LOCUS pMCSG73 6227 bp ds-DNA circular 26-SEP-2012 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers tRNA complement(2378..2529) /note="ileX gene" /label=ileX tRNA rep_origin complement(4220..4808) /note="pBR322 origin of replication" /label=pBR322 origin of replication promoter complement(1779..1795) /note="T7 Promoter" /label=T7 Promoter terminator complement(26..72) /note="T7 Terminator" /label=T7 Terminator CDS 2832..3791 /note="Lac I cds" /label=Lac I cds CDS complement(4979..5839) /note="bla cds" /label=bla cds tRNA complement(1862..2218) /note="argU tRNA" /label=argU tRNA misc_feature complement(218..238) /label=TVMV misc_feature complement(249..1708) /label=NusA misc_feature complement(200..217) /label=His6 misc_feature complement(176..199) /label=Strep II misc_feature complement(155..175) /label=TEV misc_feature complement(138..166) /label=LIC site ORIGIN 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC Tttcgggctt 121 tgttagcagc cggatGTTAt ccacttccaa tattggattg gaagtacagg ttctcCTTCT 181 CGAACTGTGG ATGAGACCAg tgatgatgat ggtggtgaga ctggaaacgc acggtttcgt 241 tgttgttatt acgggcagcc ataatcagtg ctccggcttt ttcgtcggtc aacccttcga 301 tatcagccag atcatcaatg ccctgttcgg cgagatcttc cagcgtacaa acgccacggg 361 cggccagttt gaatgccaaa tcacgatcta ccccttcaag gttcagcaga tcgtcagccg 421 gtttgttatc accgaggctt tcttcctggg cctgtgcaat ggtggccagt gcatttttag 481 cacgctcgcg cagtgcttca acggtcggct catcaaggcc ttcgatttcc aacagctctt 541 tcatcggcac ataggccaat tcttccagcg tcgagaagcc ttcttctacc agaacagtcg 601 cgaagtcttc gtcgatgtcg agatatttgg tgaaggtgtc gatcgctgcg tgcgcttccg 661 cctgatgctt agcttgcagg tcgtcaacgg tcatcacgtt gagttcccaa ccgctcagtt 721 gcgaagccag acgcacgttc tgaccgttac ggccaatcgc ctgcgccaga ttaccggctt 781 caacggcgat gtccatggtg tgtttatctt catccaccac gatagaagca acgtctgccg 841 gtgccattgc gttaatcacg aactgcgccg ggttatcatc ccacaggacg atatcgatac 901 gctcgccacc cagttcagta gacaccgcct gaacacgcgc gccacgcata cctacgcaag 961 cacctaccgg atcgatacgt ttatcgttgg ttttcaccgc gattttcgca cgagaacccg 1021 gatcgcgagc cgctgcttta atttcaatca cttcttcgcc gatttctggc acttcaatac 1081 ggaacagttc gatcagcatt tccggcttgg aacgagtgac gaacagttgc gcgccacgcg 1141 cttccgggcg aacggaatag agcacgccac gaacgcggtc gccagggcgg aagttttcac 1201 gcggcagcat atcttcgcgc aggatcacgg cttcagcgtt gttgcccaga tccagagaga 1261 tgttgtcgcg gtttactttt ttcaccacgc cggtgatgat ttcaccttcg tgttcacgga 1321 actgatcaac caccatcgca cgttcggctt cacgcacttt ctgcacgata acctgttttg 1381 ccgtctgggt agtgatacgg tcaaaggtaa cagactcaat ctgatcttca acgtaatcgc 1441 ccaggttcag gctttcatct tcataacgtg cggcttcaag ggtgatttcc ttggtcggct 1501 gggtgacttc atcaacaact aaccagcgac ggaaagtgtc aaaatcaccg cttttgcgat 1561 cgatctgtac gcggacgtcg atctcttgtt catatttttt ctttgttgct gtcgccagcg 1621 cgctttccaa tgcttcgaaa atcttctcgc gaggtagcgc cttttcattg gatacggctt 1681 caactacagc caaaatttct ttgttcatat gtatatctcc ttcttaaagt taaacaaaat 1741 tattctAGAG GGGAATTGTT ATCCGCTCAC AATTCCCCTA TAGTGAGTCG TATTAATTTC 1801 GCGGGATCGA GATCGATCTC GATCCTCTAC GCCGGACGCA TCGTGGCCGG CATCACCGGT 1861 GGATTCAGTC AGGCGTCCCA TTATCAGTGC TTCAGGAAAT GGGCGGATGG GAGTCCATAG 1921 AAATGGTTCG TAGGTATGCT CACCTTGCGC CTAATCATTT GACAGAGCAT GCGAGGAAAA 1981 TAGACGACAT TTTTGGTGAT AATGTCCCAA ATATGTCCCA CTCTGGAATT ATGGAGGATA 2041 TAAAGAAGGC GTAACTGATT GAATTGTAAT GGCGCGCCCT GCAGGATTCG AACCTGCGGC 2101 CCACGACTTA GAAGGTCGTT GCTCTATCCA ACTGAGCTAA GGGCGCGTTG ATACCGCAAT 2161 GCGGTGTAAT CGCGTGAATT ATACGGTCAA CCCTTGCTGA GTCAATGGCT TTTGATCTCG 2221 CCGGCGCCAC AGGTGCGGTT GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC 2281 GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT GGGTATGGTG GCAGGCCCCG 2341 TGGCCGGGGG ACTGTTGGGC GCCATCTCCT TGCATGCAAA AAGGCTGACG ATTTCTCGTC 2401 AGCCTTTGCT ATATCTGGTG GCCCCTGCTG GACTTGAACC AGCGACCAAG CGATTATGAG 2461 TCGCCTGCTC TAACCACTGA GCTAAGGGGC CATGGTAGCG GATTATAAAG TAACTCCGTG 2521 TCGCAATCCG CATGCACCAT TCCTTGCGGC GGCGGTGCTC AACGGCCTCA ACCTACTACT 2581 GGGCTGCTTC CTAATGCAGG AGTCGCATAA GGGAGAGCGT CGAGATCCCG