FEATURES             Location/Qualifiers
     rep_origin      2496..3408
                     /label="P15A\ori
     CDS             complement(3532..4614)
                     /label="lacI
     promoter        4738..8833
                     /label="T7\Promoter
     protein_bind    5..25
                     /label="lacO
     CDS             complement(1475..2134)
                     /label="CAT
     RBS             58..63
                     /label="RBS-1
     Region          83..100
                     /label="His-Tag
     terminator      1208..1255
                     /label="T7\Terminator
     Region          1112..1156
                     /label="S-Tag
     protein_bind    235..255
                     /label="lacO
     promoter        214..230
                     /label="T7\promoter
     RBS             286..291
                     /label="RBS
     primer          4695..4714
                     /label="ACYCDuetUP1\Primer\#71178-3
     primer          189..208
                     /label="DuetUP2\Primer\#71180-3
     primer          complement(189..208)
                     /label="DuetDOWN1\Primer\#71179-3
     primer          complement(1194..1212)
                     /label="T7\Terminator\Primer\#69337-3
     misc_feature    297..1105
                     /note="pMCSG10"
     CDS             300..1058
                     /note="coding region"
     Region          978..995
                     /note="His Tag"
     Cleavage-site   1020..1040
                     /note="TEV Site"
     misc_feature    1028..1059
                     /note="LIC Site"
     Region          339..998
                     /note="GST;GST"

         1 GGGGAATTGT GAGCGGATAA CAATTCCCCT GTAGAAATAA TTTTGTTTAA CTTTAATAAG
        61 GAGATATACC ATGGGCAGCA GCCATCACCA TCATCACCAC AGCCAGGATC CGAATTCGAG
       121 CTCGGCGCGC CTGCAGGTCG ACAAGCTTGC GGCCGCATAA TGCTTAAGTC GAACAGAAAG
       181 TAATCGTATT GTACACGGCC GCATAATCGA AATTAATACG ACTCACTATA GGGGAATTGT
       241 GAGCGGATAA CAATTCCCCA TCTTAGTATA TTAGTTAAGT ATAAGAAGGA GATATACATA
       301 TGCACCATCA TCATCATCAT TCTTCTGGTG TAGATCTGAT GTCCCCTATA CTAGGTTATT
       361 GGAAAATTAA GGGCCTTGTG CAACCCACTC GACTTCTTTT GGAATATCTT GAAGAAAAAT
       421 ATGAAGAGCA TTTGTATGAG CGCGATGAAG GTGATAAATG GCGAAACAAA AAGTTTGAAT
       481 TGGGTTTGGA GTTTCCCAAT CTTCCTTATT ATATTGATGG TGATGTTAAA TTAACACAGT
       541 CTATGGCCAT CATACGTTAT ATAGCTGACA AGCACAACAT GTTGGGTGGT TGTCCAAAAG
       601 AGCGTGCAGA GATTTCAATG CTTGAAGGAG CGGTTTTGGA TATTAGATAC GGTGTTTCGA
       661 GAATTGCATA TAGTAAAGAC TTTGAAACTC TCAAAGTTGA TTTTCTTAGC AAGCTACCTG
       721 AAATGCTGAA AATGTTCGAA GATCGTTTAT GTCATAAAAC ATATTTAAAT GGTGATCATG
       781 TAACCCATCC TGACTTCATG TTGTATGACG CTCTTGATGT TGTTTTATAC ATGGACCCAA
       841 TGTGCCTGGA TGCGTTCCCA AAATTAGTTT GTTTTAAAAA ACGTATTGAA GCTATCCCAC
       901 AAATTGATAA GTACTTGAAA TCCAGCAAGT ATATAGCATG GCCTTTGCAG GGCTGGCAAG
       961 CCACGTTTGG TGGTGGCGAC CATCCTCCAA AATCGGATGA TTACCCAGAT CTGGGTACCG
      1021 AGAACCTGTA CTTCCAATCC AATATTGGAA GTGGATAACG GATCCGAATT CGAGCTCCGT
      1081 CGACAAGCTT GCGGCCGCAC TCGAGTCTGG TAAAGAAACC GCTGCTGCGA AATTTGAACG
      1141 CCAGCACATG GACTCGTCTA CTAGCGCAGC TTAATTAACC TAGGCTGCTG CCACCGCTGA
      1201 GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA
      1261 ACCTCAGGCA TTTGAGAAGC ACACGGTCAC ACTGCTTCCG GTAGTCAATA AACCGGTAAA
      1321 CCAGCAATAG ACATAAGCGG CTATTTAACG ACCCTGCCCT GAACCGACGA CCGGGTCGAA
      1381 TTTGCTTTCG AATTTCTGCC ATTCATCCGC TTATTATCAC TTATTCAGGC GTAGCACCAG
      1441 GCGTTTAAGG GCACCAATAA CTGCCTTAAA AAAATTACGC CCCGCCCTGC CACTCATCGC
      1501 AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAG ACGGCATGAT
      1561 GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAGTAT TTGCCCATAG
      1621 TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA CTGGTGAAAC
      1681 TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT AAACCCTTTA GGGAAATAGG
