FEATURES             Location/Qualifiers
     promoter        214..230
                     /label="T7\promoter
     CDS             complement(2630..3589)
                     /label="lacI
     protein_bind    5..25
                     /label="lacO
     RBS             58..63
                     /label="RBS-1
     misc_binding    83..100
                     /label="His-Tag
     protein_bind    235..255
                     /label="lacO
     RBS             286..291
                     /label="RBS
     rep_origin      1773..1773
                     /label="Replication\Origin
     -35_signal      complement(1607..1612)
                     /label="-35\Ap
     -10_signal      complement(1584..1590)
                     /label="-10\Ap
     RBS             complement(1548..1552)
                     /label="RBS
     CDS             complement(751..1542)
                     /label="aadA\(Correct,\original\source\Tn21)
     rep_origin      complement(1682..2420)
                     /label="CloDF13\ori
     CDS             71..160
                     /note="mcs-1 orf"
     misc_feature    353..384
                     /note="LIC Site"
     Cleavage-site   345..365
                     /note="TEV site"
     Region          303..320
                     /note="His Tag"
     CDS             300..383
                     /note="coding region"

         1 GGGGAATTGT GAGCGGATAA CAATTCCCCT GTAGAAATAA TTTTGTTTAA CTTTAATAAG
        61 GAGATATACC ATGGGCAGCA GCCATCACCA TCATCACCAC AGCCAGGATC CGAATTCGAG
       121 CTCGGCGCGC CTGCAGGTCG ACAAGCTTGC GGCCGCATAA TGCTTAAGTC GAACAGAAAG
       181 TAATCGTATT GTACACGGCC GCATAATCGA AATTAATACG ACTCACTATA GGGGAATTGT
       241 GAGCGGATAA CAATTCCCCA TCTTAGTATA TTAGTTAAGT ATAAGAAGGA GATATACATA
       301 TGCACCATCA TCATCATCAT TCTTCTGGTG TAGATCTGGG TACCGAGAAC CTGTACTTCC
       361 AATCCAATAT TGGAAGTGGA TAACGGATCC GAATTCGAGC TCCGTCGACA AGCTTGCGGC
       421 CGCACTCGAG TCTGGTAAAG AAACCGCTGC TGCGAAATTT GAACGCCAGC ACATGGACTC
       481 GTCTACTAGC GCAGCTTAAT TAACCTAGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA
       541 ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAACCTC AGGCATTTGA
       601 GAAGCACACG GTCACACTGC TTCCGGTAGT CAATAAACCG GTAAACCAGC AATAGACATA
       661 AGCGGCTATT TAACGACCCT GCCCTGAACC GACGACCGGG TCATCGTGGC CGGATCTTGC
       721 GGCCCCTCGG CTTGAACGAA TTGTTAGACA TTATTTGCCG ACTACCTTGG TGATCTCGCC
       781 TTTCACGTAG TGGACAAATT CTTCCAACTG ATCTGCGCGC GAGGCCAAGC GATCTTCTTC
       841 TTGTCCAAGA TAAGCCTGTC TAGCTTCAAG TATGACGGGC TGATACTGGG CCGGCAGGCG
       901 CTCCATTGCC CAGTCGGCAG CGACATCCTT CGGCGCGATT TTGCCGGTTA CTGCGCTGTA
       961 CCAAATGCGG GACAACGTAA GCACTACATT TCGCTCATCG CCAGCCCAGT CGGGCGGCGA
      1021 GTTCCATAGC GTTAAGGTTT CATTTAGCGC CTCAAATAGA TCCTGTTCAG GAACCGGATC
      1081 AAAGAGTTCC TCCGCCGCTG GACCTACCAA GGCAACGCTA TGTTCTCTTG CTTTTGTCAG
      1141 CAAGATAGCC AGATCAATGT CGATCGTGGC TGGCTCGAAG ATACCTGCAA GAATGTCATT
      1201 GCGCTGCCAT TCTCCAAATT GCAGTTCGCG CTTAGCTGGA TAACGCCACG GAATGATGTC
      1261 GTCGTGCACA ACAATGGTGA CTTCTACAGC GCGGAGAATC TCGCTCTCTC CAGGGGAAGC
      1321 CGAAGTTTCC AAAAGGTCGT TGATCAAAGC TCGCCGCGTT GTTTCATCAA GCCTTACGGT
