COMMENT
     pMCSG69         5963 bp
     base vector:    pMCSG53
     LIC region plus a portion of the beta-lactamase gene from pMCSG19 removed
     by digestion with BglI and XbaI then ligated into pMCSG53 digested with the
     same enzymes. Product is pMCSG19 with truncated ori and the argU & ileX 
     tRNA genes
FEATURES             Location/Qualifiers
     tRNA            complement(2114..2265)
                     /note="ileX gene"
     rep_origin      complement(3956..4544)
                     /note="pBR322 origin of replication"
     promoter        complement(1515..1531)
                     /note="T7 Promoter"
     CDS             2568..3527
                     /note="Lac I cds"
     CDS             complement(4715..5575)
                     /note="bla cds"
     tRNA            complement(1598..1954)
                     /note="argU tRNA"
     misc_feature    complement(204..235)
                     /note="LIC Site"
     Cleavage-site   complement(223..243)
                     /note="TEV Site"
     Region          complement(268..285)
                     /note="His Tag"
     terminator      complement(26..72)
                     /note="T7 Terminator"
     Region          complement(337..1443)
                     /note="maltose binding protein"
     Cleavage-site   complement(286..306)
                     /note="TVMV cleavage site"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTATCC ACTTCCAATA TTGGATTGGA AGTACAGGTT
       241 CTCGGTACCC AGATCCACGC CAGAAGAGTG ATGATGATGG TGGTGAGACT GGAAACGCAC
       301 GGTTTCCGAG CCTGCTTTTT TGTACAAACT TGTGATCGAA TTAGTCTGCG CGTCTTTCAG
       361 GGCTTCATCG ACAGTCTGAC GACCGCTGGC GGCGTTGATC ACCGCAGTAC GCACGGCATA
       421 CCAGAAAGCG GACATCTGCG GGATGTTCGG CATGATTTCA CCTTTCTGGG CGTTTTCCAT
       481 GGTGGCGGCA ATACGTGGAT CTTTCGCCAA CTCTTCCTCG TAAGACTTCA GCGCTACGGC
       541 ACCCAGCGGT TTGTCTTTAT TAACCGCTTC CAGACCTTCA TCAGTCAGCA GATAGTTTTC
       601 GAGGAACTCT TTTGCCAGCT CTTTGTTCGG ACTGGCGGCG TTAATACCTG CGCTCAGCAC
       661 GCCAACGAAC GGTTTGGATG GTTGACCCTT GAAGGTCGGC AGTACCGTTA CACCATAATT
       721 CACTTTGCTG GTGTCGATGT TGGACCATGC CCACGGGCCG TTGATGGTCA TCGCTGTTTC
       781 GCCTTTATTA AAGGCAGCTT CTGCGATGGA GTAATCGGTG TCTGCATTCA TGTGTTTGTT
       841 TTTAATCAGG TCAACCAGGA AGGTCAGACC CGCTTTCGCG CCAGCGTTAT CCACGCCCAC
       901 GTCTTTAATG TCGTACTTGC CGTTTTCATA CTTGAACGCA TAACCCCCGT CAGCAGCAAT
       961 CAGCGGCCAG GTGAAGTACG GTTCTTGCAG GTTGAACATC AGCGCGCTCT TACCTTTCGC
      1021 TTTCAGTTCT TTATCCAGCG CCGGGATCTC TTCCCAGGTT TTTGGCGGGT TCGGCAGCAG
      1081 ATCTTTGTTA TAAATCAGCG ATAACGCTTC AACAGCGATC GGGTAAGCAA TCAGCTTGCC
      1141 GTTGTAACGT ACGGCATCCC AGGTAAACGG ATACAGCTTG TCCTGGAACG CTTTGTCCGG
      1201 GGTGATTTCA GCCAACAGGC CAGATTGAGC GTAGCCACCA AAGCGGTCGT GTGCCCAGAA
      1261 GATAATGTCA GGGCCATCGC CAGTTGCCGC AACCTGTGGG AATTTCTCTT CCAGTTTATC
      1321 CGGATGCTCA ACGGTGACTT TAATTCCGGT ATCTTTCTCG AATTTCTTAC CGACTTCAGC
      1381 GAGACCGTTA TAGCCTTTAT CGCCGTTAAT CCAGATTACC AGTTTACCTT CTTCGATTTT
      1441 CATATGTATA TCTCCTTCTT AAAGTTAAAC AAAATTATTT CTAGAGGGGA ATTGTTATCC
      1501 GCTCACAATT CCCCTATAGT GAGTCGTATT AATTTCGCGG GATCGAGATC GATCTCGATC
      1561 CTCTACGCCG GACGCATCGT GGCCGGCATC ACCGGTGGAT TCAGTCAGGC GTCCCATTAT
      1621 CAGTGCTTCA GGAAATGGGC GGATGGGAGT CCATAGAAAT GGTTCGTAGG TATGCTCACC
      1681 TTGCGCCTAA TCATTTGACA GAGCATGCGA GGAAAATAGA CGACATTTTT GGTGATAATG
      1741 TCCCAAATAT GTCCCACTCT GGAATTATGG AGGATATAAA GAAGGCGTAA CTGATTGAAT
      1801 TGTAATGGCG CGCCCTGCAG GATTCGAACC TGCGGCCCAC GACTTAGAAG GTCGTTGCTC
      1861 TATCCAACTG AGCTAAGGGC GCGTTGATAC CGCAATGCGG TGTAATCGCG TGAATTATAC
      1921 GGTCAACCCT TGCTGAGTCA ATGGCTTTTG ATCTCGCCGG CGCCACAGGT GCGGTTGCTG
      1981 GCGCCTATAT CGCCGACATC ACCGATGGGG AAGATCGGGC TCGCCACTTC GGGCTCATGA
      2041 GCGCTTGTTT CGGCGTGGGT ATGGTGGCAG GCCCCGTGGC CGGGGGACTG TTGGGCGCCA
      2101 TCTCCTTGCA TGCAAAAAGG CTGACGATTT CTCGTCAGCC TTTGCTATAT CTGGTGGCCC
      2161 CTGCTGGACT TGAACCAGCG ACCAAGCGAT TATGAGTCGC CTGCTCTAAC CACTGAGCTA
      2221 AGGGGCCATG GTAGCGGATT ATAAAGTAAC TCCGTGTCGC AATCCGCATG CACCATTCCT
      2281 TGCGGCGGCG GTGCTCAACG GCCTCAACCT ACTACTGGGC TGCTTCCTAA TGCAGGAGTC
      2341 GCATAAGGGA GAGCGTCGAG ATCCCGGACA CCATCGAATG GCGCAAAACC TTTCGCGGTA
      2401 TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT GAATGTGAAA CCAGTAACGT
