FEATURES             Location/Qualifiers
     misc_feature    complement(245..265)
                     /note="LIC Site"
     Region          complement(208..225)
                     /note="His Tag"
     promoter        complement(1531..1547)
                     /note="T7 Promoter"
     Region          complement(140..157)
                     /note="His Tag;carboxy terminal His tag from pET-21a"
     terminator      complement(26..72)
                     /note="T7 Terminator"
     CDS             3088..4047
                     /note="Lac I cds"
     CDS             complement(6236..7096)
                     /note="bla cds"
     rep_origin      5478..5478
                     /note="pBR322 origin of replication"
     misc_feature    complement(232..252)
                     /note="TEV site"
     misc_feature    complement(263..283)
                     /note="TEV site"
     misc_feature    complement(305..1402)
                     /note="MBP cds"
     misc_feature    complement(1427..1456)
                     /note="His10"
     promoter        1769..1924
                     /note="TetO promoter"
     CDS             1943..2653
                     /note="TVMV protease cds"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTAATG ATGATGATGG TGGTGCCCGG CGGATTGGAA
       241 GTACAGGTTC TCCCCGGGAG AGACTTTGGA AGTACAGGTT CTCGGTACCT GGGATATCGT
       301 AATCAGTCTG CGCGTCTTTC AGGGCTTCAT CGACAGTCTG ACGACCGCTG GCGGCGTTGA
       361 TCACCGCAGT ACGCACGGCA TACCAGAAAG CGGACATCTG CGGGATGTTC GGCATGATTT
       421 CACCTTTCTG GGCGTTTTCC ATGGTGGCGG CAATACGTGG ATCTTTCGCC AACTCTTCCT
       481 CGTAAGACTT CAGCGCTACG GCACCCAGCG GTTTGTCTTT ATTAACCGCT TCCAGACCTT
       541 CATCAGTCAG CAGATAGTTT TCGAGGAACT CTTTTGCCAG CTCTTTGTTC GGACTGGCGG
       601 CGTTAATACC TGCGCTCAGC ACGCCAACGA ACGGTTTGGA TGGTTGACCC TTGAAGGTCG
       661 GCAGTACCGT TACACCATAA TTCACTTTGC TGGTGTCGAT GTTGGACCAT GCCCACGGGC
       721 CGTTGATGGT CATCGCTGTT TCGCCTTTAT TAAAGGCAGC TTCTGCGATG GAGTAATCGG
       781 TGTCTGCATT CATGTGTTTG TTTTTAATCA GGTCAACCAG GAAGGTCAGA CCCGCTTTCG
       841 CGCCAGCGTT ATCCACGCCC ACGTCTTTAA TGTCGTACTT GCCGTTTTCA TACTTGAACG
       901 CATAACCCCC GTCAGCAGCA ATCAGCGGCC AGGTGAAGTA CGGTTCTTGC AGGTTGAACA
       961 TCAGCGCGCT CTTACCTTTC GCTTTCAGTT CTTTATCCAG CGCCGGGATC TCTTCCCAGG
      1021 TTTTTGGCGG GTTCGGCAGC AGATCTTTGT TATAAATCAG CGATAACGCT TCAACAGCGA
      1081 TCGGGTAAGC AATCAGCTTG CCGTTGTAAC GTACGGCATC CCAGGTAAAC GGATACAGCT
      1141 TGTCCTGGAA CGCTTTGTCC GGGGTGATTT CAGCCAACAG GCCAGATTGA GCGTAGCCAC
      1201 CAAAGCGGTC GTGTGCCCAG AAGATAATGT CAGGGCCATC GCCAGTTGCC GCAACCTGTG
      1261 GGAATTTCTC TTCCAGTTTA TCCGGATGCT CAACGGTGAC TTTAATTCCG GTATCTTTCT
      1321 CGAATTTCTT ACCGACTTCA GCGAGACCGT TATAGCCTTT ATCGCCGTTA ATCCAGATTA
      1381 CCAGTTTACC TTCTTCGATT TTGGTACCCA GATCTACACC AGAAGAGTGG TGGTGGTGAT
      1441 GATGATGATG ATGGTGCATA TGTATATCTC CTTCTTAAAG TTAAACAAAA TTATTTCTAG
      1501 AGGGGAATTG TTATCCGCTC ACAATTCCCC TATAGTGAGT CGTATTAATT TCGCGGGATC
      1561 GAGATCGATC TCGATCCTCT ACGCCGGACG CATCGTGGCC GGCATCACCG GCGCCACAGG
      1621 TGCGGTTGCT GGCGCCTATA TCGCCGACAT CACCGATGGG GAAGATCGGG CTCGCCACTT
      1681 CGGGCTCATG AGCGCTTGTT TCGGCGTGGG TATGGTGGCA GGCCCCGTGG CCGGGGGACT
      1741 GTTGGGCGCC ATCTCCTTGC ATGCGACGTC TAAGAAACCA TTATTATCAT GACATTAACC
      1801 TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTTCACC TCGAGTCCCT ATCAGTGATA
      1861 GAGATTGACA TCCCTATCAG TGATAGAGAT ACTGAGCACA TCAGCAGGAC GCACTGACCG
