FEATURES Location/Qualifiers CDS complement(2684..3643) /label="lacI promoter 3890..3906 /label="T7\Promoter protein_bind 5..25 /label="lacO RBS 58..63 /label="RBS-1 Region 83..100 /label="His-Tag terminator 587..634 /label="T7\Terminator protein_bind 235..255 /label="lacO promoter 214..230 /label="T7\promoter RBS 286..291 /label="RBS rep_origin 1827..1827 /label="Replication\Origin -35_signal complement(1661..1666) /label="-35\Ap -10_signal complement(1638..1644) /label="-10\Ap RBS complement(1602..1606) /label="RBS CDS complement(805..1596) /label="aadA\(Correct,\original\source\Tn21) rep_origin complement(1736..2474) /label="CloDF13\ori CDS 71..160 /note="mcs-1 orf" misc_feature 407..438 /note="LIC Site" Cleavage-site 399..419 /note="TEV site" Region 303..320 /note="His Tag" Region 339..392 /note="S loop" CDS 300..437 /note="coding region" 1 GGGGAATTGT GAGCGGATAA CAATTCCCCT GTAGAAATAA TTTTGTTTAA CTTTAATAAG 61 GAGATATACC ATGGGCAGCA GCCATCACCA TCATCACCAC AGCCAGGATC CGAATTCGAG 121 CTCGGCGCGC CTGCAGGTCG ACAAGCTTGC GGCCGCATAA TGCTTAAGTC GAACAGAAAG 181 TAATCGTATT GTACACGGCC GCATAATCGA AATTAATACG ACTCACTATA GGGGAATTGT 241 GAGCGGATAA CAATTCCCCA TCTTAGTATA TTAGTTAAGT ATAAGAAGGA GATATACATA 301 TGCACCATCA TCATCATCAT TCTTCTGGTG TAGATCTGGA AGTTGAAACC AAATCTGCTG 361 GTGGTATCGT TCTGACCGGT TCTGCTGCTG CTGGTACCGA GAACCTGTAC TTCCAATCCA 421 ATATTGGAAG TGGATAACGG ATCCGAATTC GAGCTCCGTC GACAAGCTTG CGGCCGCACT 481 CGAGTCTGGT AAAGAAACCG CTGCTGCGAA ATTTGAACGC CAGCACATGG ACTCGTCTAC 541 TAGCGCAGCT TAATTAACCT AGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT 601 TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA CCTCAGGCAT TTGAGAAGCA 661 CACGGTCACA CTGCTTCCGG TAGTCAATAA ACCGGTAAAC CAGCAATAGA CATAAGCGGC 721 TATTTAACGA CCCTGCCCTG AACCGACGAC CGGGTCATCG TGGCCGGATC TTGCGGCCCC 781 TCGGCTTGAA CGAATTGTTA GACATTATTT GCCGACTACC TTGGTGATCT CGCCTTTCAC 841 GTAGTGGACA AATTCTTCCA ACTGATCTGC GCGCGAGGCC AAGCGATCTT CTTCTTGTCC 901 AAGATAAGCC TGTCTAGCTT CAAGTATGAC GGGCTGATAC TGGGCCGGCA GGCGCTCCAT 961 TGCCCAGTCG GCAGCGACAT CCTTCGGCGC GATTTTGCCG GTTACTGCGC TGTACCAAAT 1021 GCGGGACAAC GTAAGCACTA CATTTCGCTC ATCGCCAGCC CAGTCGGGCG GCGAGTTCCA 1081 TAGCGTTAAG GTTTCATTTA GCGCCTCAAA TAGATCCTGT TCAGGAACCG GATCAAAGAG 1141 TTCCTCCGCC GCTGGACCTA CCAAGGCAAC GCTATGTTCT CTTGCTTTTG TCAGCAAGAT 1201 AGCCAGATCA ATGTCGATCG TGGCTGGCTC GAAGATACCT GCAAGAATGT CATTGCGCTG 1261 CCATTCTCCA AATTGCAGTT CGCGCTTAGC TGGATAACGC CACGGAATGA TGTCGTCGTG 1321 CACAACAATG GTGACTTCTA CAGCGCGGAG AATCTCGCTC TCTCCAGGGG AAGCCGAAGT 1381 TTCCAAAAGG TCGTTGATCA AAGCTCGCCG CGTTGTTTCA TCAAGCCTTA CGGTCACCGT 1441 AACCAGCAAA TCAATATCAC TGTGTGGCTT CAGGCCGCCA TCCACTGCGG AGCCGTACAA 1501 ATGTACGGCC AGCAACGTCG GTTCGAGATG GCGCTCGATG ACGCCAACTA CCTCTGATAG 1561 TTGAGTCGAT ACTTCGGCGA TCACCGCTTC CCTCATACTC TTCCTTTTTC ATTATTATTG 1621 