GACACCATCG 2641 AATGGCGCAA AACCTTTCGC GGTATGGCAT GATAGCGCCC GGAAGAGAGT CAATTCAGGG 2701 TGGTGAATGT GAAACCAGTA ACGTTATACG ATGTCGCAGA GTATGCCGGT GTCTCTTATC 2761 AGACCGTTTC CCGCGTGGTG AACCAGGCCA GCCACGTTTC TGCGAAAACG CGGGAAAAAG 2821 TGGAAGCGGC GATGGCGGAG CTGAATTACA TTCCCAACCG CGTGGCACAA CAACTGGCGG 2881 GCAAACAGTC GTTGCTGATT GGCGTTGCCA CCTCCAGTCT GGCCCTGCAC GCGCCGTCGC 2941 AAATTGTCGC GGCGATTAAA TCTCGCGCCG ATCAACTGGG TGCCAGCGTG GTGGTGTCGA 3001 TGGTAGAACG AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT GCACAATCTT CTCGCGCAAC 3061 GCGTCAGTGG GCTGATCATT AACTATCCGC TGGATGACCA GGATGCCATT GCTGTGGAAG 3121 CTGCCTGCAC TAATGTTCCG GCGTTATTTC TTGATGTCTC TGACCAGACA CCCATCAACA 3181 GTATTATTTT CTCCCATGAA GACGGTACGC GACTGGGCGT GGAGCATCTG GTCGCATTGG 3241 GTCACCAGCA AATCGCGCTG TTAGCGGGCC CATTAAGTTC TGTCTCGGCG CGTCTGCGTC 3301 TGGCTGGCTG GCATAAATAT CTCACTCGCA ATCAAATTCA GCCGATAGCG GAACGGGAAG 3361 GCGACTGGAG TGCCATGTCC GGTTTTCAAC AAACCATGCA AATGCTGAAT GAGGGCATCG 3421 TTCCCACTGC GATGCTGGTT GCCAACGATC AGATGGCGCT GGGCGCAATG CGCGCCATTA 3481 CCGAGTCCGG GCTGCGCGTT GGTGCGGATA TCTCGGTAGT GGGATACGAC GATACCGAAG 3541 ACAGCTCATG TTATATCCCG CCGTTAACCA CCATCAAACA GGATTTTCGC CTGCTGGGGC 3601 AAACCAGCGT GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG GGCAATCAGC 3661 TGTTGCCCGT CTCACTGGTG AAAAGAAAAA CCACCCTGGC GCCCAATACG CAAACCGCCT 3721 CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA 3781 GCGGGCAGTG AGCGCAACGC AATTAATGTA AGTTAGCTCA CTCATTAGGC ACCGGGATCT 3841 CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG 3901 ACTACAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG 3961 ATTGTACTGA GAGTGCACCA TATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA 4021 AATACCGCAT CAGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 4081 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 4141 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 4201 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 4261 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 4321 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 4381 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 4441 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 4501 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 4561 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 4621 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG 4681 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 4741 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 4801 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 4861 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 4921 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 4981 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 5041 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 5101 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 5161 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 5221 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 5281 TTGTTGCCAT TGCTGCAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 5341 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 5401 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 5461 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 5521 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 5581 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 5641 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 5701 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 5761 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 5821 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAAATTAT TGAAGCATTT ATCAGGGTTA 5881 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 5941 GCGCACATTT CCCCGAAAAG TGCCACCTAA ATTGTAAGCG TTAATGTGAA CCATCACCCT 6001 AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC 6061 CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG 6121 CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA 6181 CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCA //