FEATURES             Location/Qualifiers
     CDS             2030..2989
                     /note="Lac I cds;Lac I cds"
     CDS             complement(5178..6038)
                     /note="bla cds;bla cds"
     rep_origin      4420..4420
                     /note="pBR322 origin of replication;pBR322 origin of replic
                     ation"
     promoter        complement(1500..1516)
                     /note="T7 Promoter;T7 Promoter"
     terminator      complement(26..72)
                     /note="T7 Terminator;T7 Terminator"
     misc_feature    complement(205..1428)
                     /note="cds"
     misc_feature    complement(250..267)
                     /note="His6"
     misc_feature    complement(1396..1425)
                     /note="His10"
     misc_feature    complement(274..1371)
                     /note="MBP"
     misc_feature    complement(223..243)
                     /note="TEV site"
     misc_feature    complement(205..234)
                     /note="LIC site"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTATCC ACTTCCAATA TTGGATTGGA AGTACAGGTT
       241 CTCGGTACAG TGGTGGTGGT GGTGGTGCGA ATTAGTCTGC GCGTCTTTCA GGGCTTCATC
       301 GACAGTCTGA CGACCGCTGG CGGCGTTGAT CACCGCAGTA CGCACGGCAT ACCAGAAAGC
       361 GGACATCTGC GGGATGTTCG GCATGATTTC ACCTTTCTGG GCGTTTTCCA TGGTGGCGGC
       421 AATACGTGGA TCTTTCGCCA ACTCTTCCTC GTAAGACTTC AGCGCTACGG CACCCAGCGG
       481 TTTGTCTTTA TTAACCGCTT CCAGACCTTC ATCAGTCAGC AGATAGTTTT CGAGGAACTC
       541 TTTTGCCAGC TCTTTGTTCG GACTGGCGGC GTTAATACCT GCGCTCAGCA CGCCAACGAA
       601 CGGTTTGGAT GGTTGACCCT TGAAGGTCGG CAGTACCGTT ACACCATAAT TCACTTTGCT
       661 GGTGTCGATG TTGGACCATG CCCACGGGCC GTTGATGGTC ATCGCTGTTT CGCCTTTATT
       721 AAAGGCAGCT TCTGCGATGG AGTAATCGGT GTCTGCATTC ATGTGTTTGT TTTTAATCAG
       781 GTCAACCAGG AAGGTCAGAC CCGCTTTCGC GCCAGCGTTA TCCACGCCCA CGTCTTTAAT
       841 GTCGTACTTG CCGTTTTCAT ACTTGAACGC ATAACCCCCG TCAGCAGCAA TCAGCGGCCA
       901 GGTGAAGTAC GGTTCTTGCA GGTTGAACAT CAGCGCGCTC TTACCTTTCG CTTTCAGTTC
       961 TTTATCCAGC GCCGGGATCT CTTCCCAGGT TTTTGGCGGG TTCGGCAGCA GATCTTTGTT
      1021 ATAAATCAGC GATAACGCTT CAACAGCGAT CGGGTAAGCA ATCAGCTTGC CGTTGTAACG
      1081 TACGGCATCC CAGGTAAACG GATACAGCTT GTCCTGGAAC GCTTTGTCCG GGGTGATTTC
      1141 AGCCAACAGG CCAGATTGAG CGTAGCCACC AAAGCGGTCG TGTGCCCAGA AGATAATGTC
      1201 AGGGCCATCG CCAGTTGCCG CAACCTGTGG GAATTTCTCT TCCAGTTTAT CCGGATGCTC
      1261 AACGGTGACT TTAATTCCGG TATCTTTCTC GAATTTCTTA CCGACTTCAG CGAGACCGTT
      1321 ATAGCCTTTA TCGCCGTTAA TCCAGATTAC CAGTTTACCT TCTTCGATTT TGGTACCCAG
      1381 ATCTACACCA GAAGAGTGGT GGTGGTGATG ATGATGATGA TGGTGCATAT GTATATCTCC
      1441 TTCTTAAAGT TAAACAAAAT TATTTCTAGA GGGGAATTGT TATCCGCTCA CAATTCCCCT
      1501 ATAGTGAGTC GTATTAATTT CGCGGGATCG AGATCGATCT CGATCCTCTA CGCCGGACGC
      1561 ATCGTGGCCG GCATCACCGG CGCCACAGGT GCGGTTGCTG GCGCCTATAT CGCCGACATC
      1621 ACCGATGGGG AAGATCGGGC TCGCCACTTC GGGCTCATGA GCGCTTGTTT CGGCGTGGGT
      1681 ATGGTGGCAG GCCCCGTGGC CGGGGGACTG TTGGGCGCCA TCTCCTTGCA TGCACCATTC
      1741 CTTGCGGCGG CGGTGCTCAA CGGCCTCAAC CTACTACTGG GCTGCTTCCT AATGCAGGAG
      1801 TCGCATAAGG GAGAGCGTCG AGATCCCGGA CACCATCGAA TGGCGCAAAA CCTTTCGCGG
      1861 TATGGCATGA TAGCGCCCGG AAGAGAGTCA ATTCAGGGTG GTGAATGTGA AACCAGTAAC
      1921 GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG ACCGTTTCCC GCGTGGTGAA
      1981 CCAGGCCAGC CACGTTTCTG CGAAAACGCG GGAAAAAGTG GAAGCGGCGA TGGCGGAGCT
      2041 GAATTACATT CCCAACCGCG TGGCACAACA ACTGGCGGGC AAACAGTCGT TGCTGATTGG
      2101 CGTTGCCACC TCCAGTCTGG CCCTGCACGC GCCGTCGCAA ATTGTCGCGG CGATTAAATC
      2161 TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG GTAGAACGAA GCGGCGTCGA
      2221 AGCCTGTAAA GCGGCGGTGC ACAATCTTCT CGCGCAACGC GTCAGTGGGC TGATCATTAA
      2281 CTATCCGCTG GATGACCAGG ATGCCATTGC TGTGGAAGCT GCCTGCACTA ATGTTCCGGC
      2341 GTTATTTCTT GATGTCTCTG ACCAGACACC CATCAACAGT ATTATTTTCT CCCATGAAGA
      2401 CGGTACGCGA CTGGGCGTGG AGCATCTGGT CGCATTGGGT CACCAGCAAA TCGCGCTGTT
      2461 AGCGGGCCCA TTAAGTTCTG TCTCGGCGCG TCTGCGTCTG GCTGGCTGGC ATAAATATCT
      2521 CACTCGCAAT CAAATTCAGC CGATAGCGGA ACGGGAAGGC GACTGGAGTG CCATGTCCGG
      2581 TTTTCAACAA ACCATGCAAA TGCTGAATGA GGGCATCGTT CCCACTGCGA TGCTGGTTGC
      2641 CAACGATCAG ATGGCGCTGG GCGCAATGCG CGCCATTACC GAGTCCGGGC TGCGCGTTGG
      2701 TGCGGATATC TCGGTAGTGG GATACGACGA TACCGAAGAC AGCTCATGTT ATATCCCGCC
