COMMENT
     pMCSG70         5911 bp
     base vector:    pMCSG53
     LIC region plus a portion of the beta-lactamase gene from pMCSG29 removed
     by digestion with BglI and XbaI then ligated into pMCSG53 digested with the
     same enzymes. Product is pMCSG29 with truncated ori and the argU & ileX 
     tRNA genes
FEATURES             Location/Qualifiers
     tRNA            complement(2062..2213)
                     /note="ileX gene"
     rep_origin      complement(3904..4492)
                     /note="pBR322 origin of replication"
     CDS             2516..3475
                     /note="Lac I cds"
     CDS             complement(4663..5523)
                     /note="bla cds"
     tRNA            complement(1546..1902)
                     /note="argU tRNA"
     misc_feature    complement(1382..1400)
                     /note="LIC Site"
     Region          complement(1332..1361)
                     /note="His10 Tag"
     promoter        complement(1463..1479)
                     /note="T7 Promoter"
     terminator      complement(26..72)
                     /note="T7 Terminator"
     Cleavage-site   complement(1311..1331)
                     /note="TVMV site"
     misc_feature    complement(204..1310)
                     /note="MBP"
     Cleavage-site   complement(1368..1388)
                     /note="TEV Site"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCTTACGAA TTAGTCTGCG CGTCTTTCAG GGCTTCATCG
       241 ACAGTCTGAC GACCGCTGGC GGCGTTGATC ACCGCAGTAC GCACGGCATA CCAGAAAGCG
       301 GACATCTGCG GGATGTTCGG CATGATTTCA CCTTTCTGGG CGTTTTCCAT GGTGGCGGCA
       361 ATACGTGGAT CTTTCGCCAA CTCTTCCTCG TAAGACTTCA GCGCTACGGC ACCCAGCGGT
       421 TTGTCTTTAT TAACCGCTTC CAGACCTTCA TCAGTCAGCA GATAGTTTTC GAGGAACTCT
       481 TTTGCCAGCT CTTTGTTCGG ACTGGCGGCG TTAATACCTG CGCTCAGCAC GCCAACGAAC
       541 GGTTTGGATG GTTGACCCTT GAAGGTCGGC AGTACCGTTA CACCATAATT CACTTTGCTG
       601 GTGTCGATGT TGGACCATGC CCACGGGCCG TTGATGGTCA TCGCTGTTTC GCCTTTATTA
       661 AAGGCAGCTT CTGCGATGGA GTAATCGGTG TCTGCATTCA TGTGTTTGTT TTTAATCAGG
       721 TCAACCAGGA AGGTCAGACC CGCTTTCGCG CCAGCGTTAT CCACGCCCAC GTCTTTAATG
       781 TCGTACTTGC CGTTTTCATA CTTGAACGCA TAACCCCCGT CAGCAGCAAT CAGCGGCCAG
       841 GTGAAGTACG GTTCTTGCAG GTTGAACATC AGCGCGCTCT TACCTTTCGC TTTCAGTTCT
       901 TTATCCAGCG CCGGGATCTC TTCCCAGGTT TTTGGCGGGT TCGGCAGCAG ATCTTTGTTA
       961 TAAATCAGCG ATAACGCTTC AACAGCGATC GGGTAAGCAA TCAGCTTGCC GTTGTAACGT
      1021 ACGGCATCCC AGGTAAACGG ATACAGCTTG TCCTGGAACG CTTTGTCCGG GGTGATTTCA
      1081 GCCAACAGGC CAGATTGAGC GTAGCCACCA AAGCGGTCGT GTGCCCAGAA GATAATGTCA
      1141 GGGCCATCGC CAGTTGCCGC AACCTGTGGG AATTTCTCTT CCAGTTTATC CGGATGCTCA
      1201 ACGGTGACTT TAATTCCGGT ATCTTTCTCG AATTTCTTAC CGACTTCAGC GAGACCGTTA
      1261 TAGCCTTTAT CGCCGTTAAT CCAGATTACC AGTTTACCTT CTTCGATTTT AGACTGGAAA
      1321 CGCACGGTTT CGTGATGGTG GTGATGATGA TGATGGTGGT GCCCGGCGGA TTGGAAGTAC
      1381 AGGTTCTCCC CGGGAGAGAC TCCTTCTTAA AGTTAAACAA AATTATTTCT AGAGGGGAAT
      1441 TGTTATCCGC TCACAATTCC CCTATAGTGA GTCGTATTAA TTTCGCGGGA TCGAGATCGA
      1501 TCTCGATCCT CTACGCCGGA CGCATCGTGG CCGGCATCAC CGGTGGATTC AGTCAGGCGT
      1561 CCCATTATCA GTGCTTCAGG AAATGGGCGG ATGGGAGTCC ATAGAAATGG TTCGTAGGTA
      1621 TGCTCACCTT GCGCCTAATC ATTTGACAGA GCATGCGAGG AAAATAGACG ACATTTTTGG
      1681 TGATAATGTC CCAAATATGT CCCACTCTGG AATTATGGAG GATATAAAGA AGGCGTAACT
      1741 GATTGAATTG TAATGGCGCG CCCTGCAGGA TTCGAACCTG CGGCCCACGA CTTAGAAGGT
      1801 CGTTGCTCTA TCCAACTGAG CTAAGGGCGC GTTGATACCG CAATGCGGTG TAATCGCGTG
      1861 AATTATACGG TCAACCCTTG CTGAGTCAAT GGCTTTTGAT CTCGCCGGCG CCACAGGTGC
      1921 GGTTGCTGGC GCCTATATCG CCGACATCAC CGATGGGGAA GATCGGGCTC GCCACTTCGG
      1981 GCTCATGAGC GCTTGTTTCG GCGTGGGTAT GGTGGCAGGC CCCGTGGCCG GGGGACTGTT
      2041 GGGCGCCATC TCCTTGCATG CAAAAAGGCT GACGATTTCT CGTCAGCCTT TGCTATATCT
      2101 GGTGGCCCCT GCTGGACTTG AACCAGCGAC CAAGCGATTA TGAGTCGCCT GCTCTAACCA
      2161 CTGAGCTAAG GGGCCATGGT AGCGGATTAT AAAGTAACTC CGTGTCGCAA TCCGCATGCA
      2221 CCATTCCTTG CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG
      2281 CAGGAGTCGC ATAAGGGAGA GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT
      2341 TCGCGGTATG GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC
      2401 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT
      2461 GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC
      2521 GGAGCTGAAT TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT
      2581 GATTGGCGTT GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT
      2641 TAAATCTCGC GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG
      2701 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT
      2761 CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT
      2821 TCCGGCGTTA TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA
      2881 TGAAGACGGT ACGCGACTGG GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC
      2941 GCTGTTAGCG GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA
      3001 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT
      3061 GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT
      3121 GGTTGCCAAC GATCAGATGG CGCTGGGCGC AATGCGCGCC ATTACCGAGT CCGGGCTGCG
      3181 CGTTGGTGCG GATATCTCGG TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT
      3241 CCCGCCGTTA ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG
      3301 CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC CCGTCTCACT
      3361 GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC
      3421 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA
      3481 ACGCAATTAA TGTAAGTTAG CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA
      3541 GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTACA GTCACGTAGC
      3601 GATAGCGGAG TGTATACTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      3661 ACCATATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG
      3721 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT
      3781 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
      3841 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC
      3901 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
      3961 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
      4021 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
      4081 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
      4141 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
      4201 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
      4261 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
      4321 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
      4381 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
      4441 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
      4501 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
      4561 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
      4621 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG
      4681 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT
      4741 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
      4801 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC
      4861 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
      4921 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTGC
      4981 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
      5041 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
      5101 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
      5161 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
      5221 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT
      5281 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
      5341 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
      5401 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
      5461 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
      5521 CATACTCTTC CTTTTTCAAA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
      5581 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
      5641 AAAGTGCCAC CTAAATTGTA AGCGTTAATG TGAACCATCA CCCTAATCAA GTTTTTTGGG
      5701 GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG
      5761 ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC
      5821 TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA
      5881 TGCGCCGCTA CAGGGCGCGT CCCATTCGCC A
//