      1381 CACCGTAACC AGCAAATCAA TATCACTGTG TGGCTTCAGG CCGCCATCCA CTGCGGAGCC
      1441 GTACAAATGT ACGGCCAGCA ACGTCGGTTC GAGATGGCGC TCGATGACGC CAACTACCTC
      1501 TGATAGTTGA GTCGATACTT CGGCGATCAC CGCTTCCCTC ATACTCTTCC TTTTTCATTA
      1561 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
      1621 GAAAAATAAA CAAATAGCTA GCTCACTCGG TCGCTACGCT CCGGGCGTGA GACTGCGGCG
      1681 GGCGCTGCGG ACACATACAA AGTTACCCAC AGATTCCGTG GATAAGCAGG GGACTAACAT
      1741 GTGAGGCAAA ACAGCAGGGC CGCGCCGGTG GCGTTTTTCC ATAGGCTCCG CCCTCCTGCC
      1801 AGAGTTCACA TAAACAGACG CTTTTCCGGT GCATCTGTGG GAGCCGTGAG GCTCAACCAT
      1861 GAATCTGACA GTACGGGCGA AACCCGACAG GACTTAAAGA TCCCCACCGT TTCCGGCGGG
      1921 TCGCTCCCTC TTGCGCTCTC CTGTTCCGAC CCTGCCGTTT ACCGGATACC TGTTCCGCCT
      1981 TTCTCCCTTA CGGGAAGTGT GGCGCTTTCT CATAGCTCAC ACACTGGTAT CTCGGCTCGG
      2041 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTA AGCAAGAACT CCCCGTTCAG CCCGACTGCT
      2101 GCGCCTTATC CGGTAACTGT TCACTTGAGT CCAACCCGGA AAAGCACGGT AAAACGCCAC
      2161 TGGCAGCAGC CATTGGTAAC TGGGAGTTCG CAGAGGATTT GTTTAGCTAA ACACGCGGTT
      2221 GCTCTTGAAG TGTGCGCCAA AGTCCGGCTA CACTGGAAGG ACAGATTTGG TTGCTGTGCT
      2281 CTGCGAAAGC CAGTTACCAC GGTTAAGCAG TTCCCCAACT GACTTAACCT TCGATCAAAC
      2341 CACCTCCCCA GGTGGTTTTT TCGTTTACAG GGCAAAAGAT TACGCGCAGA AAAAAAGGAT
      2401 CTCAAGAAGA TCCTTTGATC TTTTCTACTG AACCGCTCTA GATTTCAGTG CAATTTATCT
      2461 CTTCAAATGT AGCACCTGAA GTCAGCCCCA TACGATATAA GTTGTAATTC TCATGTTAGT
      2521 CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG
      2581 AGATCCCGGT GCCTAATGAG TGAGCTAACT TACATTAATT GCGTTGCGCT CACTGCCCGC
      2641 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG
      2701 AGGCGGTTTG CGTATTGGGC GCCAGGGTGG TTTTTCTTTT CACCAGTGAG ACGGGCAACA
      2761 GCTGATTGCC CTTCACCGCC TGGCCCTGAG AGAGTTGCAG CAAGCGGTCC ACGCTGGTTT
      2821 GCCCCAGCAG GCGAAAATCC TGTTTGATGG TGGTTAACGG CGGGATATAA CATGAGCTGT
      2881 CTTCGGTATC GTCGTATCCC ACTACCGAGA TGTCCGCACC AACGCGCAGC CCGGACTCGG
      2941 TAATGGCGCG CATTGCGCCC AGCGCCATCT GATCGTTGGC AACCAGCATC GCAGTGGGAA
      3001 CGATGCCCTC ATTCAGCATT TGCATGGTTT GTTGAAAACC GGACATGGCA CTCCAGTCGC
      3061 CTTCCCGTTC CGCTATCGGC TGAATTTGAT TGCGAGTGAG ATATTTATGC CAGCCAGCCA
      3121 GACGCAGACG CGCCGAGACA GAACTTAATG GGCCCGCTAA CAGCGCGATT TGCTGGTGAC
      3181 CCAATGCGAC CAGATGCTCC ACGCCCAGTC GCGTACCGTC TTCATGGGAG AAAATAATAC
      3241 TGTTGATGGG TGTCTGGTCA GAGACATCAA GAAATAACGC CGGAACATTA GTGCAGGCAG
      3301 CTTCCACAGC AATGGCATCC TGGTCATCCA GCGGATAGTT AATGATCAGC CCACTGACGC
      3361 GTTGCGCGAG AAGATTGTGC ACCGCCGCTT TACAGGCTTC GACGCCGCTT CGTTCTACCA
      3421 TCGACACCAC CACGCTGGCA CCCAGTTGAT CGGCGCGAGA TTTAATCGCC GCGACAATTT
      3481 GCGACGGCGC GTGCAGGGCC AGACTGGAGG TGGCAACGCC AATCAGCAAC GACTGTTTGC
      3541 CCGCCAGTTG TTGTGCCACG CGGTTGGGAA TGTAATTCAG CTCCGCCATC GCCGCTTCCA
      3601 CTTTTTCCCG CGTTTTCGCA GAAACGTGGC TGGCCTGGTT CACCACGCGG GAAACGGTCT
      3661 GATAAGAGAC ACCGGCATAC TCTGCGACAT CGTATAACGT TACTGGTTTC ACATTCACCA
      3721 CCCTGAATTG ACTCTCTTCC GGGCGCTATC ATGCCATACC GCGAAAGGTT TTGCGCCATT
      3781 CGATGGTGTC CGGGATCTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAATTAATA
      3841 CGACTCACTA TA
//