      1921 AATTGATTTA AGGAGGTAAC ATATGTCTAA AGCTTTGCTG AAGGGCGTGC GCGATTTTAA
      1981 TCCGATCTCT GCTTGCGTAT GCCTGCTGGA AAACTCCTCG GATGGTCATA GTGAACGTCT
      2041 GTTTGGCATT GGTTTTGGCC CGTATATCAT TGCCAACCAG CATCTGTTTC GTCGTAACAA
      2101 TGGCGAACTG ACCATCAAAA CCATGCATGG TGAATTCAAA GTCAAAAACT CTACCCAGCT
      2161 GCAGATGAAA CCGGTTGAAG GCCGTGACAT TATCGTTATC AAAATGGCTA AAGACTTCCC
      2221 GCCGTTCCCG CAGAAACTGA AATTCCGTCA GCCGACCATC AAAGATCGTG TGTGCATGGT
      2281 GTCCACCAAC TTTCAGCAGA AAAGCGTCTC GAGCCTGGTG TCTGAATCCT CTCACATTGT
      2341 GCATAAAGAA GACACTTCTT TCTGGCAGCA CTGGATCACC ACTAAAGATG GCCAGTGTGG
      2401 CAGCCCACTA GTTTCCATCA TTGATGGCAA CATTCTGGGC ATCCACAGCC TGACTCATAC
      2461 CACCAACGGT AGCAACTACT TCGTGGAATT TCCGGAAAAA TTCGTGGCGA CTTATCTAGA
      2521 TGCCGCGGAT GGTTGGTGCA AAAACTGGAA ATTCAACGCG GATAAAATCA GCTGGGGTTC
      2581 CTTTACCCTG GTTGAAGATG CGCCGGAAGA TGACTTCATG GCCAAAAAAA CTGTTGCCGC
      2641 CATCATGGAC TAATAAAGAT CCCATGGTAC GCGTGCTAGA GGCATCAAAT AAAACGAAAG
      2701 GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG
      2761 AGTAGGACAA ATCCGCCGCC CTAGAGCATG CACCATTCCT TGCGGCGGCG GTGCTCAACG
      2821 GCCTCAACCT ACTACTGGGC TGCTTCCTAA TGCAGGAGTC GCATAAGGGA GAGCGTCGAG
      2881 ATCCCGGACA CCATCGAATG GCGCAAAACC TTTCGCGGTA TGGCATGATA GCGCCCGGAA
      2941 GAGAGTCAAT TCAGGGTGGT GAATGTGAAA CCAGTAACGT TATACGATGT CGCAGAGTAT
      3001 GCCGGTGTCT CTTATCAGAC CGTTTCCCGC GTGGTGAACC AGGCCAGCCA CGTTTCTGCG
      3061 AAAACGCGGG AAAAAGTGGA AGCGGCGATG GCGGAGCTGA ATTACATTCC CAACCGCGTG
      3121 GCACAACAAC TGGCGGGCAA ACAGTCGTTG CTGATTGGCG TTGCCACCTC CAGTCTGGCC
      3181 CTGCACGCGC CGTCGCAAAT TGTCGCGGCG ATTAAATCTC GCGCCGATCA ACTGGGTGCC
      3241 AGCGTGGTGG TGTCGATGGT AGAACGAAGC GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC
      3301 AATCTTCTCG CGCAACGCGT CAGTGGGCTG ATCATTAACT ATCCGCTGGA TGACCAGGAT
      3361 GCCATTGCTG TGGAAGCTGC CTGCACTAAT GTTCCGGCGT TATTTCTTGA TGTCTCTGAC
      3421 CAGACACCCA TCAACAGTAT TATTTTCTCC CATGAAGACG GTACGCGACT GGGCGTGGAG
      3481 CATCTGGTCG CATTGGGTCA CCAGCAAATC GCGCTGTTAG CGGGCCCATT AAGTTCTGTC
      3541 TCGGCGCGTC TGCGTCTGGC TGGCTGGCAT AAATATCTCA CTCGCAATCA AATTCAGCCG
      3601 ATAGCGGAAC GGGAAGGCGA CTGGAGTGCC ATGTCCGGTT TTCAACAAAC CATGCAAATG
      3661 CTGAATGAGG GCATCGTTCC CACTGCGATG CTGGTTGCCA ACGATCAGAT GGCGCTGGGC
      3721 GCAATGCGCG CCATTACCGA GTCCGGGCTG CGCGTTGGTG CGGATATCTC GGTAGTGGGA
      3781 TACGACGATA CCGAAGACAG CTCATGTTAT ATCCCGCCGT TAACCACCAT CAAACAGGAT
      3841 TTTCGCCTGC TGGGGCAAAC CAGCGTGGAC CGCTTGCTGC AACTCTCTCA GGGCCAGGCG
      3901 GTGAAGGGCA ATCAGCTGTT GCCCGTCTCA CTGGTGAAAA GAAAAACCAC CCTGGCGCCC
      3961 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG
      4021 GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTAAGTT AGCTCACTCA
      4081 TTAGGCACCG GGATCTCGAC CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG
      4141 GTGGGCGCGG GGCATGACTA TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT
      4201 CGTAGGACAG GTGCCGGCAG CGCTCTGGGT CATTTTCGGC GAGGACCGCT TTCGCTGGAG
      4261 CGCGACGATG ATCGGCCTGT CGCTTGCGGT ATTCGGAATC TTGCACGCCC TCGCTCAAGC
      4321 CTTCGTCACT GGTCCCGCCA CCAAACGTTT CGGCGAGAAG CAGGCCATTA TCGCCGGCAT
      4381 GGCGGCCCCA CGGGTGCGCA TGATCGTGCT CCTGTCGTTG AGGACCCGGC TAGGCTGGCG
      4441 GGGTTGCCTT ACTGGTTAGC AGAATGAATC ACCGATACGC GAGCGAACGT GAAGCGACTG
      4501 CTGCTGCAAA ACGTCTGCGA CCTGAGCAAC AACATGAATG GTCTTCGGTT TCCGTGTTTC