      2461 TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC CGTTTCCCGC GTGGTGAACC
      2521 AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA AGCGGCGATG GCGGAGCTGA
      2581 ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA ACAGTCGTTG CTGATTGGCG
      2641 TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT TGTCGCGGCG ATTAAATCTC
      2701 GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT AGAACGAAGC GGCGTCGAAG
      2761 CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT CAGTGGGCTG ATCATTAACT
      2821 ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC CTGCACTAAT GTTCCGGCGT
      2881 TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT TATTTTCTCC CATGAAGACG
      2941 GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA CCAGCAAATC GCGCTGTTAG
      3001 CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC TGGCTGGCAT AAATATCTCA
      3061 CTCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA CTGGAGTGCC ATGTCCGGTT
      3121 TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC CACTGCGATG CTGGTTGCCA
      3181 ACGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACCGA GTCCGGGCTG CGCGTTGGTG
      3241 CGGATATCTC GGTAGTGGGA TACGACGATA CCGAAGACAG CTCATGTTAT ATCCCGCCGT
      3301 TAACCACCAT CAAACAGGAT TTTCGCCTGC TGGGGCAAAC CAGCGTGGAC CGCTTGCTGC
      3361 AACTCTCTCA GGGCCAGGCG GTGAAGGGCA ATCAGCTGTT GCCCGTCTCA CTGGTGAAAA
      3421 GAAAAACCAC CCTGGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT
      3481 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT
      3541 AATGTAAGTT AGCTCACTCA TTAGGCACCG GGATCTCGAC CGATGCCCTT GAGAGCCTTC
      3601 AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA CAGTCACGTA GCGATAGCGG
      3661 AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATA
      3721 TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCTCTTCCG
      3781 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
      3841 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
      3901 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
      3961 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
      4021 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
      4081 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
      4141 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
      4201 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
      4261 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
      4321 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
      4381 ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
      4441 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
      4501 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
      4561 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
      4621 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
      4681 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC
      4741 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
      4801 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC
      4861 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
      4921 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA
      4981 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT GCAGGCATCG
      5041 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
      5101 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG
      5161 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
      5221 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT
      5281 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA
      5341 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
      5401 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC
      5461 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
      5521 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT
      5581 TCCTTTTTCA AATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
      5641 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
      5701 ACCTAAATTG TAAGCGTTAA TGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT
      5761 GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA
      5821 AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC
      5881 TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC
      5941 TACAGGGCGC GTCCCATTCG CCA
//