FEATURES             Location/Qualifiers
     CDS             complement(1881..2591)
                     /note="TVMV protease"
     promoter        complement(2610..2765)
                     /note="pLUX I - Tet 0"
     misc_feature    204..235
                     /note="LIC Site"
     Cleavage-site   223..243
                     /note="TEV Site"
     Region          268..285
                     /note="His Tag"
     CDS             complement(205..1443)
                     /note="coding region"
     promoter        complement(1515..1531)
                     /note="T7 Promoter"
     terminator      complement(26..72)
                     /note="T7 Terminator"
     CDS             3072..4031
                     /note="Lac I cds"
     CDS             complement(6220..7080)
                     /note="bla cds"
     rep_origin      5432..5432
                     /note="pBR322 origin of replication"
     Region          complement(337..1443)
                     /note="maltose binding protein"
     Cleavage-site   complement(286..306)
                     /note="TVMV cleavage site"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTATCC ACTTCCAATA TTGGATTGGA AGTACAGGTT
       241 CTCGGTACCC AGATCCACGC CAGAAGAGTG ATGATGATGG TGGTGAGACT GGAAACGCAC
       301 GGTTTCCGAG CCTGCTTTTT TGTACAAACT TGTGATCGAA TTAGTCTGCG CGTCTTTCAG
       361 GGCTTCATCG ACAGTCTGAC GACCGCTGGC GGCGTTGATC ACCGCAGTAC GCACGGCATA
       421 CCAGAAAGCG GACATCTGCG GGATGTTCGG CATGATTTCA CCTTTCTGGG CGTTTTCCAT
       481 GGTGGCGGCA ATACGTGGAT CTTTCGCCAA CTCTTCCTCG TAAGACTTCA GCGCTACGGC
       541 ACCCAGCGGT TTGTCTTTAT TAACCGCTTC CAGACCTTCA TCAGTCAGCA GATAGTTTTC
       601 GAGGAACTCT TTTGCCAGCT CTTTGTTCGG ACTGGCGGCG TTAATACCTG CGCTCAGCAC
       661 GCCAACGAAC GGTTTGGATG GTTGACCCTT GAAGGTCGGC AGTACCGTTA CACCATAATT
       721 CACTTTGCTG GTGTCGATGT TGGACCATGC CCACGGGCCG TTGATGGTCA TCGCTGTTTC
       781 GCCTTTATTA AAGGCAGCTT CTGCGATGGA GTAATCGGTG TCTGCATTCA TGTGTTTGTT
       841 TTTAATCAGG TCAACCAGGA AGGTCAGACC CGCTTTCGCG CCAGCGTTAT CCACGCCCAC
       901 GTCTTTAATG TCGTACTTGC CGTTTTCATA CTTGAACGCA TAACCCCCGT CAGCAGCAAT
       961 CAGCGGCCAG GTGAAGTACG GTTCTTGCAG GTTGAACATC AGCGCGCTCT TACCTTTCGC
      1021 TTTCAGTTCT TTATCCAGCG CCGGGATCTC TTCCCAGGTT TTTGGCGGGT TCGGCAGCAG
      1081 ATCTTTGTTA TAAATCAGCG ATAACGCTTC AACAGCGATC GGGTAAGCAA TCAGCTTGCC
      1141 GTTGTAACGT ACGGCATCCC AGGTAAACGG ATACAGCTTG TCCTGGAACG CTTTGTCCGG
      1201 GGTGATTTCA GCCAACAGGC CAGATTGAGC GTAGCCACCA AAGCGGTCGT GTGCCCAGAA
      1261 GATAATGTCA GGGCCATCGC CAGTTGCCGC AACCTGTGGG AATTTCTCTT CCAGTTTATC
      1321 CGGATGCTCA ACGGTGACTT TAATTCCGGT ATCTTTCTCG AATTTCTTAC CGACTTCAGC
      1381 GAGACCGTTA TAGCCTTTAT CGCCGTTAAT CCAGATTACC AGTTTACCTT CTTCGATTTT
      1441 CATATGTATA TCTCCTTCTT AAAGTTAAAC AAAATTATTT CTAGAGGGGA ATTGTTATCC
      1501 GCTCACAATT CCCCTATAGT GAGTCGTATT AATTTCGCGG GATCGAGATC GATCTCGATC
      1561 CTCTACGCCG GACGCATCGT GGCCGGCATC ACCGGCGCCA CAGGTGCGGT TGCTGGCGCC
      1621 TATATCGCCG ACATCACCGA TGGGGAAGAT CGGGCTCGCC ACTTCGGGCT CATGAGCGCT
      1681 TGTTTCGGCG TGGGTATGGT GGCAGGCCCC GTGGCCGGGG GACTGTTGGG CGCCATCTCC
      1741 TTGCATGCTC TAGGGCGGCG GATTTGTCCT ACTCAGGAGA GCGTTCACCG ACAAACAACA
      1801 GATAAAACGA AAGGCCCAGT CTTTCGACTG AGCCTTTCGT TTTATTTGAT GCCTCTAGCA
      1861 CGCGTACCAT GGGATCTTTA TTAGTCCATG ATGGCGGCAA CAGTTTTTTT GGCCATGAAG
