Cloning Vectors Used by MCSG

 

For high-throughput cloning the following vectors have been generated by MCSG:


 

Vector Map Genbank file PDF Linear map
pMCentr2 pMCentr2.gb pMCentr2.pdf
pMCentr3 pMCentr3.gb pMCentr3.pdf
pMCSG7 pMCSG7.gb pMCSG7.pdf
pMCSG8 pMCSG8.gb pMCSG8.pdf
pMCSG9 pMCSG9.gb pMCSG9.pdf
pMCSG10 pMCSG10.gb pMCSG10.pdf
pMCSG11 pMCSG11.gb pMCSG11.pdf
pMCSG12 pMCSG12.gb pMCSG12.pdf
pMCSG13 pMCSG13.gb pMCSG13.pdf
pMCSG14 pMCSG14.gb pMCSG14.pdf
pMCSG17 pMCSG17.gb pMCSG17.pdf
pMCSG18 pMCSG18.gb pMCSG18.pdf
pMCSG19 pMCSG19.gb pMCSG19.pdf
pMCSG19B pMCSG19B.gb pMCSG19B.pdf
pMCSG19C pMCSG19C.gb pMCSG19C.pdf
pMCSG20 pMCSG20.gb pMCSG20.pdf
pMCSG21 pMCSG21.gb pMCSG21.pdf
pMCSG22 pMCSG22.gb pMCSG22.pdf
pMCSG23 pMCSG23.gb pMCSG23.pdf
pMCSG24 pMCSG24.gb pMCSG24.pdf
pMCSG25 pMCSG25.gb pMCSG25.pdf
pMCSG26 pMCSG26.gb pMCSG26.pdf
pMCSG26doc.pdf
pMCSG27 pMCSG27.gb pMCSG27.pdf
pMCSG27doc.pdf
pMCSG28 pMCSG28.gb pMCSG28.pdf
pMCSG28doc.pdf
pMCSG29 pMCSG29.gb pMCSG29.pdf
pMCSG29doc.pdf
pMCSG30 pMCSG30.gb pMCSG30.pdf
pMCSG31 pMCSG31.gb pMCSG31.pdf
pMCSG32 pMCSG32.gb pMCSG32.pdf
pMCSG33 pMCSG33.gb pMCSG33.pdf
pMCSG34 pMCSG34.gb pMCSG34.pdf
pMCSG35 pMCSG35.gb pMCSG35.pdf
pMCSG36 pMCSG36.gb pMCSG36.pdf
pMCSG37 pMCSG37.gb pMCSG37.pdf
pMCSG38 pMCSG38.gb pMCSG38.pdf
pMCSG39 pMCSG39.gb pMCSG39.pdf
pMCSG40 pMCSG40.gb pMCSG40.pdf
pMCSG41 pMCSG41.gb pMCSG41.pdf
pMCSG42 pMCSG42.gb pMCSG42.pdf
pMCSG43 pMCSG43.gb pMCSG43.pdf
pMCSG46 pMCSG46.gb pMCSG46.pdf
pMCSG48 pMCSG48.gb pMCSG48.pdf
pMCSG49 pMCSG49.gb pMCSG49.pdf
pMCSG50 pMCSG50.gb pMCSG50.pdf
pMCSG51 pMCSG51.gb pMCSG51.pdf
pMCSG52 pMCSG52.gb pMCSG52.pdf
pMCSG53 pMCSG53.gb pMCSG53.pdf
pMCSG54 pMCSG54.gb pMCSG54.pdf
pMCSG55 pMCSG55.gb pMCSG55.pdf
pMCSG56 pMCSG56.gb pMCSG56.pdf
pMCSG57 pMCSG57.gb pMCSG57.pdf
pMCSG58 pMCSG58.gb pMCSG58.pdf
pMCSG59 pMCSG59.gb pMCSG59.pdf
pMCSG60 pMCSG60.gb pMCSG60.pdf
pMCSG61 pMCSG61.gb pMCSG61.pdf
pMCSG62 pMCSG62.gb pMCSG62.pdf
pMCSG63 pMCSG63.gb pMCSG63.pdf
pMCSG64 pMCSG64.gb pMCSG64.pdf
pMCSG65 pMCSG65.gb pMCSG65.pdf
pMCSG66 pMCSG66.gb pMCSG66.pdf
pMCSG67 pMCSG67.gb pMCSG67.pdf
pMCSG68 pMCSG68.gb pMCSG68.pdf
pMCSG69 pMCSG69.gb pMCSG69.pdf
pMCSG70 pMCSG70.gb pMCSG70.pdf
pMCSG71 pMCSG71.gb pMCSG71.pdf
pMCSG73 pMCSG73.gb pMCSG73.pdf
pMCSG73.02.pdf
pMCSG73.03.pdf
pMCSG79 pMCSG79.gb pMCSG79.pdf
pMCSG79.02.pdf
pMCSG81 pMCSG81.gb pMCSG81.pdf
pMCSG81.desc.pdf

These vectors are available from DNASU: http://dnasu.asu.edu/DNASU/GetAllVectors.do?PSI=true.

