FEATURES Location/Qualifiers CDS 184..894 /note="TVMV Protease" misc_feature 2540..2571 /note="LIC Site" Cleavage-site 2532..2552 /note="TEV Site" Region 2490..2507 /note="His Tag" promoter 1244..1260 /note="T7 Promoter" terminator 2703..2749 /note="T7 Terminator" Region 1332..2438 /note="maltose binding protein" Cleavage-site 2469..2489 /note="TVMV cleavage site" promoter 37..72 /note="Tet promoter" misc_feature 2818..2847 /note="Strep tag" CDS 3520..4380 /note="Ampicillin resistance" CDS 4390..5013 /note="Tet repressor" rep_origin 2932..3370 /note="f1 origin" 1 CCATCGAATG GCCAGATGAT TAATTCCTAA TTTTTGTTGA CACTCTATCA TTGATAGAGT 61 TATTTTACCA CTCCCTATCA GTGATAGAGA AAAGTGAAAT GAATAGTTCG ACAAAAATCT 121 AGAAATAATT TTGTTTAACT TTAAGAAGGA GATATACAAA TGGGAGACCG CGGTCCCGAA 181 TTGATGTCTA AAGCTTTGCT GAAGGGCGTG CGCGATTTTA ATCCGATCTC TGCTTGCGTA 241 TGCCTGCTGG AAAACTCCTC GGATGGTCAT AGTGAACGTC TGTTTGGCAT TGGTTTTGGC 301 CCGTATATCA TTGCCAACCA GCATCTGTTT CGTCGTAACA ATGGCGAACT GACCATCAAA 361 ACCATGCATG GTGAATTCAA AGTCAAAAAC TCTACCCAGC TGCAGATGAA ACCGGTTGAA 421 GGCCGTGACA TTATCGTTAT CAAAATGGCT AAAGACTTCC CGCCGTTCCC GCAGAAACTG 481 AAATTCCGTC AGCCGACCAT CAAAGATCGT GTGTGCATGG TGTCCACCAA CTTTCAGCAG 541 AAAAGCGTCT CGAGCCTGGT GTCTGAATCC TCTCACATTG TGCATAAAGA AGACACTTCT 601 TTCTGGCAGC ACTGGATCAC CACTAAAGAT GGCCAGTGTG GCAGCCCACT AGTTTCCATC 661 ATTGATGGCA ACATTCTGGG CATCCACAGC CTGACTCATA CCACCAACGG TAGCAACTAC 721 TTCGTGGAAT TTCCGGAAAA ATTCGTGGCG ACTTATCTAG ATGCCGCGGA TGGTTGGTGC 781 AAAAACTGGA AATTCAACGC GGATAAAATC AGCTGGGGTT CCTTTACCCT GGTTGAAGAT 841 GCGCCGGAAG ATGACTTCAT GGCCAAAAAA ACTGTTGCCG CCATCATGGA CTAATAAAGA 901 TCCCATGGTA CGCGTGCTAG AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG 961 CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGC 1021 CCTAGAGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA 1081 CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG 1141 ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG 1201 CGTCCGGCGT AGAGGATCGA GATCGATCTC GATCCCGCGA AATTAATACG ACTCACTATA 1261 GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG 1321 GAGATATACA TATGAAAATC GAAGAAGGTA AACTGGTAAT CTGGATTAAC GGCGATAAAG 1381 GCTATAACGG TCTCGCTGAA GTCGGTAAGA AATTCGAGAA AGATACCGGA ATTAAAGTCA 1441 CCGTTGAGCA TCCGGATAAA CTGGAAGAGA AATTCCCACA GGTTGCGGCA ACTGGCGATG 1501 GCCCTGACAT TATCTTCTGG GCACACGACC GCTTTGGTGG CTACGCTCAA TCTGGCCTGT 1561 TGGCTGAAAT CACCCCGGAC AAAGCGTTCC AGGACAAGCT GTATCCGTTT ACCTGGGATG 1621 CCGTACGTTA CAACGGCAAG CTGATTGCTT ACCCGATCGC TGTTGAAGCG TTATCGCTGA 1681 TTTATAACAA AGATCTGCTG CCGAACCCGC CAAAAACCTG GGAAGAGATC CCGGCGCTGG 1741 ATAAAGAACT GAAAGCGAAA GGTAAGAGCG CGCTGATGTT CAACCTGCAA GAACCGTACT 1801 TCACCTGGCC GCTGATTGCT GCTGACGGGG GTTATGCGTT CAAGTATGAA AACGGCAAGT 1861 ACGACATTAA AGACGTGGGC GTGGATAACG CTGGCGCGAA AGCGGGTCTG ACCTTCCTGG 1921 TTGACCTGAT TAAAAACAAA CACATGAATG CAGACACCGA TTACTCCATC GCAGAAGCTG 1981 CCTTTAATAA AGGCGAAACA GCGATGACCA TCAACGGCCC GTGGGCATGG TCCAACATCG 2041 ACACCAGCAA AGTGAATTAT GGTGTAACGG TACTGCCGAC CTTCAAGGGT CAACCATCCA 2101 AACCGTTCGT TGGCGTGCTG AGCGCAGGTA TTAACGCCGC CAGTCCGAAC AAAGAGCTGG 2161 CAAAAGAGTT CCTCGAAAAC TATCTGCTGA CTGATGAAGG TCTGGAAGCG GTTAATAAAG 2221 ACAAACCGCT GGGTGCCGTA GCGCTGAAGT CTTACGAGGA AGAGTTGGCG AAAGATCCAC 2281 GTATTGCCGC CACCATGGAA AACGCCCAGA AAGGTGAAAT CATGCCGAAC ATCCCGCAGA 2341 TGTCCGCTTT CTGGTATGCC GTGCGTACTG CGGTGATCAA CGCCGCCAGC GGTCGTCAGA 2401 CTGTCGATGA AGCCCTGAAA GACGCGCAGA CTAATTCGAT CACAAGTTTG TACAAAAAAG 2461 CAGGCTCGGA AACCGTGCGT TTCCAGTCTC ACCACCATCA TCATCACTCT TCTGGCGTGG 2521 ATCTGGGTAC CGAGAACCTG