement(2911..3993)
                     /label="lacI
     promoter        4117..4133
                     /label="T7\Promoter
     protein_bind    5..25
                     /label="lacO
     CDS             complement(854..1513)
                     /label="CAT
     RBS             58..63
                     /label="RBS-1
     Region          83..100
                     /label="His-Tag
     terminator      587..634
                     /label="T7\Terminator
     Region          491..535
                     /label="S-Tag
     protein_bind    235..255
                     /label="lacO
     promoter        214..230
                     /label="T7\promoter
     RBS             286..291
                     /label="RBS
     primer          4074..4093
                     /label="ACYCDuetUP1\Primer\#71178-3
     primer          189..208
                     /label="DuetUP2\Primer\#71180-3
     primer          complement(189..208)
                     /label="DuetDOWN1\Primer\#71179-3
     primer          complement(573..591)
                     /label="T7\Terminator\Primer\#69337-3
     CDS             300..437
                     /note="coding region"
     Region          303..320
                     /note="His Tag"
     Cleavage-site   399..419
                     /note="TEV Site"
     misc_feature    407..438
                     /note="LIC Site"
     misc_feature    297..484
                     /note="pMCSG8"

         1 GGGGAATTGT GAGCGGATAA CAATTCCCCT GTAGAAATAA TTTTGTTTAA CTTTAATAAG
        61 GAGATATACC ATGGGCAGCA GCCATCACCA TCATCACCAC AGCCAGGATC CGAATTCGAG
       121 CTCGGCGCGC CTGCAGGTCG ACAAGCTTGC GGCCGCATAA TGCTTAAGTC GAACAGAAAG
       181 TAATCGTATT GTACACGGCC GCATAATCGA AATTAATACG ACTCACTATA GGGGAATTGT
       241 GAGCGGATAA CAATTCCCCA TCTTAGTATA TTAGTTAAGT ATAAGAAGGA GATATACATA
       301 TGCACCATCA TCATCATCAT TCTTCTGGTG TAGATCTGGA AGTTGAAACC AAATCTGCTG
       361 GTGGTATCGT TCTGACCGGT TCTGCTGCTG CTGGTACCGA GAACCTGTAC TTCCAATCCA
       421 ATATTGGAAG TGGATAACGG ATCCGAATTC GAGCTCCGTC GACAAGCTTG CGGCCGCACT
       481 CGAGTCTGGT AAAGAAACCG CTGCTGCGAA ATTTGAACGC CAGCACATGG ACTCGTCTAC
       541 TAGCGCAGCT TAATTAACCT AGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT
       601 TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA CCTCAGGCAT TTGAGAAGCA
       661 CACGGTCACA CTGCTTCCGG TAGTCAATAA ACCGGTAAAC CAGCAATAGA CATAAGCGGC
       721 TATTTAACGA CCCTGCCCTG AACCGACGAC CGGGTCGAAT TTGCTTTCGA ATTTCTGCCA
       781 TTCATCCGCT TATTATCACT TATTCAGGCG TAGCACCAGG CGTTTAAGGG CACCAATAAC
       841 TGCCTTAAAA AAATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA
       901 GCATTCTGCC GACATGGAAG CCATCACAGA CGGCATGATG AACCTGAATC GCCAGCGGCA
       961 TCAGCACCTT GTCGCCTTGC GTATAGTATT TGCCCATAGT GAAAACGGGG GCGAAGAAGT
      1021 TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA
      1081 CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG
      1141 CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA
      1201 GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC
      1261 ATATCACCAG CTCACCGTCT TTCATTGCCA TACGGAACTC CGGATGAGCA TTCATCAGGC
      1321 GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA
      1381 AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA
      1441 ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA
      1501 TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGATAACTCA AAAAATACGC
      1561 CCGGTAGTGA TCTTATTTCA TTATGGTGAA AGTTGGAACC TCTTACGTGC CGATCAACGT
      1621 CTCATTTTCG CCAAAAGTTG GCCCAGGGCT TCCCGGTATC AACAGGGACA CCAGGATTTA
      1681 TTTATTCTGC GAAGTGATCT TCCGTCACAG GTATTTATTC GGCGCAAAGT GCGTCGGGTG
      1741 ATGCTGCCAA CTTACTGATT TAGTGTATGA TGGTGTTTTT GAGGTGCTCC AGTGGCTTCT
      1801 GTTTCTATCA GCTGTCCCTC CTGTTCAGCT ACTGACGGGG TGGTGCGTAA CGGCAAAAGC
      1861 ACCGCCGGAC ATCAGCGCTA GCGGAGTGTA TACTGGCTTA CTATGTTGGC ACTGATGAGG
      1921 GTGTCAGTGA AGTGCTTCAT GTGGCAGGAG AAAAAAGGCT GCACCGGTGC GTCAGCAGAA
      1981 TATGTGATAC AGGATATATT CCGCTTCCTC GCTCACTGAC TCGCTACGCT CGGTCGTTCG
      2041 ACTGCGGCGA GCGGAAATGG CTTACGAACG GGGCGGAGAT TTCCTGGAAG ATGCCAGGAA
      2101 GATACTTAAC AGGGAAGTGA GAGGGCCGCG GCAAAGCCGT TTTTCCATAG GCTCCGCCCC
      2161 CCTGACAAGC ATCACGAAAT CTGACGCTCA AATCAGTGGT GGCGAAACCC GACAGGACTA
      2221 TAAAGATACC AGGCGTTTCC CCTGGCGGCT CCCTCGTGCG CTCTCCTGTT CCTGCCTTTC
      2281 GGTTTACCGG TGTCATTCCG CTGTTATGGC CGCGTTTGTC TCATTCCACG CCTGACACTC
      2341 AGTTCCGGGT AGGCAGTTCG CTCCAAGCTG GACTGTATGC ACGAACCCCC CGTTCAGTCC
      2401 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGAAAG ACATGCAAAA
      2461 GCACCACTGG CAGCAGCCAC TGGTAATTGA TTTAGAGGAG TTAGTCTTGA AGTCATGCGC
      2521 CGGTTAAGGC TAAACTGAAA GGACAAGTTT TGGTGACTGC GCTCCTCCAA GCCAGTTACC
      2581 TCGGTTCAAA GAGTTGGTAG CTCAGAGAAC CTTCGAAAAA CCGCCCTGCA AGGCGGTTTT
      2641 TTCGTTTTCA GAGCAAGAGA TTACGCGCAG ACCAAAACGA TCTCAAGAAG ATCATCTTAT
      2701 TAATCAGATA AATTATTTCT AGATTTCAGT GCAATTTATC TCTTCAAATG TAGCACCTGA
      2761 AGTCAGCCCC ATACGATATA AGTTGTAATT CTCATGTTAG TCATGCCCCG CGCCCACCGG
      2821 AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GAGATCCCGG TGCCTAATGA
      2881 GTGAGCTAAC TTACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG
      2941 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
      3001 CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC
      3061 CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC
      3121 CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC
      3181 CACTACCGAG ATGTCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC
      3241 CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT
      3301 TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG
      3361 CTGAATTTGA TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC
      3421 AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC
      3481 CACGCCCAGT CGCGTACCGT CTTCATGGGA GAAAATAATA CTGTTGATGG GTGTCTGGTC
      3541 AGAGACATCA AGAAATAACG CCGGAACATT AGTGCAGGCA GCTTCCACAG CAATGGCATC
      3601 CTGGTCATCC AGCGGATAGT TAATGATCAG CCCACTGACG CGTTGCGCGA GAAGATTGTG
      3661 CACCGCCGCT TTACAGGCTT CGACGCCGCT TCGTTCTACC ATCGACACCA CCACGCTGGC
      3721 ACCCAGTTGA TCGGCGCGAG ATTTAATCGC CGCGACAATT TGCGACGGCG CGTGCAGGGC
      3781 CAGACTGGAG GTGGCAACGC CAATCAGCAA CGACTGTTTG CCCGCCAGTT GTTGTGCCAC
      3841 GCGGTTGGGA ATGTAATTCA GCTCCGCCAT CGCCGCTTCC ACTTTTTCCC GCGTTTTCGC
      3901 AGAAACGTGG CTGGCCTGGT TCACCACGCG GGAAACGGTC TGATAAGAGA CACCGGCATA
      3961 CTCTGCGACA TCGTATAACG TTACTGGTTT CACATTCACC ACCCTGAATT GACTCTCTTC
      4021 CGGGCGCTAT CATGCCATAC CGCGAAAGGT TTTGCGCCAT TCGATGGTGT CCGGGATCTC
      4081 GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAATTAAT ACGACTCACT ATA
//