FEATURES             Location/Qualifiers
     misc_feature    complement(245..265)
                     /note="LIC Site"
     Region          complement(208..225)
                     /note="His Tag"
     promoter        complement(327..343)
                     /note="T7 Promoter"
     Region          complement(140..157)
                     /note="His Tag;carboxy terminal His tag from pET-21a"
     terminator      complement(26..72)
                     /note="T7 Terminator"
     CDS             1884..2843
                     /note="Lac I cds"
     CDS             complement(5032..5892)
                     /note="bla cds"
     rep_origin      4274..4274
                     /note="pBR322 origin of replication"
     misc_feature    complement(232..252)
                     /note="TEV site"
     CDS             739..1449
                     /note="TVMV protease cds"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTAATG ATGATGATGG TGGTGCCCGG CGGATTGGAA
       241 GTACAGGTTC TCCCCGGGAG AGACTCCTTC TTAAAGTTAA ACAAAATTAT TTCTAGAGGG
       301 GAATTGTTAT CCGCTCACAA TTCCCCTATA GTGAGTCGTA TTAATTTCGC GGGATCGAGA
       361 TCGATCTCGA TCCTCTACGC CGGACGCATC GTGGCCGGCA TCACCGGCGC CACAGGTGCG
       421 GTTGCTGGCG CCTATATCGC CGACATCACC GATGGGGAAG ATCGGGCTCG CCACTTCGGG
       481 CTCATGAGCG CTTGTTTCGG CGTGGGTATG GTGGCAGGCC CCGTGGCCGG GGGACTGTTG
       541 GGCGCCATCT CCTTGCATGC GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA
       601 AAAATAGGCG TATCACGAGG CCCTTTCGTC TTCACCTCGA GTCCCTATCA GTGATAGAGA
       661 TTGACATCCC TATCAGTGAT AGAGATACTG AGCACATCAG CAGGACGCAC TGACCGAATT
       721 GATTTAAGGA GGTAACATAT GTCTAAAGCT TTGCTGAAGG GCGTGCGCGA TTTTAATCCG
       781 ATCTCTGCTT GCGTATGCCT GCTGGAAAAC TCCTCGGATG GTCATAGTGA ACGTCTGTTT
       841 GGCATTGGTT TTGGCCCGTA TATCATTGCC AACCAGCATC TGTTTCGTCG TAACAATGGC
       901 GAACTGACCA TCAAAACCAT GCATGGTGAA TTCAAAGTCA AAAACTCTAC CCAGCTGCAG
       961 ATGAAACCGG TTGAAGGCCG TGACATTATC GTTATCAAAA TGGCTAAAGA CTTCCCGCCG
      1021 TTCCCGCAGA AACTGAAATT CCGTCAGCCG ACCATCAAAG ATCGTGTGTG CATGGTGTCC
      1081 ACCAACTTTC AGCAGAAAAG CGTCTCGAGC CTGGTGTCTG AATCCTCTCA CATTGTGCAT
      1141 AAAGAAGACA CTTCTTTCTG GCAGCACTGG ATCACCACTA AAGATGGCCA GTGTGGCAGC
      1201 CCACTAGTTT CCATCATTGA TGGCAACATT CTGGGCATCC ACAGCCTGAC TCATACCACC
      1261 AACGGTAGCA ACTACTTCGT GGAATTTCCG GAAAAATTCG TGGCGACTTA TCTAGATGCC
      1321 GCGGATGGTT GGTGCAAAAA CTGGAAATTC AACGCGGATA AAATCAGCTG GGGTTCCTTT
      1381 ACCCTGGTTG AAGATGCGCC GGAAGATGAC TTCATGGCCA AAAAAACTGT TGCCGCCATC
      1441 ATGGACTAAT AAAGATCCCA TGGTACGCGT GCTAGAGGCA TCAAATAAAA CGAAAGGCTC
      1501 AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA
      1561 GGACAAATCC GCCGCCCTAG AGCATGCACC ATTCCTTGCG GCGGCGGTGC TCAACGGCCT
      1621 CAACCTACTA CTGGGCTGCT TCCTAATGCA GGAGTCGCAT AAGGGAGAGC GTCGAGATCC
      1681 CGGACACCAT CGAATGGCGC AAAACCTTTC GCGGTATGGC ATGATAGCGC CCGGAAGAGA
      1741 GTCAATTCAG GGTGGTGAAT GTGAAACCAG TAACGTTATA CGATGTCGCA GAGTATGCCG
      1801 GTGTCTCTTA TCAGACCGTT TCCCGCGTGG TGAACCAGGC CAGCCACGTT TCTGCGAAAA
      1861 CGCGGGAAAA AGTGGAAGCG GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC
      1921 AACAACTGGC GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC
      1981 ACGCGCCGTC GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG GGTGCCAGCG
      2041 TGGTGGTGTC GATGGTAGAA CGAAGCGGCG TCGAAGCCTG TAAAGCGGCG GTGCACAATC
      2101 TTCTCGCGCA ACGCGTCAGT GGGCTGATCA TTAACTATCC GCTGGATGAC CAGGATGCCA
      2161 TTGCTGTGGA AGCTGCCTGC ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA
      2221 CACCCATCAA CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC
      2281 TGGTCGCATT GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT TCTGTCTCGG
      2341 CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG CAATCAAATT CAGCCGATAG
      2401 CGGAACGGGA AGGCGACTGG AGTGCCATGT CCGGTTTTCA ACAAACCATG CAAATGCTGA
      2461 ATGAGGGCAT CGTTCCCACT GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA
      2521 TGCGCGCCAT TACCGAGTCC GGGCTGCGCG TTGGTGCGGA TATCTCGGTA GTGGGATACG
      2581 ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA CAGGATTTTC
      2641 GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT CTCTCAGGGC CAGGCGGTGA
