FEATURES             Location/Qualifiers
     Region          345..1442
                     /note="MBP;MBP"
     rep_origin      2940..3852
                     /label="P15A\ori
     CDS             complement(3976..5058)
                     /label="lacI
     promoter        5182..5198
                     /label="T7\Promoter
     protein_bind    5..25
                     /label="lacO
     CDS             complement(1919..2578)
                     /label="CAT
     RBS             58..63
                     /label="RBS-1
     Region          83..100
                     /label="His-Tag
     terminator      1652..1699
                     /label="T7\Terminator
     Region          1556..1600
                     /label="S-Tag
     protein_bind    235..255
                     /label="lacO
     promoter        214..230
                     /label="T7\promoter
     RBS             286..291
                     /label="RBS
     primer          5139..5158
                     /label="ACYCDuetUP1\Primer\#71178-3
     primer          189..208
                     /label="DuetUP2\Primer\#71180-3
     primer          complement(189..208)
                     /label="DuetDOWN1\Primer\#71179-3
     primer          complement(1638..1656)
                     /label="T7\Terminator\Primer\#69337-3
     CDS             300..1502
                     /note="coding region"
     Region          303..320
                     /note="His Tag"
     Cleavage-site   1464..1484
                     /note="TEV Site"
     misc_feature    1472..1503
                     /note="LIC Site"
     misc_feature    297..1549
                     /note="pMCSG9"

         1 GGGGAATTGT GAGCGGATAA CAATTCCCCT GTAGAAATAA TTTTGTTTAA CTTTAATAAG
        61 GAGATATACC ATGGGCAGCA GCCATCACCA TCATCACCAC AGCCAGGATC CGAATTCGAG
       121 CTCGGCGCGC CTGCAGGTCG ACAAGCTTGC GGCCGCATAA TGCTTAAGTC GAACAGAAAG
       181 TAATCGTATT GTACACGGCC GCATAATCGA AATTAATACG ACTCACTATA GGGGAATTGT
       241 GAGCGGATAA CAATTCCCCA TCTTAGTATA TTAGTTAAGT ATAAGAAGGA GATATACATA
       301 TGCACCATCA TCATCATCAT TCTTCTGGTG TAGATCTGGG TACCAAAATC GAAGAAGGTA
       361 AACTGGTAAT CTGGATTAAC GGCGATAAAG GCTATAACGG TCTCGCTGAA GTCGGTAAGA
       421 AATTCGAGAA AGATACCGGA ATTAAAGTCA CCGTTGAGCA TCCGGATAAA CTGGAAGAGA
       481 AATTCCCACA GGTTGCGGCA ACTGGCGATG GCCCTGACAT TATCTTCTGG GCACACGACC
       541 GCTTTGGTGG CTACGCTCAA TCTGGCCTGT TGGCTGAAAT CACCCCGGAC AAAGCGTTCC
       601 AGGACAAGCT GTATCCGTTT ACCTGGGATG CCGTACGTTA CAACGGCAAG CTGATTGCTT
       661 ACCCGATCGC TGTTGAAGCG TTATCGCTGA TTTATAACAA AGATCTGCTG CCGAACCCGC
       721 CAAAAACCTG GGAAGAGATC CCGGCGCTGG ATAAAGAACT GAAAGCGAAA GGTAAGAGCG
       781 CGCTGATGTT CAACCTGCAA GAACCGTACT TCACCTGGCC GCTGATTGCT GCTGACGGGG
       841 GTTATGCGTT CAAGTATGAA AACGGCAAGT ACGACATTAA AGACGTGGGC GTGGATAACG