The vector summary is availble in PDF format.

name base antibi tag&sites
pET30Xa/LIC pET30a kan N-His-Thrmb-S-Xa-LICb-MCS-His-C
(pET21a pET amp N-T7tag-MCS-His-C
pMCSG1 pET30Xa/LIC kan N-His-Xa-LICb-MCS-His-C
pMCSG3 pET21a amp N-His-Xa-LICb-MCS-His-C
pMCSG6 pMCSG3 amp N-His-TEV-LICb-MCS-His-C
pMCSG7 pMCSG6 amp N-His-TEV-LIC-MCS-His-C
pMCSG8 pMCSG7 amp N-His-Sloop-TEV-LIC-MCS-His-C
pMCSG9 pMCSG7 amp N-His-MBP-TEV-LIC-MCS-His-C
pMCSG10 pMCSG7 amp N-His-GST-TEV-LIC-MCS-His-C
pMCSG11 pACYCDuet Cm N-His-TEV-LIC-MCS-His-C
pMCSG12 pACYCDuet Cm N-His-Sloop-TEV-LIC-MCS-His-C
pMCSG13 pACYCDuet Cm N-His-MBP-TEV-LIC-MCS-His-C
pMCSG14 pACYCDuet Cm N-His-GST-TEV-LIC-MCS-His-C
pMCSG15 pMCSG7 amp N-LIC-GS-TEV-AviTag-His-C
pMCSG16 pMCSG7 amp N-His-AviTag-GS-TEV-LIC-MCS-His-C
pMCSG17 pMCSG7 amp N-Stag-TEV-LIC-MCS-His-C
pMCSG18 pMCSG7 amp N-His-TEV-LIC-GFP-MCS-His-C
pMCSG19 pMCSG7 amp N-MBP-TVMV-His-TEV-LIC-MCS-His-C
pMCSG19B pMCSG7 amp N-MBP-TVMV-His-TEV-LIC-MCS-His-C TVMV
pMCSG19C pMCSG7 amp N-MBP-TVMV-His-TEV-LIC-MCS-His-C TVMV
pMCSG20 pMCSG17 amp N-Stag-GST-TEV-LIC-MCS-His-C
pMCSG21 pCDFDuet spec N-His-TEV-LIC-MCS-His-C
pMCSG22 pMCSG21 spec N-His-Sloop-TEV-LIC-MCS-His-C
pMCSG23 pMCSG21 spec N-His-MBP-TEV-LIC-MCS-His-C
pMCSG24 pMCSG21 spec N-His-GST-TEV-LIC-MCS-His-C
pMCSG25 pMCSG7 amp N-Protease-His-TEV-LIC-MCS-His-C
pMCSG26 pMCSG7 amp N-LIC3-His6-C
pMCSG27 pMCSG26 amp N-LIC3-His10-TVMV-MBP-C
pMCSG28 pMCSG26 amp N-LIC4-TEV-His6-C
pMCSG29 pMCSG27 amp N-LIC4-TEV-His10-TVMV-MBP-C
pMCSG30 pMCSG7 amp N-His-TEV-MPB-LIC2-SacB-LIC2-C
pMCSG31 pASKIBA3+ amp N-MBP-TVMV-His6-TEV-LIC (Tet-inducible TVMV)
pMCSG32 pMCSG28 amp N-MBP-TVMV-LIC-TEV-His6-C
pMCSG33 pMCSG28 amp N-His10-MBP-TEV-LIC-His6-C
pMCSG34 pMCSG32 amp N-MBP-TVMV-LIC-TEV-His6-C (TVMV)
pMCSG34B pMCSG32 amp N-MBP-TVMV-LIC-TEV-His6-C (TVMV anti-clock)
pMCSG35 pMCSG28 amp N-His10-MBP-TEV-LIC-His6-C (TVMV)
pMCSG35b pMCSG28 amp N-His10-MBP-TEV-LIC-His6-C (TVMV anti-clock)
pMCSG37 pMCSG9 amp N-His10-MBP-His6-TEV-LIC-C (TVMV)
pMCSG38 pMCSG7 amp N-His6-TEV-LIC-C (TVMV anti-clock)
pMCSG38C pMCSG7 amp N-His6-TEV-LIC-C (TVMV)
pMCSG39 pMCSG7 amp N-MBP-TVMV-His10-TEV-LIC-C
pMCSG40 pMCSG19 amp N-OsmY-TVMV-His10-TEV-LIC-C (DsbA)
pMCSG41 pMCSG28 amp N-LIC-TEV-His6-C (TVMV)
pMCSG42 pMCSG32 amp N-MBP-TVMV-LIC-TEV-His6-C (TVMV)
pMCSG43 pMCSG32 amp N-OsmY-TVMV-LIC-TEV-His6-C (DsbA)
pMCSG44 pMCSG7 amp N-HN6-SUMO-TEV-LIC-C