      2761 GTTAACCACC ATCAAACAGG ATTTTCGCCT GCTGGGGCAA ACCAGCGTGG ACCGCTTGCT
      2821 GCAACTCTCT CAGGGCCAGG CGGTGAAGGG CAATCAGCTG TTGCCCGTCT CACTGGTGAA
      2881 AAGAAAAACC ACCCTGGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC
      2941 ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA
      3001 TTAATGTAAG TTAGCTCACT CATTAGGCAC CGGGATCTCG ACCGATGCCC TTGAGAGCCT
      3061 TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC TATCGTCGCC GCACTTATGA
      3121 CTGTCTTCTT TATCATGCAA CTCGTAGGAC AGGTGCCGGC AGCGCTCTGG GTCATTTTCG
      3181 GCGAGGACCG CTTTCGCTGG AGCGCGACGA TGATCGGCCT GTCGCTTGCG GTATTCGGAA
      3241 TCTTGCACGC CCTCGCTCAA GCCTTCGTCA CTGGTCCCGC CACCAAACGT TTCGGCGAGA
      3301 AGCAGGCCAT TATCGCCGGC ATGGCGGCCC CACGGGTGCG CATGATCGTG CTCCTGTCGT
      3361 TGAGGACCCG GCTAGGCTGG CGGGGTTGCC TTACTGGTTA GCAGAATGAA TCACCGATAC
      3421 GCGAGCGAAC GTGAAGCGAC TGCTGCTGCA AAACGTCTGC GACCTGAGCA ACAACATGAA
      3481 TGGTCTTCGG TTTCCGTGTT TCGTAAAGTC TGGAAACGCG GAAGTCAGCG CCCTGCACCA
      3541 TTATGTTCCG GATCTGCATC GCAGGATGCT GCTGGCTACC CTGTGGAACA CCTACATCTG
      3601 TATTAACGAA GCGCTGGCAT TGACCCTGAG TGATTTTTCT CTGGTCCCGC CGCATCCATA
      3661 CCGCCAGTTG TTTACCCTCA CAACGTTCCA GTAACCGGGC ATGTTCATCA TCAGTAACCC
      3721 GTATCGTGAG CATCCTCTCT CGTTTCATCG GTATCATTAC CCCCATGAAC AGAAATCCCC
      3781 CTTACACGGA GGCATCAGTG ACCAAACAGG AAAAAACCGC CCTTAACATG GCCCGCTTTA
      3841 TCAGAAGCCA GACATTAACG CTTCTGGAGA AACTCAACGA GCTGGACGCG GATGAACAGG
      3901 CAGACATCTG TGAATCGCTT CACGACCACG CTGATGAGCT TTACCGCAGC TGCCTCGCGC
      3961 GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT
      4021 GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG
      4081 GGTGTCGGGG CGCAGCCATG ACCCAGTCAC GTAGCGATAG CGGAGTGTAT ACTGGCTTAA
      4141 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATATGCGGTG TGAAATACCG
      4201 CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCTCTT CCGCTTCCTC GCTCACTGAC
      4261 TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA
      4321 CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA
      4381 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT
      4441 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA
      4501 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG
      4561 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA
      4621 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA
      4681 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG
      4741 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG
      4801 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG
      4861 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC
      4921 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG
      4981 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
      5041 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC
      5101 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
      5161 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
      5221 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
      5281 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA
      5341 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
      5401 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
      5461 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTGCAGGCA TCGTGGTGTC ACGCTCGTCG
      5521 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
      5581 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
      5641 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
      5701 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
      5761 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
      5821 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
      5881 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
      5941 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
      6001 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAAATTATT
      6061 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
      6121 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTAAA TTGTAAGCGT
      6181 TAATGTGAAC CATCACCCTA ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA AGCACTAAAT
      6241 CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG
      6301 AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC
      6361 ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTCCCAT
      6421 TCGCCA
//