       901 CTGGCGCGAA AGCGGGTCTG ACCTTCCTGG TTGACCTGAT TAAAAACAAA CACATGAATG
       961 CAGACACCGA TTACTCCATC GCAGAAGCTG CCTTTAATAA AGGCGAAACA GCGATGACCA
      1021 TCAACGGCCC GTGGGCATGG TCCAACATCG ACACCAGCAA AGTGAATTAT GGTGTAACGG
      1081 TACTGCCGAC CTTCAAGGGT CAACCATCCA AACCGTTCGT TGGCGTGCTG AGCGCAGGTA
      1141 TTAACGCCGC CAGTCCGAAC AAAGAGCTGG CAAAAGAGTT CCTCGAAAAC TATCTGCTGA
      1201 CTGATGAAGG TCTGGAAGCG GTTAATAAAG ACAAACCGCT GGGTGCCGTA GCGCTGAAGT
      1261 CTTACGAGGA AGAGTTGGCG AAAGATCCAC GTATTGCCGC CACCATGGAA AACGCCCAGA
      1321 AAGGTGAAAT CATGCCGAAC ATCCCGCAGA TGTCCGCTTT CTGGTATGCC GTGCGTACTG
      1381 CGGTGATCAA CGCCGCCAGC GGTCGTCAGA CTGTCGATGA AGCCCTGAAA GACGCGCAGA
      1441 CTGATTACGA TATCCCAGGT ACCGAGAACC TGTACTTCCA ATCCAATATT GGAAGTGGAT
      1501 AACGGATCCG AATTCGAGCT CCGTCGACAA GCTTGCGGCC GCACTCGAGT CTGGTAAAGA
      1561 AACCGCTGCT GCGAAATTTG AACGCCAGCA CATGGACTCG TCTACTAGCG CAGCTTAATT
      1621 AACCTAGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG
      1681 GGTCTTGAGG GGTTTTTTGC TGAAACCTCA GGCATTTGAG AAGCACACGG TCACACTGCT
      1741 TCCGGTAGTC AATAAACCGG TAAACCAGCA ATAGACATAA GCGGCTATTT AACGACCCTG
      1801 CCCTGAACCG ACGACCGGGT CGAATTTGCT TTCGAATTTC TGCCATTCAT CCGCTTATTA
      1861 TCACTTATTC AGGCGTAGCA CCAGGCGTTT AAGGGCACCA ATAACTGCCT TAAAAAAATT
      1921 ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT
      1981 GGAAGCCATC ACAGACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC
      2041 CTTGCGTATA GTATTTGCCC ATAGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA
      2101 CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT
      2161 CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT
      2221 ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT
      2281 CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC
      2341 CGTCTTTCAT TGCCATACGG AACTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA
      2401 TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT
      2461 CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT
      2521 CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATTT
      2581 TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT AGTGATCTTA
      2641 TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCGATC AACGTCTCAT TTTCGCCAAA
      2701 AGTTGGCCCA GGGCTTCCCG GTATCAACAG GGACACCAGG ATTTATTTAT TCTGCGAAGT
      2761 GATCTTCCGT CACAGGTATT TATTCGGCGC AAAGTGCGTC GGGTGATGCT GCCAACTTAC
      2821 TGATTTAGTG TATGATGGTG TTTTTGAGGT GCTCCAGTGG CTTCTGTTTC TATCAGCTGT
      2881 CCCTCCTGTT CAGCTACTGA CGGGGTGGTG CGTAACGGCA AAAGCACCGC CGGACATCAG
      2941 CGCTAGCGGA GTGTATACTG GCTTACTATG TTGGCACTGA TGAGGGTGTC AGTGAAGTGC
      3001 TTCATGTGGC AGGAGAAAAA AGGCTGCACC GGTGCGTCAG CAGAATATGT GATACAGGAT
      3061 ATATTCCGCT TCCTCGCTCA CTGACTCGCT ACGCTCGGTC GTTCGACTGC GGCGAGCGGA