      2701 AGGGCAATCA GCTGTTGCCC GTCTCACTGG TGAAAAGAAA AACCACCCTG GCGCCCAATA
      2761 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT
      2821 CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG
      2881 GCACCGGGAT CTCGACCGAT GCCCTTGAGA GCCTTCAACC CAGTCAGCTC CTTCCGGTGG
      2941 GCGCGGGGCA TGACTATCGT CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA
      3001 GGACAGGTGC CGGCAGCGCT CTGGGTCATT TTCGGCGAGG ACCGCTTTCG CTGGAGCGCG
      3061 ACGATGATCG GCCTGTCGCT TGCGGTATTC GGAATCTTGC ACGCCCTCGC TCAAGCCTTC
      3121 GTCACTGGTC CCGCCACCAA ACGTTTCGGC GAGAAGCAGG CCATTATCGC CGGCATGGCG
      3181 GCCCCACGGG TGCGCATGAT CGTGCTCCTG TCGTTGAGGA CCCGGCTAGG CTGGCGGGGT
      3241 TGCCTTACTG GTTAGCAGAA TGAATCACCG ATACGCGAGC GAACGTGAAG CGACTGCTGC
      3301 TGCAAAACGT CTGCGACCTG AGCAACAACA TGAATGGTCT TCGGTTTCCG TGTTTCGTAA
      3361 AGTCTGGAAA CGCGGAAGTC AGCGCCCTGC ACCATTATGT TCCGGATCTG CATCGCAGGA
      3421 TGCTGCTGGC TACCCTGTGG AACACCTACA TCTGTATTAA CGAAGCGCTG GCATTGACCC
      3481 TGAGTGATTT TTCTCTGGTC CCGCCGCATC CATACCGCCA GTTGTTTACC CTCACAACGT
      3541 TCCAGTAACC GGGCATGTTC ATCATCAGTA ACCCGTATCG TGAGCATCCT CTCTCGTTTC
      3601 ATCGGTATCA TTACCCCCAT GAACAGAAAT CCCCCTTACA CGGAGGCATC AGTGACCAAA
      3661 CAGGAAAAAA CCGCCCTTAA CATGGCCCGC TTTATCAGAA GCCAGACATT AACGCTTCTG
      3721 GAGAAACTCA ACGAGCTGGA CGCGGATGAA CAGGCAGACA TCTGTGAATC GCTTCACGAC
      3781 CACGCTGATG AGCTTTACCG CAGCTGCCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC
      3841 TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA
      3901 CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCGCAGC CATGACCCAG
      3961 TCACGTAGCG ATAGCGGAGT GTATACTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC
      4021 TGAGAGTGCA CCATATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
      4081 CATCAGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG
      4141 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA
      4201 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
      4261 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
      4321 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
      4381 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
      4441 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
      4501 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
      4561 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
      4621 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
      4681 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT
      4741 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
      4801 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
      4861 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      4921 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      4981 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      5041 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
      5101 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
      5161 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
      5221 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
      5281 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
      5341 CATTGCTGCA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
      5401 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
      5461 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
      5521 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
      5581 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
      5641 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
      5701 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
      5761 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
      5821 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
      5881 TTGAATACTC ATACTCTTCC TTTTTCAAAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
      5941 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
      6001 TTTCCCCGAA AAGTGCCACC TAAATTGTAA GCGTTAATGT GAACCATCAC CCTAATCAAG
      6061 TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT
      6121 TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG
      6181 AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC
      6241 CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC CCATTCGCCA 
//