COMMENT
     pMCSG65         5369 bp
     base vector:    pMCSG60
     pMCSG60 digested with Kpn I and Bgl II and ligated to a pair 
     of annealed oligos with compatible overhangs coding for the 
     Hemagglutinin epitope.
     T7 Prom::His6::HA::TEV::LIC1::LIC2::T7 Term
FEATURES             Location/Qualifiers
     misc_feature    complement(260..291)
                     /note="MCSG7 LIC site"
     Cleavage-site   complement(279..299)
                     /note="TEV Site"
     Region          complement(351..368)
                     /note="His Tag"
     promoter        complement(443..459)
                     /note="T7 Promoter"
     terminator      complement(26..72)
                     /note="T7 Terminator"
     CDS             973..1932
                     /note="Lac I cds"
     CDS             complement(4121..4981)
                     /note="bla cds"
     misc_feature    complement(223..244)
                     /note="MCSG26 LIC site"
     RBS             complement(243..248)
                     /note="ribosome binding site"
     RBS             complement(380..385)
                     /note="ribosome binding site"
     misc_feature    complement(307..332)
                     /note="HA tag"
     rep_origin      complement(3362..3950)
                     /note="pBR322 origin of replication"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTATCC ACTTCCAATG TTATGGTGGT GCCCGGGAGA
       241 GACTCCTTCT TAAAGTTAAG TTATCCACTT CCAATATTGG ATTGGAAGTA CAGGTTCTCG
       301 GTACCTGCGT AGTCCGGCAC ATCATACGGA TACAGATCTA CACCAGAAGA ATGATGATGA
       361 TGATGGTGCA TATGTATATC TCCTTCTTAA AGTTAAACAA AATTATTTCT AGAGGGGAAT
       421 TGTTATCCGC TCACAATTCC CCTATAGTGA GTCGTATTAA TTTCGCGGGA TCGAGATCGA
       481 TCTCGATCCT CTACGCCGGA CGCATCGTGG CCGGCATCAC CGGCGCCACA GGTGCGGTTG
       541 CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG GGCTCGCCAC TTCGGGCTCA
       601 TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT GGCCGGGGGA CTGTTGGGCG
       661 CCATCTCCTT GCATGCACCA TTCCTTGCGG CGGCGGTGCT CAACGGCCTC AACCTACTAC
       721 TGGGCTGCTT CCTAATGCAG GAGTCGCATA AGGGAGAGCG TCGAGATCCC GGACACCATC
       781 GAATGGCGCA AAACCTTTCG CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG
       841 GTGGTGAATG TGAAACCAGT AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT
       901 CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA
       961 GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG
      1021 GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG
      1081 CAAATTGTCG CGGCGATTAA ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG
      1141 ATGGTAGAAC GAAGCGGCGT CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA
      1201 CGCGTCAGTG GGCTGATCAT TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA
      1261 GCTGCCTGCA CTAATGTTCC GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC
      1321 AGTATTATTT TCTCCCATGA AGACGGTACG CGACTGGGCG TGGAGCATCT GGTCGCATTG
      1381 GGTCACCAGC AAATCGCGCT GTTAGCGGGC CCATTAAGTT CTGTCTCGGC GCGTCTGCGT
      1441 CTGGCTGGCT GGCATAAATA TCTCACTCGC AATCAAATTC AGCCGATAGC GGAACGGGAA
      1501 GGCGACTGGA GTGCCATGTC CGGTTTTCAA CAAACCATGC AAATGCTGAA TGAGGGCATC
      1561 GTTCCCACTG CGATGCTGGT TGCCAACGAT CAGATGGCGC TGGGCGCAAT GCGCGCCATT
      1621 ACCGAGTCCG GGCTGCGCGT TGGTGCGGAT ATCTCGGTAG TGGGATACGA CGATACCGAA
      1681 GACAGCTCAT GTTATATCCC GCCGTTAACC ACCATCAAAC AGGATTTTCG CCTGCTGGGG
      1741 CAAACCAGCG TGGACCGCTT GCTGCAACTC TCTCAGGGCC AGGCGGTGAA GGGCAATCAG
      1801 CTGTTGCCCG TCTCACTGGT GAAAAGAAAA ACCACCCTGG CGCCCAATAC GCAAACCGCC
      1861 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA
      1921 AGCGGGCAGT GAGCGCAACG CAATTAATGT AAGTTAGCTC ACTCATTAGG CACCGGGATC
      1981 TCGACCGATG CCCTTGAGAG CCTTCAACCC AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT
      2041 GACTATCGTC GCCGCACTTA TGACTGTCTT CTTTATCATG CAACTCGTAG GACAGGTGCC
      2101 GGCAGCGCTC TGGGTCATTT TCGGCGAGGA CCGCTTTCGC TGGAGCGCGA CGATGATCGG
      2161 CCTGTCGCTT GCGGTATTCG GAATCTTGCA CGCCCTCGCT CAAGCCTTCG TCACTGGTCC
      2221 CGCCACCAAA CGTTTCGGCG AGAAGCAGGC CATTATCGCC GGCATGGCGG CCCCACGGGT
      2281 GCGCATGATC GTGCTCCTGT CGTTGAGGAC CCGGCTAGGC TGGCGGGGTT GCCTTACTGG
      2341 TTAGCAGAAT GAATCACCGA TACGCGAGCG AACGTGAAGC GACTGCTGCT GCAAAACGTC
      2401 TGCGACCTGA GCAACAACAT GAATGGTCTT CGGTTTCCGT GTTTCGTAAA GTCTGGAAAC
      2461 GCGGAAGTCA GCGCCCTGCA CCATTATGTT CCGGATCTGC ATCGCAGGAT GCTGCTGGCT
      2521 ACCCTGTGGA ACACCTACAT CTGTATTAAC GAAGCGCTGG CATTGACCCT GAGTGATTTT
      2581 TCTCTGGTCC CGCCGCATCC ATACCGCCAG TTGTTTACCC TCACAACGTT CCAGTAACCG
      2641 GGCATGTTCA TCATCAGTAA CCCGTATCGT GAGCATCCTC TCTCGTTTCA TCGGTATCAT
      2701 TACCCCCATG AACAGAAATC CCCCTTACAC GGAGGCATCA GTGACCAAAC AGGAAAAAAC
      2761 CGCCCTTAAC ATGGCCCGCT TTATCAGAAG CCAGACATTA ACGCTTCTGG AGAAACTCAA
      2821 CGAGCTGGAC GCGGATGAAC AGGCAGACAT CTGTGAATCG CTTCACGACC ACGCTGATGA
      2881 GCTTTACCGC AGCTGCCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA
      2941 GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA
      3001 GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA
      3061 TAGCGGAGTG TATACTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC
      3121 CATATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT
      3181 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT
      3241 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA
      3301 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT
      3361 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT
      3421 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC
      3481 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA
      3541 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT
      3601 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA
      3661 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG
      3721 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC
      3781 CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA
      3841 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG
      3901 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT
      3961 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG
      4021 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA
      4081 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG
      4141 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG
      4201 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC
      4261 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG
      4321 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG
      4381 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTGCAG
      4441 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT
      4501 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC
      4561 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC
      4621 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA
      4681 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC
      4741 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT
      4801 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC
      4861 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA
      4921 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA
      4981 TACTCTTCCT TTTTCAAATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
      5041 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
      5101 AGTGCCACCT AAATTGTAAG CGTTAATGTG AACCATCACC CTAATCAAGT TTTTTGGGGT
      5161 CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC
      5221 GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA
      5281 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG
      5341 CGCCGCTACA GGGCGCGTCC CATTCGCCA
//