FEATURES             Location/Qualifiers
     Cleavage-site   3251..3271
                     /note="TEV Site"
     Region          3296..3313
                     /note="His Tag"
     promoter        complement(3388..3404)
                     /note="T7 Promoter"
     terminator      complement(26..72)
                     /note="T7 Terminator"
     CDS             3918..4877
                     /note="Lac I cds"
     CDS             complement(7066..7926)
                     /note="bla cds"
     rep_origin      6308..6308
                     /note="pBR322 origin of replication"
     misc_feature    complement(2171..3247)
                     /note="MBP"
     misc_feature    complement(2153..2170)
                     /note="linker"
     misc_feature    2133..2152
                     /note="LIC2"
     misc_feature    complement(229..246)
                     /note="LIC2"
     misc_feature    complement(255..1676)
                     /note="SacB"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTATCC ACTTCCAATG TTATTTTTCA CCGACGACAA
       241 GGGCCGCCGT GGCCTTATTT GTTAACTGTT AATTGTCCTT GTTCAAGGAT GCTGTCTTTG
       301 ACAACAGATG TTTTCTTGCC TTTGATGTTC AGCAGGAAGC TTGGCGCAAA CGTTGATTGT
       361 TTGTCTGCGT AGAATCCTCT GTTTGTCATA TAGCTTGTAA TCACGACATT GTTTCCTTTC
       421 GCTTGAGGTA CAGCGAAGTG TGAGTAAGTA AAGGTTACAT CGTTAGGATC AAGATCCATT
       481 TTTAACACAA GGCCAGTTTT GTTCAGCGGC TTGTATGGGC CAGTTAAAGA ATTAGAAACA
       541 TAACCAAGCA TGTAAATATC GTTAGACGTA ATGCCGTCAA TCGTCATTTT TGATCCGCGG
       601 GAGTCAGTGA ACAGGTACCA TTTGCCGTTC ATTTTAAAGA CGTTCGCGCG TTCAATTTCA
       661 TCTGTTACTG TGTTAGATGC AATCAGCGGT TTCATCACTT TTTTCAGTGT GTAATCATCG
       721 TTTAGCTCAA TCATACCGAG AGCGCCGTTT GCTAACTCAG CCGTGCGTTT TTTATCGCTT
       781 TGCAGAAGTT TTTGACTTTC TTGACGGAAG AATGATGTGC TTTTGCCATA GTATGCTTTG
       841 TTAAATAAAG ATTCTTCGCC TTGGTAGCCA TCTTCAGTTC CAGTGTTTGC TTCAAATACT
       901 AAGTATTTGT GGCCTTTATC TTCTACGTAG TGAGGATCTC TCAGCGTATG GTTGTCGCCT
       961 GAGCTGTAGT TGCCTTCATC GATGAACTGC TGTACATTTT GATACGTTTT TCCGTCACCG
      1021 TCAAAGATTG ATTTATAATC CTCTACACCG TTGATGTTCA AAGAGCTGTC TGATGCTGAT
      1081 ACGTTAACTT GTGCAGTTGT CAGTGTTTGT TTGCCGTAAT GTTTACCGGA GAAATCAGTG
      1141 TAGAATAAAC GGATTTTTCC GTCAGATGTA AATGTGGCTG AACCTGACCA TTCTTGTGTT
      1201 TGGTCTTTTA GGATAGAATC ATTTGCATCG AATTTGTCGC TGTCTTTAAA GACGCGGCCA
      1261 GCGTTTTTCC AGCTGTCAAT AGAAGTTTCG CCGACTTTTT GATAGAACAT GTAAATCGAT
      1321 GTGTCATCCG CATTTTTAGG ATCTCCGGCT AATGCAAAGA CGATGTGGTA GCCGTGATAG
      1381 TTTGCGACAG TGCCGTCAGC GTTTTGTAAT GGCCAGCTGT CCCAAACGTC CAGGCCTTTT
      1441 GCAGAAGAGA TATTTTTAAT TGTGGACGAA TCGAATTCAG GAACTTGATA TTTTTCATTT
      1501 TTTTGCTGTT CAGGGATTTG CAGCATATCA TGGCGTGTAA TATGGGAAAT GCCGTATGTT
      1561 TCCTTATATG GCTTTTGGTT CGTTTCTTTC GCAAACGCTT GAGTTGCGCC TCCTGCCAGC
      1621 AGTGCGGTAG TAAAGGTTAA TACTGTTGCT TGTTTTGCAA ACTTTTTGAT GTTCATCGTT
      1681 CATGTCTCCT TTTTTATGTA CTGTGTTAGC GGTCTGCTTC TTCCAGCCCT CCTGTTTGAA
      1741 GATGGCAAGT TAGTTACGCA CAATAAAAAA AGACCTAAAA TATGTAAGGG GTGACGCCAA
      1801 AGTATACACT TTGCCCTTTA CACATTTTAG GTCTTGCCTG CTTTATCAGT AACAAACCCG
      1861 CGCGATTTAC TTTTCGACCT CATTCTATTA GACTCTCGTT TGGATTGCAA CTGGTCTATT
      1921 TTCCTCTTTT GTTTGATAGA AAATCATAAA AGGATTTGCA GACTACGGGC CTAAAGAACT
      1981 AAAAAATCTA TCTGTTTCTT TTCATTCTCT GTATTTTTTA TAGTTTCTGT TGCATGGGCA
      2041 TAAAGTTGCC TTTTTAATCA CAATTCAGAA AATATCATAA TATCTCATTT CACTAAATAA
      2101 TAGTGAACGG CAGGTATATG TGTTAGGCCG CCGTGGCCGC TGCGTTGGTT TGAGCAGCGG
      2161 CAAGAGCAGC ATCGACAGTC TGACGACCGC TGGCGGCGTT GATCACCGCA GTACGCACGG
      2221 CATACCAGAA AGCGGACATC TGCGGGATGT TCGGCATGAT TTCACCTTTC TGGGCGTTTT
      2281 CCATGGTGGC GGCAATACGT GGATCTTTCG CCAACTCTTC CTCGTAAGAC TTCAGCGCTA
