FEATURES             Location/Qualifiers
     misc_feature    204..235
                     /note="LIC Site"
     Cleavage-site   223..243
                     /note="TEV Site"
     Region          268..285
                     /note="His Tag"
     CDS             complement(205..1443)
                     /note="coding region"
     promoter        complement(1515..1531)
                     /note="T7 Promoter"
     Region          complement(140..157)
                     /note="His Tag;carboxy terminal His tag from pET-21a"
     terminator      complement(26..72)
                     /note="T7 Terminator"
     CDS             2045..3004
                     /note="Lac I cds"
     CDS             complement(5193..6053)
                     /note="bla cds"
     rep_origin      4405..4405
                     /note="pBR322 origin of replication"
     Region          complement(337..1443)
                     /note="maltose binding protein"
     Cleavage-site   complement(286..306)
                     /note="TVMV cleavage site"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTATCC ACTTCCAATA TTGGATTGGA AGTACAGGTT
       241 CTCGGTACCC AGATCCACGC CAGAAGAGTG ATGATGATGG TGGTGAGACT GGAAACGCAC
       301 GGTTTCCGAG CCTGCTTTTT TGTACAAACT TGTGATCGAA TTAGTCTGCG CGTCTTTCAG
       361 GGCTTCATCG ACAGTCTGAC GACCGCTGGC GGCGTTGATC ACCGCAGTAC GCACGGCATA
       421 CCAGAAAGCG GACATCTGCG GGATGTTCGG CATGATTTCA CCTTTCTGGG CGTTTTCCAT
       481 GGTGGCGGCA ATACGTGGAT CTTTCGCCAA CTCTTCCTCG TAAGACTTCA GCGCTACGGC
       541 ACCCAGCGGT TTGTCTTTAT TAACCGCTTC CAGACCTTCA TCAGTCAGCA GATAGTTTTC
       601 GAGGAACTCT TTTGCCAGCT CTTTGTTCGG ACTGGCGGCG TTAATACCTG CGCTCAGCAC
       661 GCCAACGAAC GGTTTGGATG GTTGACCCTT GAAGGTCGGC AGTACCGTTA CACCATAATT
       721 CACTTTGCTG GTGTCGATGT TGGACCATGC CCACGGGCCG TTGATGGTCA TCGCTGTTTC
       781 GCCTTTATTA AAGGCAGCTT CTGCGATGGA GTAATCGGTG TCTGCATTCA TGTGTTTGTT
       841 TTTAATCAGG TCAACCAGGA AGGTCAGACC CGCTTTCGCG CCAGCGTTAT CCACGCCCAC
       901 GTCTTTAATG TCGTACTTGC CGTTTTCATA CTTGAACGCA TAACCCCCGT CAGCAGCAAT
       961 CAGCGGCCAG GTGAAGTACG GTTCTTGCAG GTTGAACATC AGCGCGCTCT TACCTTTCGC
      1021 TTTCAGTTCT TTATCCAGCG CCGGGATCTC TTCCCAGGTT TTTGGCGGGT TCGGCAGCAG
      1081 ATCTTTGTTA TAAATCAGCG ATAACGCTTC AACAGCGATC GGGTAAGCAA TCAGCTTGCC
      1141 GTTGTAACGT ACGGCATCCC AGGTAAACGG ATACAGCTTG TCCTGGAACG CTTTGTCCGG
      1201 GGTGATTTCA GCCAACAGGC CAGATTGAGC GTAGCCACCA AAGCGGTCGT GTGCCCAGAA
      1261 GATAATGTCA GGGCCATCGC CAGTTGCCGC AACCTGTGGG AATTTCTCTT CCAGTTTATC
      1321 CGGATGCTCA ACGGTGACTT TAATTCCGGT ATCTTTCTCG AATTTCTTAC CGACTTCAGC
      1381 GAGACCGTTA TAGCCTTTAT CGCCGTTAAT CCAGATTACC AGTTTACCTT CTTCGATTTT
      1441 CATATGTATA TCTCCTTCTT AAAGTTAAAC AAAATTATTT CTAGAGGGGA ATTGTTATCC
      1501 GCTCACAATT CCCCTATAGT GAGTCGTATT AATTTCGCGG GATCGAGATC GATCTCGATC
      1561 CTCTACGCCG GACGCATCGT GGCCGGCATC ACCGGCGCCA CAGGTGCGGT TGCTGGCGCC
      1621 TATATCGCCG ACATCACCGA TGGGGAAGAT CGGGCTCGCC ACTTCGGGCT CATGAGCGCT
      1681 TGTTTCGGCG TGGGTATGGT GGCAGGCCCC GTGGCCGGGG GACTGTTGGG CGCCATCTCC
      1741 TTGCATGCAC CATTCCTTGC GGCGGCGGTG CTCAACGGCC TCAACCTACT ACTGGGCTGC
      1801 TTCCTAATGC AGGAGTCGCA TAAGGGAGAG CGTCGAGATC CCGGACACCA TCGAATGGCG
      1861 CAAAACCTTT CGCGGTATGG CATGATAGCG CCCGGAAGAG AGTCAATTCA GGGTGGTGAA
      1921 TGTGAAACCA GTAACGTTAT ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT
      1981 TTCCCGCGTG GTGAACCAGG CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC
      2041 GGCGATGGCG GAGCTGAATT ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA
      2101 GTCGTTGCTG ATTGGCGTTG CCACCTCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT
      2161 CGCGGCGATT AAATCTCGCG CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA
      2221 ACGAAGCGGC GTCGAAGCCT GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG
      2281 TGGGCTGATC ATTAACTATC CGCTGGATGA CCAGGATGCC ATTGCTGTGG AAGCTGCCTG
      2341 CACTAATGTT CCGGCGTTAT TTCTTGATGT CTCTGACCAG ACACCCATCA ACAGTATTAT
      2401 TTTCTCCCAT GAAGACGGTA CGCGACTGGG CGTGGAGCAT CTGGTCGCAT TGGGTCACCA
      2461 GCAAATCGCG CTGTTAGCGG GCCCATTAAG TTCTGTCTCG GCGCGTCTGC GTCTGGCTGG
      2521 CTGGCATAAA TATCTCACTC GCAATCAAAT TCAGCCGATA GCGGAACGGG AAGGCGACTG
      2581 GAGTGCCATG TCCGGTTTTC AACAAACCAT GCAAATGCTG AATGAGGGCA TCGTTCCCAC
      2641 TGCGATGCTG GTTGCCAACG ATCAGATGGC GCTGGGCGCA ATGCGCGCCA TTACCGAGTC
      2701 CGGGCTGCGC GTTGGTGCGG ATATCTCGGT AGTGGGATAC GACGATACCG AAGACAGCTC