pMCSG45 pMCSG7 amp N-HN6-MBP-TEV-LIC-C
pMCSG46 pMCSG7 amp N-HN6-NusA-TEV-LIC-C
pMCSG47 pMCSG7 amp N-HN6-Halo-TEV-LIC-C
pMCSG48 pMCSG7 amp N-His8-NusA-TEV-LIC-C
pMCSG49 pMCSG7 amp N-His-TEV-LIC-C (1kb removed))
pMCSG50 pMCSG60 amp N-His6-AviTag-TEV-LICs-BirA-C
pMCSG51 pMCSG50 amp N-His6-AviTag-TEV-LICs-BirA-C
pMCSG52 pMCSG7 amp N-His-TEV-LIC-C (Rare: argU-ileX)
pMCSG53 pMCSG52 amp N-His-TEV-LIC-C (Rare: argU-ileX; 1kb removed)
pMCSG54 pMCSG49 amp N-His-FLAG-TEV-LIC-C
pMCSG55 pMCSG49 amp N-His-HA-TEV-LIC-C
pMCSG56 pMCSG49 amp N-His-HA-HA-TEV-LIC-C
pMCSG57 pMCSG49 amp N-His-STRII-TEV-LIC-C
pMCSG58 pMCSG53 amp N-LIC-His-C  (Rare: argU-ileX; 1kb removed)
pMCSG59 pMCSG53 amp N-LIC-TEV-His-C  (Rare: argU-ileX; 1kb removed)
pMCSG60 pMCSG7 amp N-His6-TEV-LIC-LIC4-C
pMCSG61 pMCSG30 amp N-His6-TEV-MBP-LIC-C
pMCSG62 pMCSG53 amp N-His6-AviTag-TEV-LIC-BirA-C  (Rare: argU-ileX; 1kb removed)
pMCSG63 pMCSG53 amp N-His6-TEV-LIC-LIC-C (Rare: argU-ileX; 1kb removed)
pMCSG64 pMCSG60 amp N-His-FLAG-TEV-LIC-LIC4-C
pMCSG65 pMCSG60 amp N-His-HA-TEV-LIC-LIC4-C
pMCSG66 pMCSG60 amp N-His-HA-HA-TEV-LIC-LIC4-C
pMCSG67 pMCSG60 amp N-His-STRII-TEV-LIC-LIC4-C
pMCSG68 pMCSG53 amp N-His-STRII-TEV-LIC-C  (Rare: argU-ileX; 1kb removed)
pMCSG69 pMCSG53 amp N-MBP-TVMV-His-TEV-LIC-C  (Rare: argU-ileX; 1kb removed)
pMCSG70 pMCSG53 amp N-LIC-TEV-His10-TVMV-MBP-C   (Rare: argU-ileX; 1kb removed)
pMCSG71 pMCSG53 amp N-MBP-TVMV-LIC-TEV-His-C  (Rare: argU-ileX; 1kb removed)
pMCSG72 pMCSG53 N-NusA-TVMV-His6-TEV-LIC-C  (Rare: argU-ileX; 1kb removed
pMCSG73 pMCSG53 amp N-NusA-TVMV-His6-STRII-TEV-LIC-C  (Rare: argU-ileX; 1kb removed)
pMCSG74 pMCSG53 amp N-NusA-TVMV-STRII-His6-TEV-LIC  (Rare: argU-ileX; 1kb removed)
pMCSG75 pMCSG62 amp N-LIC-TEV-biotin-His6-BirA-C  (Rare: argU-ileX; 1kb removed)
pMCSG76 pMCSG21 spec N-His6-TEV-LIC1-LIC2-C  (Rare: argU-ileX; 1kb removed)
pMCSG77 pMAGIC kan N-His6-TEV-LIC1-LIC2-C  (Rare: argU-ileX; 1kb removed)
pMCSG78 pMCSG53 amp N-His-TVMV-LIC-C  (Rare: argU-ileX; 1kb removed)
pMCSG79 pFastBac amp N-His6-TEV-LIC-C (LIC between BamHI and XbaI - ColE1; Gentamycin)
pMCSG80 pMCSG80 amp
pMCSG81 pMCSG53 amp N-PIPE-His6-C (Rare: argU-ileX; 1kb removed)
pMCentr1 pDONR/Zeo zeocin attL1-TEV-LIC-attL2
pMCentr2 pDONR221 kan attL1-TEV-LIC-attL2
pMCentr3 pDONR221 kan attL1-TVMV-LIC-attL2
pMCdest19 pMCSG19 amp, Cm attR1-ccdB-CmR-attR2


