COMMENT
     pMCSG71         5940 bp
     base vector:    pMCSG53
     LIC region plus a portion of the beta-lactamase gene from pMCSG32 removed
     by digestion with BglI and XbaI then ligated into pMCSG53 digested with the
     same enzymes. Product is pMCSG32 with truncated ori and the argU & ileX 
     tRNA genes
FEATURES             Location/Qualifiers
     tRNA            complement(2091..2242)
                     /note="ileX gene"
     rep_origin      complement(3933..4521)
                     /note="pBR322 origin of replication"
     CDS             2545..3504
                     /note="Lac I cds"
     CDS             complement(4692..5552)
                     /note="bla cds"
     tRNA            complement(1575..1931)
                     /note="argU tRNA"
     misc_feature    complement(245..265)
                     /note="LIC Site"
     Region          complement(208..225)
                     /note="His Tag"
     promoter        complement(1492..1508)
                     /note="T7 Promoter"
     terminator      complement(26..72)
                     /note="T7 Terminator"
     misc_feature    complement(232..252)
                     /note="TEV site"
     misc_feature    complement(263..283)
                     /note="TVMV site"
     misc_feature    complement(314..1420)
                     /note="MBP cds"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTAATG ATGATGATGG TGGTGCCCGG CGGATTGGAA
       241 GTACAGGTTC TCCCCGGGAG AGACTCTGGA AACGCACGGT TTCCGAGCCT GCTTTTTTGT
       301 ACAAACTTGT GATCGAATTA GTCTGCGCGT CTTTCAGGGC TTCATCGACA GTCTGACGAC
       361 CGCTGGCGGC GTTGATCACC GCAGTACGCA CGGCATACCA GAAAGCGGAC ATCTGCGGGA
       421 TGTTCGGCAT GATTTCACCT TTCTGGGCGT TTTCCATGGT GGCGGCAATA CGTGGATCTT
       481 TCGCCAACTC TTCCTCGTAA GACTTCAGCG CTACGGCACC CAGCGGTTTG TCTTTATTAA
       541 CCGCTTCCAG ACCTTCATCA GTCAGCAGAT AGTTTTCGAG GAACTCTTTT GCCAGCTCTT
       601 TGTTCGGACT GGCGGCGTTA ATACCTGCGC TCAGCACGCC AACGAACGGT TTGGATGGTT
       661 GACCCTTGAA GGTCGGCAGT ACCGTTACAC CATAATTCAC TTTGCTGGTG TCGATGTTGG
       721 ACCATGCCCA CGGGCCGTTG ATGGTCATCG CTGTTTCGCC TTTATTAAAG GCAGCTTCTG
       781 CGATGGAGTA ATCGGTGTCT GCATTCATGT GTTTGTTTTT AATCAGGTCA ACCAGGAAGG
       841 TCAGACCCGC TTTCGCGCCA GCGTTATCCA CGCCCACGTC TTTAATGTCG TACTTGCCGT
       901 TTTCATACTT GAACGCATAA CCCCCGTCAG CAGCAATCAG CGGCCAGGTG AAGTACGGTT
       961 CTTGCAGGTT GAACATCAGC GCGCTCTTAC CTTTCGCTTT CAGTTCTTTA TCCAGCGCCG
      1021 GGATCTCTTC CCAGGTTTTT GGCGGGTTCG GCAGCAGATC TTTGTTATAA ATCAGCGATA
      1081 ACGCTTCAAC AGCGATCGGG TAAGCAATCA GCTTGCCGTT GTAACGTACG GCATCCCAGG
      1141 TAAACGGATA CAGCTTGTCC TGGAACGCTT TGTCCGGGGT GATTTCAGCC AACAGGCCAG
      1201 ATTGAGCGTA GCCACCAAAG CGGTCGTGTG CCCAGAAGAT AATGTCAGGG CCATCGCCAG
      1261 TTGCCGCAAC CTGTGGGAAT TTCTCTTCCA GTTTATCCGG ATGCTCAACG GTGACTTTAA
      1321 TTCCGGTATC TTTCTCGAAT TTCTTACCGA CTTCAGCGAG ACCGTTATAG CCTTTATCGC
      1381 CGTTAATCCA GATTACCAGT TTACCTTCTT CGATTTTCAT ATGTATATCT CCTTCTTAAA
      1441 GTTAAACAAA ATTATTTCTA GAGGGGAATT GTTATCCGCT CACAATTCCC CTATAGTGAG
      1501 TCGTATTAAT TTCGCGGGAT CGAGATCGAT CTCGATCCTC TACGCCGGAC GCATCGTGGC
      1561 CGGCATCACC GGTGGATTCA GTCAGGCGTC CCATTATCAG TGCTTCAGGA AATGGGCGGA
      1621 TGGGAGTCCA TAGAAATGGT TCGTAGGTAT GCTCACCTTG CGCCTAATCA TTTGACAGAG
      1681 CATGCGAGGA AAATAGACGA CATTTTTGGT GATAATGTCC CAAATATGTC CCACTCTGGA
      1741 ATTATGGAGG ATATAAAGAA GGCGTAACTG ATTGAATTGT AATGGCGCGC CCTGCAGGAT
      1801 TCGAACCTGC GGCCCACGAC TTAGAAGGTC GTTGCTCTAT CCAACTGAGC TAAGGGCGCG
      1861 TTGATACCGC AATGCGGTGT AATCGCGTGA ATTATACGGT CAACCCTTGC TGAGTCAATG
      1921 GCTTTTGATC TCGCCGGCGC CACAGGTGCG GTTGCTGGCG CCTATATCGC CGACATCACC
      1981 GATGGGGAAG ATCGGGCTCG CCACTTCGGG CTCATGAGCG CTTGTTTCGG CGTGGGTATG
      2041 GTGGCAGGCC CCGTGGCCGG GGGACTGTTG GGCGCCATCT CCTTGCATGC AAAAAGGCTG
      2101 ACGATTTCTC GTCAGCCTTT GCTATATCTG GTGGCCCCTG CTGGACTTGA ACCAGCGACC
      2161 AAGCGATTAT GAGTCGCCTG CTCTAACCAC TGAGCTAAGG GGCCATGGTA GCGGATTATA
      2221 AAGTAACTCC GTGTCGCAAT CCGCATGCAC CATTCCTTGC GGCGGCGGTG CTCAACGGCC
      2281 TCAACCTACT ACTGGGCTGC TTCCTAATGC AGGAGTCGCA TAAGGGAGAG CGTCGAGATC
      2341 CCGGACACCA TCGAATGGCG CAAAACCTTT CGCGGTATGG CATGATAGCG CCCGGAAGAG