      4561 GTAAAGTCTG GAAACGCGGA AGTCAGCGCC CTGCACCATT ATGTTCCGGA TCTGCATCGC
      4621 AGGATGCTGC TGGCTACCCT GTGGAACACC TACATCTGTA TTAACGAAGC GCTGGCATTG
      4681 ACCCTGAGTG ATTTTTCTCT GGTCCCGCCG CATCCATACC GCCAGTTGTT TACCCTCACA
      4741 ACGTTCCAGT AACCGGGCAT GTTCATCATC AGTAACCCGT ATCGTGAGCA TCCTCTCTCG
      4801 TTTCATCGGT ATCATTACCC CCATGAACAG AAATCCCCCT TACACGGAGG CATCAGTGAC
      4861 CAAACAGGAA AAAACCGCCC TTAACATGGC CCGCTTTATC AGAAGCCAGA CATTAACGCT
      4921 TCTGGAGAAA CTCAACGAGC TGGACGCGGA TGAACAGGCA GACATCTGTG AATCGCTTCA
      4981 CGACCACGCT GATGAGCTTT ACCGCAGCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA
      5041 CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG
      5101 CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC
      5161 CCAGTCACGT AGCGATAGCG GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT
      5221 GTACTGAGAG TGCACCATAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT
      5281 ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC
      5341 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG
      5401 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG
      5461 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC
      5521 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
      5581 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT
      5641 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG
      5701 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT
      5761 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC
      5821 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT
      5881 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC
      5941 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA
      6001 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
      6061 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC
      6121 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT
      6181 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC
      6241 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG
      6301 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG
      6361 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC
      6421 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA
      6481 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG
      6541 TTGCCATTGC TGCAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT
      6601 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA
      6661 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG
      6721 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA
      6781 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT
      6841 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA
      6901 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT
      6961 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT
      7021 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA
      7081 AATGTTGAAT ACTCATACTC TTCCTTTTTC AAATTATTGA AGCATTTATC AGGGTTATTG
      7141 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG
      7201 CACATTTCCC CGAAAAGTGC CACCTAAATT GTAAGCGTTA ATGTGAACCA TCACCCTAAT
      7261 CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC
      7321 GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA
      7381 AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC
      7441 CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCA
//