      2761 ATGTTATATC CCGCCGTTAA CCACCATCAA ACAGGATTTT CGCCTGCTGG GGCAAACCAG
      2821 CGTGGACCGC TTGCTGCAAC TCTCTCAGGG CCAGGCGGTG AAGGGCAATC AGCTGTTGCC
      2881 CGTCTCACTG GTGAAAAGAA AAACCACCCT GGCGCCCAAT ACGCAAACCG CCTCTCCCCG
      2941 CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA
      3001 GTGAGCGCAA CGCAATTAAT GTAAGTTAGC TCACTCATTA GGCACCGGGA TCTCGACCGA
      3061 TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC ATGACTATCG
      3121 TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG CCGGCAGCGC
      3181 TCTGGGTCAT TTTCGGCGAG GACCGCTTTC GCTGGAGCGC GACGATGATC GGCCTGTCGC
      3241 TTGCGGTATT CGGAATCTTG CACGCCCTCG CTCAAGCCTT CGTCACTGGT CCCGCCACCA
      3301 AACGTTTCGG CGAGAAGCAG GCCATTATCG CCGGCATGGC GGCCCCACGG GTGCGCATGA
      3361 TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG GCTGGCGGGG TTGCCTTACT GGTTAGCAGA
      3421 ATGAATCACC GATACGCGAG CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT
      3481 GAGCAACAAC ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT
      3541 CAGCGCCCTG CACCATTATG TTCCGGATCT GCATCGCAGG ATGCTGCTGG CTACCCTGTG
      3601 GAACACCTAC ATCTGTATTA ACGAAGCGCT GGCATTGACC CTGAGTGATT TTTCTCTGGT
      3661 CCCGCCGCAT CCATACCGCC AGTTGTTTAC CCTCACAACG TTCCAGTAAC CGGGCATGTT
      3721 CATCATCAGT AACCCGTATC GTGAGCATCC TCTCTCGTTT CATCGGTATC ATTACCCCCA
      3781 TGAACAGAAA TCCCCCTTAC ACGGAGGCAT CAGTGACCAA ACAGGAAAAA ACCGCCCTTA
      3841 ACATGGCCCG CTTTATCAGA AGCCAGACAT TAACGCTTCT GGAGAAACTC AACGAGCTGG
      3901 ACGCGGATGA ACAGGCAGAC ATCTGTGAAT CGCTTCACGA CCACGCTGAT GAGCTTTACC
      3961 GCAGCTGCCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG
      4021 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT
      4081 CAGCGGGTGT TGGCGGGTGT CGGGGCGCAG CCATGACCCA GTCACGTAGC GATAGCGGAG
      4141 TGTATACTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATATG
      4201 CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CTCTTCCGCT
      4261 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC
      4321 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA
      4381 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT
      4441 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC
      4501 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT
      4561 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG
      4621 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG
      4681 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT
      4741 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG
      4801 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC
      4861 GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA
      4921 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT
      4981 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT
      5041 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
      5101 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
      5161 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
      5221 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
      5281 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA
      5341 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
      5401 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
      5461 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTGC AGGCATCGTG
      5521 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
      5581 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
      5641 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
      5701 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
      5761 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
      5821 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
      5881 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
      5941 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
      6001 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
      6061 CTTTTTCAAA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
      6121 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
      6181 CTAAATTGTA AGCGTTAATG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC
      6241 CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG
      6301 CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG
      6361 GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA
      6421 CAGGGCGCGT CCCATTCGCC A
//