TACTTCCAAT CCAATATTGG AAGTGGATAA CGGATCCGAA 2581 TTCGAGCTCC GTCGACAAGC TTGCGGCCGC ACTCGAGCAC CACCACCACC ACCACTGAGA 2641 TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA 2701 ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG 2761 AACTATACCC GGGGATCCCT CGAGGTCGAC CTGCAGGGGG ACCATGGTCT CAGCGCTTGG 2821 AGCCACCCGC AGTTCGAAAA ATAATAAGCT TGACCTGTGA AGTGAAAAAT GGCGCACATT 2881 GTGCGACATT TTTTTTGTCT GCCGTTTACC GCTACTGCGT CACGGATCTC CACGCGCCCT 2941 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 3001 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 3061 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 3121 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 3181 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 3241 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 3301 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 3361 TTAACAAAAT CACATTAACG CTTACAATTT AGGTGGCACT TTTCGGGGAA ATGTGCGCGG 3421 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA 3481 ACCCTGATAA ATGCTTCAAT AATTTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 3541 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 3601 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 3661 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 3721 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 3781 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 3841 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 3901 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 3961 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 4021 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG 4081 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTGATAGAC 4141 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 4201 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGCTCTC GCGGTATCAT TGCAGCACTG 4261 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 4321 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAG 4381 GAATTAATGA TGTCTCGTTT AGATAAAAGT AAAGTGATTA ACAGCGCATT AGAGCTGCTT 4441 AATGAGGTCG GAATCGAAGG TTTAACAACC CGTAAACTCG CCCAGAAGCT AGGTGTAGAG 4501 CAGCCTACAT TGTATTGGCA TGTAAAAAAT AAGCGGGCTT TGCTCGACGC CTTAGCCATT 4561 GAGATGTTAG ATAGGCACCA TACTCACTTT TGCCCTTTAG AAGGGGAAAG CTGGCAAGAT 4621 TTTTTACGTA ATAACGCTAA AAGTTTTAGA TGTGCTTTAC TAAGTCATCG CGATGGAGCA 4681 AAAGTACATT TAGGTACACG GCCTACAGAA AAACAGTATG AAACTCTCGA AAATCAATTA 4741 GCCTTTTTAT GCCAACAAGG TTTTTCACTA GAGAATGCAT TATATGCACT CAGCGCAGTG 4801 GGGCATTTTA CTTTAGGTTG CGTATTGGAA GATCAAGAGC ATCAAGTCGC TAAAGAAGAA 4861 AGGGAAACAC CTACTACTGA TAGTATGCCG CCATTATTAC GACAAGCTAT CGAATTATTT 4921 GATCACCAAG GTGCAGAGCC AGCCTTCTTA TTCGGCCTTG AATTGATCAT ATGCGGATTA 4981 GAAAAACAAC TTAAATGTGA AAGTGGGTCT TAAAAGCAGC ATAACCTTTT TCCGTGATGG 5041 TAACTTCACT AGTTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA 5101 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA 5161 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG 5221 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT 5281 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC 5341 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG 5401 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG 5461 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA 5521 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC 5581 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG 5641 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC 5701 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC 5761 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGACCCGA 5821 CA //