FEATURES Location/Qualifiers misc_feature 204..235 /note="LIC Site;LIC Site" Cleavage-site 223..243 /note="TEV Site;TEV Site" Region 268..285 /note="His Tag;His Tag" CDS complement(205..930) /note="coding region;coding region" promoter complement(1002..1018) /note="T7 Promoter;T7 Promoter" terminator complement(26..72) /note="T7 Terminator;T7 Terminator" CDS 2400..3359 /note="Lac I cds;Lac I cds" CDS complement(5548..6408) /note="bla cds;bla cds" rep_origin 4760..4760 /note="pBR322 origin of replication;pBR322 origin of replic ation" Region complement(328..930) /note="osmY cds;osmY cds" Cleavage-site complement(286..306) /note="TVMV cleavage site;TVMV cleavage site" misc_feature complement(939..944) /note="ribosome binding site;ribosome binding site" CDS 1468..2094 /note="dsbA cds" promoter 1236..1467 /note="BL21 promoter" 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT 121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT 181 CGACGGAGCT CGAATTCGGA TCCGTTATCC ACTTCCAATA TTGGATTGGA AGTACAGGTT 241 CTCGGTACCC AGATCCACGC CAGAAGAGTG ATGATGATGG TGGTGAGACT GGAAACGCAC 301 GGTTTCCGAG CCTGCTTTTT TGTACAACTT AGTTTTCAGA TCATTTTTAA CGCTTTTCAC 361 ACCATCTACC GCTTTGGCGA TACTTTCAGC ACGGTCACTT TGTGCCTGAG AATCGACGGT 421 ACCGGAGAGC TGAACCACGC CGTCGGTGGT TTCAACTTTC ACATGACGGG AAGGGACGAT 481 ATCGTCCGCC AGCAGTTTGG CTTTGATTTC ACTGGTGGTG GCGGTGTCAC CCGCGTAGCC 541 CTTCACCGAG CCTTCTTTAG CGTCGCGAAC GTGCAGTTTG TCGCTGACAG AGGTCACCCC 601 TTCAACGCCT TTCGCCACTT TCACTGCCTC TTCGGCCTGG GCCTGGCTTT CAACGAAACC 661 GCTCAGGGTC ACGACTTTTT GATCGGTTTT TACAGAGATA TCGGTGCTCT TGATGTTGTC 721 ATGATCCACC AGGGCCGCCT TCACTTTCGC GGTGATGGCG CTGTCATCCA TGAAATTACC 781 GACTTTATTC ATAGAGCTAT CGACTTTTTG CCCTGCGCTT TCATTGGTAG TCTGCGCGTT 841 GTTTTCCGCG TAGGCAGAGC CGGTCGCGAC GGCAGAGGTC AACATTACAG CCAGCAGAGT 901 TTTCGAAATC TTCAGTCTTG TCATAGTCAT ATGTATATCT CCTTCTTAAA GTTAAACAAA 961 ATTATTTCTA GAGGGGAATT GTTATCCGCT CACAATTCCC CTATAGTGAG TCGTATTAAT 1021 TTCGCGGGAT CGAGATCGAT CTCGATCCTC TACGCCGGAC GCATCGTGGC CGGCATCACC 1081 GGCGCCACAG GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG GGAAGATCGG 1141 GCTCGCCACT TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC AGGCCCCGTG 1201 GCCGGGGGAC TGTTGGGCGC CATCTCCTTG CATGCTTCGA CACCGCTGAA ATCGGACTGA 1261 TTGAACCTTT ACGCGCCATG CGTTTGGTTT ATTATCTTGC CTGGCTAATG CGGCGTTGGG 1321 CTGATCCCGC GTTCCCGAAA AATTTCCCGT GGTTAACCGG GGAAGATTAC TGGCTACGAC 1381 AGACGGCGAC TTTTATAGAA CAGGCAAAAG TTCTACAAGA ACCCCCTTTG CAATTAACAC 1441 CTATGTATTA ATCGGAGAGA GTAGATCATG AAAAAGATTT GGCTGGCGCT GGCTGGTTTA 1501 GTTTTAGCGT TTAGCGCATC GGCGGCGCAG TATGAAGATG GTAAACAGTA CACTACCCTG 1561 GAAAAACCGG TAGCTGGCGC GCCGCAAGTG CTGGAGTTTT TCTCTTTCTT CTGCCCGCAC 1621 TGCTATCAGT TTGAAGAAGT TCTGCATATT TCTGATAATG TGAAGAAAAA ACTGCCGGAA 1681 GGCGTGAAGA TGACTAAATA CCACGTCAAC TTCATGGGTG GTGACCTGGG CAAAGATCTG 1741 ACTCAGGCAT GGGCTGTGGC GATGGCGCTG GGCGTGGAAG ACAAAGTCAC TGTTCCGCTG 1801 TTTGAAGGCG TACAGAAAAC CCAGACCATT CGTTCAGCAT CTGATATCCG CGATGTATTT 1861 ATCAACGCAG GTATTAAAGG TGAAGAGTAC GACGCGGCGT GGAACAGCTT CGTGGTCAAA 1921 TCTCTGGTCG CTCAGCAGGA AAAAGCTGCA GCTGACGTGC AATTGCGTGG CGTTCCGGCG 1981 ATGTTTGTTA ACGGTAAATA TCAGCTGAAT CCGCAGGGTA TGGATACCAG CAATATGGAT 2041 GTTTTTGTTC AGCAGTATGC TGATACAGTG AAATATCTGT CCGAGAAAAA ATAATAAGCA 2101 TGCACCATTC CTTGCGGCGG CGGTGCTCAA CGGCCTCAAC CTACTACTGG GCTGCTTCCT 2161 AATGCAGGAG TCGCATAAGG GAGAGCGTCG AGATCCCGGA CACCATCGAA TGGCGCAAAA 2221 CCTTTCGCGG TATGGCATGA TAGCGCCCGG AAGAGAGTCA ATTCAGGGTG GTGAATGTGA 2281 AACCAGTAAC GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG ACCGTTTCCC 2341 GCGTGGTGAA CCAGGCCAGC CACGTTTCTG CGAAAACGCG GGAAAAAGTG GAAGCGGCGA 2401 TGGCGGAGCT GAATTACATT CCCAACCGCG TGGCACAACA ACTGGCGGGC AAACAGTCGT 2461 TGCTGATTGG CGTTGCCACC TCCAGTCTGG CCCTGCACGC GCCGTCGCAA ATTGTCGCGG 2521 CGATTAAATC TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG GTAGAACGAA 2581 GCGGCGTCGA AGCCTGTAAA GCGGCGGTGC ACAATCTTCT CGCGCAACGC GTCAGTGGGC 2641 TGATCATTAA CTATCCGCTG GATGACCAGG ATGCCATTGC TGTGGAAGCT GCCTGCACTA 2701 ATGTTCCGGC GTTATTTCTT GATGTCTCTG ACCAGACACC CATCAACAGT ATTATTTTCT 2761 CCCATGAAGA CGGTACGCGA CTGGGCGTGG AGCATCTGGT CGCATTGGGT CACCAGCAAA 2821 TCGCGCTGTT AGCGGGCCCA TTAAGTTCTG TCTCGGCGCG TCTGCGTCTG GCTGGCTGGC 2881 ATAAATATCT CACTCGCAAT CAAATTCAGC CGATAGCGGA ACGGGAAGGC GACTGGAGTG 