      2341 CGGCACCCAG CGGTTTGTCT TTATTAACCG CTTCCAGACC TTCATCAGTC AGCAGATAGT
      2401 TTTCGAGGAA CTCTTTCGCC AGCTCTTTGT TCGGACTGGC GGCGTTAATA CCTGCGCTCA
      2461 GCACGCCAAC GAACGGTTTG GATGGTTGAC CCTTGAAGGT CGGCAGTACC GTTACACCAT
      2521 AATTCACTTT GCTGGTGTCG ATGTTGGACC ATGCCCACGG GCCGTTGATG GTCATCGCTG
      2581 TTTCGCCTTT ATTAAAGGCA GCTTCTGCGA TGGAGTAATC GGTGTCTGCA TTCATGTGTT
      2641 TGTTTTTAAT CAGGTCAACC AGGAAGGTCA GACCCGCTTT CGCGCCAGCG TTATCCACGC
      2701 CCACGTCTTT AATGTCGTAC TTGCCGTTTT CATACTTGAA CGCATAACCC CCGTCAGCAG
      2761 CAATCAGCGG CCAGGTGAAG TACGGTTCTT GCAGGTTGAA CATCAGCGCG CTCTTACCTT
      2821 TCGCTTTCAG TTCTTTATCC AGCGCCGGGA TCTCTTCCCA GGTTTTTGGC GGGTTCGGCA
      2881 GCAGATCTTT GTTATAAATC AGCGATAACG CTTCAACAGC GATCGGGTAA GCAATCAGCT
      2941 TGCCGTTGTA ACGTACGGCA TCCCAGGTAA ACGGATACAG CTTGTCCTGG AACGCTTTGT
      3001 CCGGGGTGAT TTCAGCCAAC AGGCCAGATT GAGCGTAGCC ACCAAAGCGG TCGTGTGCCC
      3061 AGAAGATAAT GTCAGGGCCA TCGCCAGTTG CCGCAACCTG TGGGAATTTC TCTTCCAGTT
      3121 TATCCGGATG CTCAACGGTG ACTTTAATTC CGGTATCTTT CTCGAATTTC TTACCGACTT
      3181 CAGCGAGACC GTTATAGCCT TTATCGCCGT TAATCCAGAT TACCAGTTTA CCTTCTTCGA
      3241 TTTTGGCATT GGATTGGAAG TACAGGTTCT CGGTACCCAG ATCTACACCA GAAGAATGAT
      3301 GATGATGATG GTGCATATGT ATATCTCCTT CTTAAAGTTA AACAAAATTA TTTCTAGAGG
      3361 GGAATTGTTA TCCGCTCACA ATTCCCCTAT AGTGAGTCGT ATTAATTTCG CGGGATCGAG
      3421 ATCGATCTCG ATCCTCTACG CCGGACGCAT CGTGGCCGGC ATCACCGGCG CCACAGGTGC
      3481 GGTTGCTGGC GCCTATATCG CCGACATCAC CGATGGGGAA GATCGGGCTC GCCACTTCGG
      3541 GCTCATGAGC GCTTGTTTCG GCGTGGGTAT GGTGGCAGGC CCCGTGGCCG GGGGACTGTT
      3601 GGGCGCCATC TCCTTGCATG CACCATTCCT TGCGGCGGCG GTGCTCAACG GCCTCAACCT
      3661 ACTACTGGGC TGCTTCCTAA TGCAGGAGTC GCATAAGGGA GAGCGTCGAG ATCCCGGACA
      3721 CCATCGAATG GCGCAAAACC TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT
      3781 TCAGGGTGGT GAATGTGAAA CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT
      3841 CTTATCAGAC CGTTTCCCGC GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG
      3901 AAAAAGTGGA AGCGGCGATG GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC
      3961 TGGCGGGCAA ACAGTCGTTG CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC
      4021 CGTCGCAAAT TGTCGCGGCG ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG
      4081 TGTCGATGGT AGAACGAAGC GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG
      4141 CGCAACGCGT CAGTGGGCTG ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG
      4201 TGGAAGCTGC CTGCACTAAT GTTCCGGCGT TATTTCTTGA TGTCTCTGAC CAGACACCCA
      4261 TCAACAGTAT TATTTTCTCC CATGAAGACG GTACGCGACT GGGCGTGGAG CATCTGGTCG
      4321 CATTGGGTCA CCAGCAAATC GCGCTGTTAG CGGGCCCATT AAGTTCTGTC TCGGCGCGTC
      4381 TGCGTCTGGC TGGCTGGCAT AAATATCTCA CTCGCAATCA AATTCAGCCG ATAGCGGAAC
      4441 GGGAAGGCGA CTGGAGTGCC ATGTCCGGTT TTCAACAAAC CATGCAAATG CTGAATGAGG
      4501 GCATCGTTCC CACTGCGATG CTGGTTGCCA ACGATCAGAT GGCGCTGGGC GCAATGCGCG
      4561 CCATTACCGA GTCCGGGCTG CGCGTTGGTG CGGATATCTC GGTAGTGGGA TACGACGATA
      4621 CCGAAGACAG CTCATGTTAT ATCCCGCCGT TAACCACCAT CAAACAGGAT TTTCGCCTGC
      4681 TGGGGCAAAC CAGCGTGGAC CGCTTGCTGC AACTCTCTCA GGGCCAGGCG GTGAAGGGCA
      4741 ATCAGCTGTT GCCCGTCTCA CTGGTGAAAA GAAAAACCAC CCTGGCGCCC AATACGCAAA
      4801 CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC
      4861 TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTAAGTT AGCTCACTCA TTAGGCACCG
      4921 GGATCTCGAC CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG
      4981 GGCATGACTA TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG
      5041 GTGCCGGCAG CGCTCTGGGT CATTTTCGGC GAGGACCGCT TTCGCTGGAG CGCGACGATG