Full listing of vectors and their features

 

 

 Selected related publications:

 

Brett TJ, Legendre-Guillemin V, McPherson PS, Fremont DH (2006)
Structural definition of the F-actin-binding THATCH domain from HIP1R.
Nat Struct Mol Biol
, 13, 121-30 Times cited: 6. [PubMed] [PDB]

Dieckman L, Gu M, Stols L, Donnelly MI, Collart FR (2002)
High throughput methods for gene cloning and expression.
Protein Expr Purif
, 25, 1-7 Times cited: 42. [PubMed]

Donnelly MI, Stevens PW, Stols L, Su SX, Tollaksen S, Giometti C, Joachimiak A (2001)
Expression of a highly toxic protein, Bax, in Escherichia coli by attachment of a leader
peptide derived from the GroES cochaperone. Protein Expr Purif, 22, 422-9 Times cited: 7. [PubMed]

Donnelly MI, Zhou M, Millard CS, Clancy S, Stols L, Eschenfeldt WH, Collart FR, Joachimiak A (2006)
An expression vector tailored for large-scale, high-throughput purification of recombinant proteins.
Protein Expr Purif, 47, 446-54 Times cited: 5. [PubMed]

Moy S, Dieckman L, Schiffer M, Maltsev N, Yu GX, Collart FR (2004)
Genome-scale expression of proteins from Bacillus subtilis.
J Struct Funct Genomics, 5, 103-9 Times cited: no data. [PubMed]

Nelson CA, Pekosz A, Lee CA, Diamond MS, Fremont DH (2005)
Structure and intracellular targeting of the SARS-coronavirus Orf7a accessory protein.
Structure (Camb)
, 13, 75-85 Times cited: 21. [PubMed] [PDB]

Scholle MD, Collart FR, Kay BK (2004)
In vivo biotinylated proteins as targets for phage-display selection experiments.
Protein Expr Purif, 37, 243-52 Times cited: 6. [PubMed]

Smith HR, Heusel JW, Mehta IK, Kim S, Dorner BG, Naidenko OV, Iizuka K, Furukawa H,
Beckman DL, Pingel JT, Scalzo AA, Fremont DH, Yokoyama WM (2002) 
Recognition of a virus-encoded ligand by a natural killer cell activation receptor.
Proc Natl Acad Sci U S A
, 99, 8826-31 Times cited: 181. [PubMed]

Stevens FJ, Kuemmel C, Babnigg G, Collart FR (2004)
Efficient recognition of protein fold at low sequence identity by conservative application
of Psi-BLAST: application. J Mol Recognit, 18, 150-157 Times cited: 2. [PubMed]

Stols L, Gu M, Dieckman L, Raffen R, Collart FR, Donnelly MI (2002)
A new vector for high-throughput, ligation-independent cloning encoding
a tobacco etch virus protease cleavage site. Protein Expr Purif, 25, 8-15 Times cited: 57. [PubMed]

Stols L, Millard CS, Dementieva I, Donnelly MI (2004)
Production of selenomethionine-labeled proteins in two-liter plastic bottles
for structure determination. J Struct Funct Genomics, 5, 95-102 Times cited: no data. [PubMed]

Stols L, Zhou M, Eschenfeldt WH, Millard CS, Abdullah J, Collart FR, Kim Y, Donnelly MI (2007)
New vectors for co-expression of proteins: Structure of Bacillus subtilis ScoAB obtained by
high-throughput protocols. Protein Expr Purif, 53, 396-403 Times cited: 0. [PubMed]

Yoon JR, Laible PD, Gu M, Scott HN, Collart FR (2002)
Express primer tool for high-throughput gene cloning and expression.
Biotechniques
, 33, 1328-33 Times cited: 3. [PubMed]

...

For a more exhaustive list of publications see the MCSG publications website.