      1921 TCATCTTCCG GCGCATCTTC AACCAGGGTA AAGGAACCCC AGCTGATTTT ATCCGCGTTG
      1981 AATTTCCAGT TTTTGCACCA ACCATCCGCG GCATCTAGAT AAGTCGCCAC GAATTTTTCC
      2041 GGAAATTCCA CGAAGTAGTT GCTACCGTTG GTGGTATGAG TCAGGCTGTG GATGCCCAGA
      2101 ATGTTGCCAT CAATGATGGA AACTAGTGGG CTGCCACACT GGCCATCTTT AGTGGTGATC
      2161 CAGTGCTGCC AGAAAGAAGT GTCTTCTTTA TGCACAATGT GAGAGGATTC AGACACCAGG
      2221 CTCGAGACGC TTTTCTGCTG AAAGTTGGTG GACACCATGC ACACACGATC TTTGATGGTC
      2281 GGCTGACGGA ATTTCAGTTT CTGCGGGAAC GGCGGGAAGT CTTTAGCCAT TTTGATAACG
      2341 ATAATGTCAC GGCCTTCAAC CGGTTTCATC TGCAGCTGGG TAGAGTTTTT GACTTTGAAT
      2401 TCACCATGCA TGGTTTTGAT GGTCAGTTCG CCATTGTTAC GACGAAACAG ATGCTGGTTG
      2461 GCAATGATAT ACGGGCCAAA ACCAATGCCA AACAGACGTT CACTATGACC ATCCGAGGAG
      2521 TTTTCCAGCA GGCATACGCA AGCAGAGATC GGATTAAAAT CGCGCACGCC CTTCAGCAAA
      2581 GCTTTAGACA TATGTTACCT CCTTAAATCA ATTCGGTCAG TGCGTCCTGC TGATGTGCTC
      2641 AGTATCTCTA TCACTGATAG GGATGTCAAT CTCTATCACT GATAGGGACT CGAGGTGAAG
      2701 ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC
      2761 TTAGACGTCG CATGCACCAT TCCTTGCGGC GGCGGTGCTC AACGGCCTCA ACCTACTACT
      2821 GGGCTGCTTC CTAATGCAGG AGTCGCATAA GGGAGAGCGT CGAGATCCCG GACACCATCG
      2881 AATGGCGCAA AACCTTTCGC GGTATGGCAT GATAGCGCCC GGAAGAGAGT CAATTCAGGG
      2941 TGGTGAATGT GAAACCAGTA ACGTTATACG ATGTCGCAGA GTATGCCGGT GTCTCTTATC
      3001 AGACCGTTTC CCGCGTGGTG AACCAGGCCA GCCACGTTTC TGCGAAAACG CGGGAAAAAG
      3061 TGGAAGCGGC GATGGCGGAG CTGAATTACA TTCCCAACCG CGTGGCACAA CAACTGGCGG
      3121 GCAAACAGTC GTTGCTGATT GGCGTTGCCA CCTCCAGTCT GGCCCTGCAC GCGCCGTCGC
      3181 AAATTGTCGC GGCGATTAAA TCTCGCGCCG ATCAACTGGG TGCCAGCGTG GTGGTGTCGA
      3241 TGGTAGAACG AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT GCACAATCTT CTCGCGCAAC
      3301 GCGTCAGTGG GCTGATCATT AACTATCCGC TGGATGACCA GGATGCCATT GCTGTGGAAG
      3361 CTGCCTGCAC TAATGTTCCG GCGTTATTTC TTGATGTCTC TGACCAGACA CCCATCAACA
      3421 GTATTATTTT CTCCCATGAA GACGGTACGC GACTGGGCGT GGAGCATCTG GTCGCATTGG
      3481 GTCACCAGCA AATCGCGCTG TTAGCGGGCC CATTAAGTTC TGTCTCGGCG CGTCTGCGTC
      3541 TGGCTGGCTG GCATAAATAT CTCACTCGCA ATCAAATTCA GCCGATAGCG GAACGGGAAG
      3601 GCGACTGGAG TGCCATGTCC GGTTTTCAAC AAACCATGCA AATGCTGAAT GAGGGCATCG
      3661 TTCCCACTGC GATGCTGGTT GCCAACGATC AGATGGCGCT GGGCGCAATG CGCGCCATTA
      3721 CCGAGTCCGG GCTGCGCGTT GGTGCGGATA TCTCGGTAGT GGGATACGAC GATACCGAAG
      3781 ACAGCTCATG TTATATCCCG CCGTTAACCA CCATCAAACA GGATTTTCGC CTGCTGGGGC
      3841 AAACCAGCGT GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG GGCAATCAGC
      3901 TGTTGCCCGT CTCACTGGTG AAAAGAAAAA CCACCCTGGC GCCCAATACG CAAACCGCCT
      3961 CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA
      4021 GCGGGCAGTG AGCGCAACGC AATTAATGTA AGTTAGCTCA CTCATTAGGC ACCGGGATCT
      4081 CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG
      4141 ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG
      4201 GCAGCGCTCT GGGTCATTTT CGGCGAGGAC CGCTTTCGCT GGAGCGCGAC GATGATCGGC
      4261 CTGTCGCTTG CGGTATTCGG AATCTTGCAC GCCCTCGCTC AAGCCTTCGT CACTGGTCCC
      4321 GCCACCAAAC GTTTCGGCGA GAAGCAGGCC ATTATCGCCG GCATGGCGGC CCCACGGGTG
      4381 CGCATGATCG TGCTCCTGTC GTTGAGGACC CGGCTAGGCT GGCGGGGTTG CCTTACTGGT
      4441 TAGCAGAATG AATCACCGAT ACGCGAGCGA ACGTGAAGCG ACTGCTGCTG CAAAACGTCT
      4501 GCGACCTGAG CAACAACATG AATGGTCTTC GGTTTCCGTG TTTCGTAAAG TCTGGAAACG
      4561 CGGAAGTCAG CGCCCTGCAC CATTATGTTC CGGATCTGCA TCGCAGGATG CTGCTGGCTA
      4621 CCCTGTGGAA CACCTACATC TGTATTAACG AAGCGCTGGC ATTGACCCTG AGTGATTTTT
      4681 CTCTGGTCCC GCCGCATCCA TACCGCCAGT TGTTTACCCT CACAACGTTC CAGTAACCGG
      4741 GCATGTTCAT CATCAGTAAC CCGTATCGTG AGCATCCTCT CTCGTTTCAT CGGTATCATT
      4801 ACCCCCATGA ACAGAAATCC CCCTTACACG GAGGCATCAG TGACCAAACA GGAAAAAACC
      4861 GCCCTTAACA TGGCCCGCTT TATCAGAAGC CAGACATTAA CGCTTCTGGA GAAACTCAAC
      4921 GAGCTGGACG CGGATGAACA GGCAGACATC TGTGAATCGC TTCACGACCA CGCTGATGAG
      4981 CTTTACCGCA GCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG
      5041 CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG
      5101 GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCGCAGCCA TGACCCAGTC ACGTAGCGAT
      5161 AGCGGAGTGT ATACTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC
      5221 ATATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCTC
      5281 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC
      5341 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA
      5401 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
      5461 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
      5521 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
      5581 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
      5641 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
      5701 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
      5761 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
      5821 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
      5881 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
      5941 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
      6001 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
      6061 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
      6121 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
      6181 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
      6241 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
      6301 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
      6361 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
      6421 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
      6481 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTGCAGG
      6541 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
      6601 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
      6661 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
      6721 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
      6781 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
      6841 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
      6901 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
      6961 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
      7021 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
      7081 ACTCTTCCTT TTTCAAATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
      7141 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
      7201 GTGCCACCTA AATTGTAAGC GTTAATGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC
      7261 GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG
      7321 GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG
      7381 GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC
      7441 GCCGCTACAG GGCGCGTCCC ATTCGCCA
//