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 1681 TAAACAAATA GCTAGCTCAC TCGGTCGCTA CGCTCCGGGC GTGAGACTGC GGCGGGCGCT 1741 GCGGACACAT ACAAAGTTAC CCACAGATTC CGTGGATAAG CAGGGGACTA ACATGTGAGG 1801 CAAAACAGCA GGGCCGCGCC GGTGGCGTTT TTCCATAGGC TCCGCCCTCC TGCCAGAGTT 1861 CACATAAACA GACGCTTTTC CGGTGCATCT GTGGGAGCCG TGAGGCTCAA CCATGAATCT 1921 GACAGTACGG GCGAAACCCG ACAGGACTTA AAGATCCCCA CCGTTTCCGG CGGGTCGCTC 1981 CCTCTTGCGC TCTCCTGTTC CGACCCTGCC GTTTACCGGA TACCTGTTCC GCCTTTCTCC 2041 CTTACGGGAA GTGTGGCGCT TTCTCATAGC TCACACACTG GTATCTCGGC TCGGTGTAGG 2101 TCGTTCGCTC CAAGCTGGGC TGTAAGCAAG AACTCCCCGT TCAGCCCGAC TGCTGCGCCT 2161 TATCCGGTAA CTGTTCACTT GAGTCCAACC CGGAAAAGCA CGGTAAAACG CCACTGGCAG 2221 CAGCCATTGG TAACTGGGAG TTCGCAGAGG ATTTGTTTAG CTAAACACGC GGTTGCTCTT 2281 GAAGTGTGCG CCAAAGTCCG GCTACACTGG AAGGACAGAT TTGGTTGCTG TGCTCTGCGA 2341 AAGCCAGTTA CCACGGTTAA GCAGTTCCCC AACTGACTTA ACCTTCGATC AAACCACCTC 2401 CCCAGGTGGT TTTTTCGTTT ACAGGGCAAA AGATTACGCG CAGAAAAAAA GGATCTCAAG 2461 AAGATCCTTT GATCTTTTCT ACTGAACCGC TCTAGATTTC AGTGCAATTT ATCTCTTCAA 2521 ATGTAGCACC TGAAGTCAGC CCCATACGAT ATAAGTTGTA ATTCTCATGT TAGTCATGCC 2581 CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGAGATCC 2641 CGGTGCCTAA TGAGTGAGCT AACTTACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA 2701 GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG 2761 TTTGCGTATT GGGCGCCAGG GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT 2821 TGCCCTTCAC CGCCTGGCCC TGAGAGAGTT GCAGCAAGCG GTCCACGCTG GTTTGCCCCA 2881 GCAGGCGAAA ATCCTGTTTG ATGGTGGTTA ACGGCGGGAT ATAACATGAG CTGTCTTCGG 2941 TATCGTCGTA TCCCACTACC GAGATGTCCG CACCAACGCG CAGCCCGGAC TCGGTAATGG 3001 CGCGCATTGC GCCCAGCGCC ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC 3061 CCTCATTCAG CATTTGCATG GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC 3121 GTTCCGCTAT CGGCTGAATT TGATTGCGAG TGAGATATTT ATGCCAGCCA GCCAGACGCA 3181 GACGCGCCGA GACAGAACTT AATGGGCCCG CTAACAGCGC GATTTGCTGG TGACCCAATG 3241 CGACCAGATG CTCCACGCCC AGTCGCGTAC CGTCTTCATG GGAGAAAATA ATACTGTTGA 3301 TGGGTGTCTG GTCAGAGACA TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA 3361 CAGCAATGGC ATCCTGGTCA TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACGCGTTGCG 3421 CGAGAAGATT GTGCACCGCC GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA 3481 CCACCACGCT GGCACCCAGT TGATCGGCGC GAGATTTAAT CGCCGCGACA ATTTGCGACG 3541 GCGCGTGCAG GGCCAGACTG GAGGTGGCAA CGCCAATCAG CAACGACTGT TTGCCCGCCA 3601 GTTGTTGTGC CACGCGGTTG GGAATGTAAT TCAGCTCCGC CATCGCCGCT TCCACTTTTT 3661 CCCGCGTTTT CGCAGAAACG TGGCTGGCCT GGTTCACCAC GCGGGAAACG GTCTGATAAG 3721 AGACACCGGC ATACTCTGCG ACATCGTATA ACGTTACTGG TTTCACATTC ACCACCCTGA 3781 ATTGACTCTC TTCCGGGCGC TATCATGCCA TACCGCGAAA GGTTTTGCGC CATTCGATGG 3841 TGTCCGGGAT CTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAATT AATACGACTC 3901 ACTATA //