FEATURES             Location/Qualifiers
     CDS             complement(208..927)
                     /note="GFP cds"
     misc_feature    complement(927..958)
                     /note="LIC"
     Cleavage-site   complement(946..966)
                     /note="TEV"
     Region          complement(991..1008)
                     /note="His tag"
     CDS             complement(4761..5621)
                     /note="beta lactamase"
     CDS             complement(208..1011)
                     /note="coding region"
     promoter        complement(1083..1099)
                     /note="T7 Promoter"
     Region          complement(140..157)
                     /note="His Tag;carboxy terminal His tag from pET-21a"
     terminator      complement(26..72)
                     /note="T7 Terminator"
     CDS             1613..2572
                     /note="Lac I cds"
     rep_origin      4003..4003
                     /note="pBR322 origin of replication"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTATCA GTTGTACAGT TCATCCATGC CATGTGTAAT
       241 CCCAGCAGCT GTTACAAACT CAAGAAGGAC CATGTGGTCT CTCTTTTCGT TGGGATCTTT
       301 CGAAAGGGCA GATTGTGTGG ACAGGTAATG GTTGTCTGGT AAAAGGACAG GGCCATCGCC
       361 AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG CTTCCATCTT CAATGTTGTG
       421 GCGGGTCTTG AAGTTCACTT TGATTCCATT CTTTTGTTTG TCTGCCATGA TGTATACATT
       481 GTGTGAGTTA TAGTTGTATT CCAATTTGTG TCCCAGAATG TTGCCATCTT CCTTGAAGTC
       541 AATACCTTTT AACTCGATTC TATTAACAAG GGTATCACCT TCAAACTTGA CTTCAGCACG
       601 TGTCTTGTAG TTGCCGTCAT CTTTGAAGAA GATGGTCCTT TCCTGTACAT AACCTTCGGG
       661 CATGGCACTC TTGAAAAAGT CATGCCGTTT CATATGATCC GGGTATCTTG AAAAGCATTG
       721 AACACCATAG CACAGAGTAG TGACTAGTGT TGGCCATGGA ACAGGCAGTT TGCCAGTAGT
       781 GCAGATGAAC TTCAGGGTAA GTTTTCCGTA TGTTGCATCA CCTTCACCCT CTCCACTGAC
       841 AGAGAACTTG TGGCCGTTAA CATCACCATC TAATTCAACA AGAATTGGGA CAACTCCAGT
       901 GAAGAGTTCT TCTCCTTTGC TAGCCAGTAA TCCACTTCCA ATATTGGATT GGAAGTACAG
       961 GTTCTCGGTA CCCAGATCTA CACCAGAAGA ATGATGATGA TGATGGTGCA TATGTATATC
      1021 TCCTTCTTAA AGTTAAACAA AATTATTTCT AGAGGGGAAT TGTTATCCGC TCACAATTCC
      1081 CCTATAGTGA GTCGTATTAA TTTCGCGGGA TCGAGATCGA TCTCGATCCT CTACGCCGGA
      1141 CGCATCGTGG CCGGCATCAC CGGCGCCACA GGTGCGGTTG CTGGCGCCTA TATCGCCGAC
      1201 ATCACCGATG GGGAAGATCG GGCTCGCCAC TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG
      1261 GGTATGGTGG CAGGCCCCGT GGCCGGGGGA CTGTTGGGCG CCATCTCCTT GCATGCACCA
      1321 TTCCTTGCGG CGGCGGTGCT CAACGGCCTC AACCTACTAC TGGGCTGCTT CCTAATGCAG
      1381 GAGTCGCATA AGGGAGAGCG TCGAGATCCC GGACACCATC GAATGGCGCA AAACCTTTCG
      1441 CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG GTGGTGAATG TGAAACCAGT
      1501 AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT
      1561 GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA
      1621 GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT
      1681 TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA
      1741 ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT
      1801 CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT
      1861 TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC
      1921 GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC AGTATTATTT TCTCCCATGA
      1981 AGACGGTACG CGACTGGGCG TGGAGCATCT GGTCGCATTG GGTCACCAGC AAATCGCGCT
      2041 GTTAGCGGGC CCATTAAGTT CTGTCTCGGC GCGTCTGCGT CTGGCTGGCT GGCATAAATA
      2101 TCTCACTCGC AATCAAATTC AGCCGATAGC GGAACGGGAA GGCGACTGGA GTGCCATGTC
      2161 CGGTTTTCAA CAAACCATGC AAATGCTGAA TGAGGGCATC GTTCCCACTG CGATGCTGGT
      2221 TGCCAACGAT CAGATGGCGC TGGGCGCAAT GCGCGCCATT ACCGAGTCCG GGCTGCGCGT
      2281 TGGTGCGGAT ATCTCGGTAG TGGGATACGA CGATACCGAA GACAGCTCAT GTTATATCCC
      2341 GCCGTTAACC ACCATCAAAC AGGATTTTCG CCTGCTGGGG CAAACCAGCG TGGACCGCTT
      2401 GCTGCAACTC TCTCAGGGCC AGGCGGTGAA GGGCAATCAG CTGTTGCCCG TCTCACTGGT
      2461 GAAAAGAAAA ACCACCCTGG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
      2521 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