      2401 AGTCAATTCA GGGTGGTGAA TGTGAAACCA GTAACGTTAT ACGATGTCGC AGAGTATGCC
      2461 GGTGTCTCTT ATCAGACCGT TTCCCGCGTG GTGAACCAGG CCAGCCACGT TTCTGCGAAA
      2521 ACGCGGGAAA AAGTGGAAGC GGCGATGGCG GAGCTGAATT ACATTCCCAA CCGCGTGGCA
      2581 CAACAACTGG CGGGCAAACA GTCGTTGCTG ATTGGCGTTG CCACCTCCAG TCTGGCCCTG
      2641 CACGCGCCGT CGCAAATTGT CGCGGCGATT AAATCTCGCG CCGATCAACT GGGTGCCAGC
      2701 GTGGTGGTGT CGATGGTAGA ACGAAGCGGC GTCGAAGCCT GTAAAGCGGC GGTGCACAAT
      2761 CTTCTCGCGC AACGCGTCAG TGGGCTGATC ATTAACTATC CGCTGGATGA CCAGGATGCC
      2821 ATTGCTGTGG AAGCTGCCTG CACTAATGTT CCGGCGTTAT TTCTTGATGT CTCTGACCAG
      2881 ACACCCATCA ACAGTATTAT TTTCTCCCAT GAAGACGGTA CGCGACTGGG CGTGGAGCAT
      2941 CTGGTCGCAT TGGGTCACCA GCAAATCGCG CTGTTAGCGG GCCCATTAAG TTCTGTCTCG
      3001 GCGCGTCTGC GTCTGGCTGG CTGGCATAAA TATCTCACTC GCAATCAAAT TCAGCCGATA
      3061 GCGGAACGGG AAGGCGACTG GAGTGCCATG TCCGGTTTTC AACAAACCAT GCAAATGCTG
      3121 AATGAGGGCA TCGTTCCCAC TGCGATGCTG GTTGCCAACG ATCAGATGGC GCTGGGCGCA
      3181 ATGCGCGCCA TTACCGAGTC CGGGCTGCGC GTTGGTGCGG ATATCTCGGT AGTGGGATAC
      3241 GACGATACCG AAGACAGCTC ATGTTATATC CCGCCGTTAA CCACCATCAA ACAGGATTTT
      3301 CGCCTGCTGG GGCAAACCAG CGTGGACCGC TTGCTGCAAC TCTCTCAGGG CCAGGCGGTG
      3361 AAGGGCAATC AGCTGTTGCC CGTCTCACTG GTGAAAAGAA AAACCACCCT GGCGCCCAAT
      3421 ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT
      3481 TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTAAGTTAGC TCACTCATTA
      3541 GGCACCGGGA TCTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG
      3601 GGCGCGGGGC ATGACTACAG TCACGTAGCG ATAGCGGAGT GTATACTGGC TTAACTATGC
      3661 GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATATGC GGTGTGAAAT ACCGCACAGA
      3721 TGCGTAAGGA GAAAATACCG CATCAGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG
      3781 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA
      3841 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC
      3901 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG
      3961 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC
      4021 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC
      4081 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT
      4141 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC
      4201 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA
      4261 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA
      4321 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA
      4381 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA
      4441 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG
      4501 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
      4561 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
      4621 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
      4681 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
      4741 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
      4801 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA
      4861 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
      4921 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
      4981 AGTTTGCGCA ACGTTGTTGC CATTGCTGCA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
      5041 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
      5101 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
      5161 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
      5221 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
      5281 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
      5341 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
      5401 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
      5461 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
      5521 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAAAT TATTGAAGCA
      5581 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC
      5641 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TAAATTGTAA GCGTTAATGT
      5701 GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC
      5761 CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG
      5821 GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG
      5881 CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC CCATTCGCCA
//