2941 CCATGTCCGG TTTTCAACAA ACCATGCAAA TGCTGAATGA GGGCATCGTT CCCACTGCGA 3001 TGCTGGTTGC CAACGATCAG ATGGCGCTGG GCGCAATGCG CGCCATTACC GAGTCCGGGC 3061 TGCGCGTTGG TGCGGATATC TCGGTAGTGG GATACGACGA TACCGAAGAC AGCTCATGTT 3121 ATATCCCGCC GTTAACCACC ATCAAACAGG ATTTTCGCCT GCTGGGGCAA ACCAGCGTGG 3181 ACCGCTTGCT GCAACTCTCT CAGGGCCAGG CGGTGAAGGG CAATCAGCTG TTGCCCGTCT 3241 CACTGGTGAA AAGAAAAACC ACCCTGGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT 3301 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG 3361 CGCAACGCAA TTAATGTAAG TTAGCTCACT CATTAGGCAC CGGGATCTCG ACCGATGCCC 3421 TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC TATCGTCGCC 3481 GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC AGGTGCCGGC AGCGCTCTGG 3541 GTCATTTTCG GCGAGGACCG CTTTCGCTGG AGCGCGACGA TGATCGGCCT GTCGCTTGCG 3601 GTATTCGGAA TCTTGCACGC CCTCGCTCAA GCCTTCGTCA CTGGTCCCGC CACCAAACGT 3661 TTCGGCGAGA AGCAGGCCAT TATCGCCGGC ATGGCGGCCC CACGGGTGCG CATGATCGTG 3721 CTCCTGTCGT TGAGGACCCG GCTAGGCTGG CGGGGTTGCC TTACTGGTTA GCAGAATGAA 3781 TCACCGATAC GCGAGCGAAC GTGAAGCGAC TGCTGCTGCA AAACGTCTGC GACCTGAGCA 3841 ACAACATGAA TGGTCTTCGG TTTCCGTGTT TCGTAAAGTC TGGAAACGCG GAAGTCAGCG 3901 CCCTGCACCA TTATGTTCCG GATCTGCATC GCAGGATGCT GCTGGCTACC CTGTGGAACA 3961 CCTACATCTG TATTAACGAA GCGCTGGCAT TGACCCTGAG TGATTTTTCT CTGGTCCCGC 4021 CGCATCCATA CCGCCAGTTG TTTACCCTCA CAACGTTCCA GTAACCGGGC ATGTTCATCA 4081 TCAGTAACCC GTATCGTGAG CATCCTCTCT CGTTTCATCG GTATCATTAC CCCCATGAAC 4141 AGAAATCCCC CTTACACGGA GGCATCAGTG ACCAAACAGG AAAAAACCGC CCTTAACATG 4201 GCCCGCTTTA TCAGAAGCCA GACATTAACG CTTCTGGAGA AACTCAACGA GCTGGACGCG 4261 GATGAACAGG CAGACATCTG TGAATCGCTT CACGACCACG CTGATGAGCT TTACCGCAGC 4321 TGCCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG 4381 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG 4441 GGTGTTGGCG GGTGTCGGGG CGCAGCCATG ACCCAGTCAC GTAGCGATAG CGGAGTGTAT 4501 ACTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATATGCGGTG 4561 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCTCTT CCGCTTCCTC 4621 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA 4681 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 4741 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 4801 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 4861 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 4921 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 4981 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 5041 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 5101 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 5161 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 5221 CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 5281 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 5341 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 5401 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 5461 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 5521 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC 5581 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC 5641 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC 5701 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG 5761 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG 5821 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTGCAGGCA TCGTGGTGTC 5881 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC 5941 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG 6001 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC 6061 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG 6121 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC 6181 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT 6241 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG 6301 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA 6361 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT 6421 TCAAATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 6481 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTAAA 6541 TTGTAAGCGT TAATGTGAAC CATCACCCTA ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA 6601 AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG GAAAGCCGGC 6661 GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG 6721 TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG 6781 CGCGTCCCAT TCGCCA //