      2581 CAATTAATGT AAGTTAGCTC ACTCATTAGG CACCGGGATC TCGACCGATG CCCTTGAGAG
      2641 CCTTCAACCC AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT GACTATCGTC GCCGCACTTA
      2701 TGACTGTCTT CTTTATCATG CAACTCGTAG GACAGGTGCC GGCAGCGCTC TGGGTCATTT
      2761 TCGGCGAGGA CCGCTTTCGC TGGAGCGCGA CGATGATCGG CCTGTCGCTT GCGGTATTCG
      2821 GAATCTTGCA CGCCCTCGCT CAAGCCTTCG TCACTGGTCC CGCCACCAAA CGTTTCGGCG
      2881 AGAAGCAGGC CATTATCGCC GGCATGGCGG CCCCACGGGT GCGCATGATC GTGCTCCTGT
      2941 CGTTGAGGAC CCGGCTAGGC TGGCGGGGTT GCCTTACTGG TTAGCAGAAT GAATCACCGA
      3001 TACGCGAGCG AACGTGAAGC GACTGCTGCT GCAAAACGTC TGCGACCTGA GCAACAACAT
      3061 GAATGGTCTT CGGTTTCCGT GTTTCGTAAA GTCTGGAAAC GCGGAAGTCA GCGCCCTGCA
      3121 CCATTATGTT CCGGATCTGC ATCGCAGGAT GCTGCTGGCT ACCCTGTGGA ACACCTACAT
      3181 CTGTATTAAC GAAGCGCTGG CATTGACCCT GAGTGATTTT TCTCTGGTCC CGCCGCATCC
      3241 ATACCGCCAG TTGTTTACCC TCACAACGTT CCAGTAACCG GGCATGTTCA TCATCAGTAA
      3301 CCCGTATCGT GAGCATCCTC TCTCGTTTCA TCGGTATCAT TACCCCCATG AACAGAAATC
      3361 CCCCTTACAC GGAGGCATCA GTGACCAAAC AGGAAAAAAC CGCCCTTAAC ATGGCCCGCT
      3421 TTATCAGAAG CCAGACATTA ACGCTTCTGG AGAAACTCAA CGAGCTGGAC GCGGATGAAC
      3481 AGGCAGACAT CTGTGAATCG CTTCACGACC ACGCTGATGA GCTTTACCGC AGCTGCCTCG
      3541 CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG
      3601 CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG
      3661 GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA TAGCGGAGTG TATACTGGCT
      3721 TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATATGCG GTGTGAAATA
      3781 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT
      3841 GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA
      3901 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG
      3961 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC
      4021 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
      4081 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
      4141 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
      4201 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
      4261 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
      4321 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
      4381 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
      4441 AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
      4501 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
      4561 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
      4621 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
      4681 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
      4741 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
      4801 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
      4861 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT
      4921 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
      4981 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
      5041 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTGCAG GCATCGTGGT GTCACGCTCG
      5101 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
      5161 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
      5221 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
      5281 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
      5341 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
      5401 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
      5461 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
      5521 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
      5581 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAAATT
      5641 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA
      5701 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG
      5761 CGTTAATGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA
      5821 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
      5881 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
      5941 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC
      6001 CATTCGCCA
//