      1741 CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT GTGTAGAAAC TGCCGGAAAT
      1801 CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT TTGCTCATGG AAAACGGTGT
      1861 AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC ATACGGAACT
      1921 CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT
      1981 TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG
      2041 TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT
      2101 CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC TTCCTTAGCT CCTGAAAATC
      2161 TCGATAACTC AAAAAATACG CCCGGTAGTG ATCTTATTTC ATTATGGTGA AAGTTGGAAC
      2221 CTCTTACGTG CCGATCAACG TCTCATTTTC GCCAAAAGTT GGCCCAGGGC TTCCCGGTAT
      2281 CAACAGGGAC ACCAGGATTT ATTTATTCTG CGAAGTGATC TTCCGTCACA GGTATTTATT
      2341 CGGCGCAAAG TGCGTCGGGT GATGCTGCCA ACTTACTGAT TTAGTGTATG ATGGTGTTTT
      2401 TGAGGTGCTC CAGTGGCTTC TGTTTCTATC AGCTGTCCCT CCTGTTCAGC TACTGACGGG
      2461 GTGGTGCGTA ACGGCAAAAG CACCGCCGGA CATCAGCGCT AGCGGAGTGT ATACTGGCTT
      2521 ACTATGTTGG CACTGATGAG GGTGTCAGTG AAGTGCTTCA TGTGGCAGGA GAAAAAAGGC
      2581 TGCACCGGTG CGTCAGCAGA ATATGTGATA CAGGATATAT TCCGCTTCCT CGCTCACTGA
      2641 CTCGCTACGC TCGGTCGTTC GACTGCGGCG AGCGGAAATG GCTTACGAAC GGGGCGGAGA
      2701 TTTCCTGGAA GATGCCAGGA AGATACTTAA CAGGGAAGTG AGAGGGCCGC GGCAAAGCCG
      2761 TTTTTCCATA GGCTCCGCCC CCCTGACAAG CATCACGAAA TCTGACGCTC AAATCAGTGG
      2821 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCTGGCGGC TCCCTCGTGC
      2881 GCTCTCCTGT TCCTGCCTTT CGGTTTACCG GTGTCATTCC GCTGTTATGG CCGCGTTTGT
      2941 CTCATTCCAC GCCTGACACT CAGTTCCGGG TAGGCAGTTC GCTCCAAGCT GGACTGTATG
      3001 CACGAACCCC CCGTTCAGTC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
      3061 AACCCGGAAA GACATGCAAA AGCACCACTG GCAGCAGCCA CTGGTAATTG ATTTAGAGGA
      3121 GTTAGTCTTG AAGTCATGCG CCGGTTAAGG CTAAACTGAA AGGACAAGTT TTGGTGACTG
      3181 CGCTCCTCCA AGCCAGTTAC CTCGGTTCAA AGAGTTGGTA GCTCAGAGAA CCTTCGAAAA
      3241 ACCGCCCTGC AAGGCGGTTT TTTCGTTTTC AGAGCAAGAG ATTACGCGCA GACCAAAACG
      3301 ATCTCAAGAA GATCATCTTA TTAATCAGAT AAATTATTTC TAGATTTCAG TGCAATTTAT
      3361 CTCTTCAAAT GTAGCACCTG AAGTCAGCCC CATACGATAT AAGTTGTAAT TCTCATGTTA
      3421 GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT
      3481 CGAGATCCCG GTGCCTAATG AGTGAGCTAA CTTACATTAA TTGCGTTGCG CTCACTGCCC
      3541 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
      3601 AGAGGCGGTT TGCGTATTGG GCGCCAGGGT GGTTTTTCTT TTCACCAGTG AGACGGGCAA
      3661 CAGCTGATTG CCCTTCACCG CCTGGCCCTG AGAGAGTTGC AGCAAGCGGT CCACGCTGGT
      3721 TTGCCCCAGC AGGCGAAAAT CCTGTTTGAT GGTGGTTAAC GGCGGGATAT AACATGAGCT
      3781 GTCTTCGGTA TCGTCGTATC CCACTACCGA GATGTCCGCA CCAACGCGCA GCCCGGACTC
      3841 GGTAATGGCG CGCATTGCGC CCAGCGCCAT CTGATCGTTG GCAACCAGCA TCGCAGTGGG
      3901 AACGATGCCC TCATTCAGCA TTTGCATGGT TTGTTGAAAA CCGGACATGG CACTCCAGTC
      3961 GCCTTCCCGT TCCGCTATCG GCTGAATTTG ATTGCGAGTG AGATATTTAT GCCAGCCAGC
      4021 CAGACGCAGA CGCGCCGAGA CAGAACTTAA TGGGCCCGCT AACAGCGCGA TTTGCTGGTG
      4081 ACCCAATGCG ACCAGATGCT CCACGCCCAG TCGCGTACCG TCTTCATGGG AGAAAATAAT
      4141 ACTGTTGATG GGTGTCTGGT CAGAGACATC AAGAAATAAC GCCGGAACAT TAGTGCAGGC
      4201 AGCTTCCACA GCAATGGCAT CCTGGTCATC CAGCGGATAG TTAATGATCA GCCCACTGAC
      4261 GCGTTGCGCG AGAAGATTGT GCACCGCCGC TTTACAGGCT TCGACGCCGC TTCGTTCTAC
      4321 CATCGACACC ACCACGCTGG CACCCAGTTG ATCGGCGCGA GATTTAATCG CCGCGACAAT
      4381 TTGCGACGGC GCGTGCAGGG CCAGACTGGA GGTGGCAACG CCAATCAGCA ACGACTGTTT
      4441 GCCCGCCAGT TGTTGTGCCA CGCGGTTGGG AATGTAATTC AGCTCCGCCA TCGCCGCTTC
      4501 CACTTTTTCC CGCGTTTTCG CAGAAACGTG GCTGGCCTGG TTCACCACGC GGGAAACGGT
      4561 CTGATAAGAG ACACCGGCAT ACTCTGCGAC ATCGTATAAC GTTACTGGTT TCACATTCAC
      4621 CACCCTGAAT TGACTCTCTT CCGGGCGCTA TCATGCCATA CCGCGAAAGG TTTTGCGCCA
      4681 TTCGATGGTG TCCGGGATCT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAATTAA
      4741 TACGACTCAC TATA
//