      3121 AATGGCTTAC GAACGGGGCG GAGATTTCCT GGAAGATGCC AGGAAGATAC TTAACAGGGA
      3181 AGTGAGAGGG CCGCGGCAAA GCCGTTTTTC CATAGGCTCC GCCCCCCTGA CAAGCATCAC
      3241 GAAATCTGAC GCTCAAATCA GTGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
      3301 TTTCCCCTGG CGGCTCCCTC GTGCGCTCTC CTGTTCCTGC CTTTCGGTTT ACCGGTGTCA
      3361 TTCCGCTGTT ATGGCCGCGT TTGTCTCATT CCACGCCTGA CACTCAGTTC CGGGTAGGCA
      3421 GTTCGCTCCA AGCTGGACTG TATGCACGAA CCCCCCGTTC AGTCCGACCG CTGCGCCTTA
      3481 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GAAAGACATG CAAAAGCACC ACTGGCAGCA
      3541 GCCACTGGTA ATTGATTTAG AGGAGTTAGT CTTGAAGTCA TGCGCCGGTT AAGGCTAAAC
      3601 TGAAAGGACA AGTTTTGGTG ACTGCGCTCC TCCAAGCCAG TTACCTCGGT TCAAAGAGTT
      3661 GGTAGCTCAG AGAACCTTCG AAAAACCGCC CTGCAAGGCG GTTTTTTCGT TTTCAGAGCA
      3721 AGAGATTACG CGCAGACCAA AACGATCTCA AGAAGATCAT CTTATTAATC AGATAAATTA
      3781 TTTCTAGATT TCAGTGCAAT TTATCTCTTC AAATGTAGCA CCTGAAGTCA GCCCCATACG
      3841 ATATAAGTTG TAATTCTCAT GTTAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG
      3901 TTGAAGGCTC TCAAGGGCAT CGGTCGAGAT CCCGGTGCCT AATGAGTGAG CTAACTTACA
      3961 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT
      4021 TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA GGGTGGTTTT
      4081 TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC CCTGAGAGAG
      4141 TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT TGATGGTGGT
      4201 TAACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA CCGAGATGTC
      4261 CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG CCATCTGATC
      4321 GTTGGCAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC AGCATTTGCA TGGTTTGTTG
      4381 AAAACCGGAC ATGGCACTCC AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA TTTGATTGCG
      4441 AGTGAGATAT TTATGCCAGC CAGCCAGACG CAGACGCGCC GAGACAGAAC TTAATGGGCC
      4501 CGCTAACAGC GCGATTTGCT GGTGACCCAA TGCGACCAGA TGCTCCACGC CCAGTCGCGT
      4561 ACCGTCTTCA TGGGAGAAAA TAATACTGTT GATGGGTGTC TGGTCAGAGA CATCAAGAAA
      4621 TAACGCCGGA ACATTAGTGC AGGCAGCTTC CACAGCAATG GCATCCTGGT CATCCAGCGG
      4681 ATAGTTAATG ATCAGCCCAC TGACGCGTTG CGCGAGAAGA TTGTGCACCG CCGCTTTACA
      4741 GGCTTCGACG CCGCTTCGTT CTACCATCGA CACCACCACG CTGGCACCCA GTTGATCGGC
      4801 GCGAGATTTA ATCGCCGCGA CAATTTGCGA CGGCGCGTGC AGGGCCAGAC TGGAGGTGGC
      4861 AACGCCAATC AGCAACGACT GTTTGCCCGC CAGTTGTTGT GCCACGCGGT TGGGAATGTA
      4921 ATTCAGCTCC GCCATCGCCG CTTCCACTTT TTCCCGCGTT TTCGCAGAAA CGTGGCTGGC
      4981 CTGGTTCACC ACGCGGGAAA CGGTCTGATA AGAGACACCG GCATACTCTG CGACATCGTA
      5041 TAACGTTACT GGTTTCACAT TCACCACCCT GAATTGACTC TCTTCCGGGC GCTATCATGC
      5101 CATACCGCGA AAGGTTTTGC GCCATTCGAT GGTGTCCGGG ATCTCGACGC TCTCCCTTAT
      5161 GCGACTCCTG CATTAGGAAA TTAATACGAC TCACTATA
//