      5101 ATCGGCCTGT CGCTTGCGGT ATTCGGAATC TTGCACGCCC TCGCTCAAGC CTTCGTCACT
      5161 GGTCCCGCCA CCAAACGTTT CGGCGAGAAG CAGGCCATTA TCGCCGGCAT GGCGGCCCCA
      5221 CGGGTGCGCA TGATCGTGCT CCTGTCGTTG AGGACCCGGC TAGGCTGGCG GGGTTGCCTT
      5281 ACTGGTTAGC AGAATGAATC ACCGATACGC GAGCGAACGT GAAGCGACTG CTGCTGCAAA
      5341 ACGTCTGCGA CCTGAGCAAC AACATGAATG GTCTTCGGTT TCCGTGTTTC GTAAAGTCTG
      5401 GAAACGCGGA AGTCAGCGCC CTGCACCATT ATGTTCCGGA TCTGCATCGC AGGATGCTGC
      5461 TGGCTACCCT GTGGAACACC TACATCTGTA TTAACGAAGC GCTGGCATTG ACCCTGAGTG
      5521 ATTTTTCTCT GGTCCCGCCG CATCCATACC GCCAGTTGTT TACCCTCACA ACGTTCCAGT
      5581 AACCGGGCAT GTTCATCATC AGTAACCCGT ATCGTGAGCA TCCTCTCTCG TTTCATCGGT
      5641 ATCATTACCC CCATGAACAG AAATCCCCCT TACACGGAGG CATCAGTGAC CAAACAGGAA
      5701 AAAACCGCCC TTAACATGGC CCGCTTTATC AGAAGCCAGA CATTAACGCT TCTGGAGAAA
      5761 CTCAACGAGC TGGACGCGGA TGAACAGGCA GACATCTGTG AATCGCTTCA CGACCACGCT
      5821 GATGAGCTTT ACCGCAGCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC
      5881 ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC
      5941 CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT
      6001 AGCGATAGCG GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG
      6061 TGCACCATAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG
      6121 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC
      6181 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG
      6241 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT
      6301 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
      6361 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
      6421 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
      6481 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
      6541 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
      6601 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
      6661 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
      6721 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
      6781 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
      6841 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
      6901 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
      6961 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
      7021 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
      7081 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
      7141 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
      7201 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
      7261 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
      7321 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
      7381 TGCAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
      7441 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
      7501 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
      7561 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
      7621 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
      7681 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
      7741 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
      7801 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
      7861 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
      7921 ACTCATACTC TTCCTTTTTC AAATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
      7981 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC
      8041 CGAAAAGTGC CACCTAAATT GTAAGCGTTA ATGTGAACCA TCACCCTAAT CAAGTTTTTT
      8101 GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC
      8161 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG
      8221 CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT
      8281 TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCA
//