FEATURES             Location/Qualifiers
     CDS             complement(3749..4708)
                     /label="lacI
     promoter        4955..4971
                     /label="T7\Promoter
     protein_bind    5..25
                     /label="lacO
     RBS             58..63
                     /label="RBS-1
     Region          83..100
                     /label="His-Tag
     terminator      1652..1699
                     /label="T7\Terminator
     protein_bind    235..255
                     /label="lacO
     promoter        214..230
                     /label="T7\promoter
     RBS             286..291
                     /label="RBS
     rep_origin      2892..2892
                     /label="Replication\Origin
     -35_signal      complement(2726..2731)
                     /label="-35\Ap
     -10_signal      complement(2703..2709)
                     /label="-10\Ap
     RBS             complement(2667..2671)
                     /label="RBS
     CDS             complement(1870..2661)
                     /label="aadA\(Correct,\original\source\Tn21)
     rep_origin      complement(2801..3539)
                     /label="CloDF13\ori
     CDS             71..160
                     /note="mcs-1 orf"
     misc_feature    1472..1503
                     /note="LIC Site"
     Cleavage-site   1464..1484
                     /note="TEV site"
     Region          303..320
                     /note="His Tag"
     CDS             300..1502
                     /note="coding region"
     Region          345..1442
                     /note="MBP;MBP"

         1 GGGGAATTGT GAGCGGATAA CAATTCCCCT GTAGAAATAA TTTTGTTTAA CTTTAATAAG
        61 GAGATATACC ATGGGCAGCA GCCATCACCA TCATCACCAC AGCCAGGATC CGAATTCGAG
       121 CTCGGCGCGC CTGCAGGTCG ACAAGCTTGC GGCCGCATAA TGCTTAAGTC GAACAGAAAG
       181 TAATCGTATT GTACACGGCC GCATAATCGA AATTAATACG ACTCACTATA GGGGAATTGT
       241 GAGCGGATAA CAATTCCCCA TCTTAGTATA TTAGTTAAGT ATAAGAAGGA GATATACATA
       301 TGCACCATCA TCATCATCAT TCTTCTGGTG TAGATCTGGG TACCAAAATC GAAGAAGGTA
       361 AACTGGTAAT CTGGATTAAC GGCGATAAAG GCTATAACGG TCTCGCTGAA GTCGGTAAGA
       421 AATTCGAGAA AGATACCGGA ATTAAAGTCA CCGTTGAGCA TCCGGATAAA CTGGAAGAGA
       481 AATTCCCACA GGTTGCGGCA ACTGGCGATG GCCCTGACAT TATCTTCTGG GCACACGACC
       541 GCTTTGGTGG CTACGCTCAA TCTGGCCTGT TGGCTGAAAT CACCCCGGAC AAAGCGTTCC
       601 AGGACAAGCT GTATCCGTTT ACCTGGGATG CCGTACGTTA CAACGGCAAG CTGATTGCTT
       661 ACCCGATCGC TGTTGAAGCG TTATCGCTGA TTTATAACAA AGATCTGCTG CCGAACCCGC
       721 CAAAAACCTG GGAAGAGATC CCGGCGCTGG ATAAAGAACT GAAAGCGAAA GGTAAGAGCG
       781 CGCTGATGTT CAACCTGCAA GAACCGTACT TCACCTGGCC GCTGATTGCT GCTGACGGGG
       841 GTTATGCGTT CAAGTATGAA AACGGCAAGT ACGACATTAA AGACGTGGGC GTGGATAACG
       901 CTGGCGCGAA AGCGGGTCTG ACCTTCCTGG TTGACCTGAT TAAAAACAAA CACATGAATG
       961 CAGACACCGA TTACTCCATC GCAGAAGCTG CCTTTAATAA AGGCGAAACA GCGATGACCA
      1021 TCAACGGCCC GTGGGCATGG TCCAACATCG ACACCAGCAA AGTGAATTAT GGTGTAACGG
      1081 TACTGCCGAC CTTCAAGGGT CAACCATCCA AACCGTTCGT TGGCGTGCTG AGCGCAGGTA
      1141 TTAACGCCGC CAGTCCGAAC AAAGAGCTGG CAAAAGAGTT CCTCGAAAAC TATCTGCTGA
      1201 CTGATGAAGG TCTGGAAGCG GTTAATAAAG ACAAACCGCT GGGTGCCGTA GCGCTGAAGT
      1261 CTTACGAGGA AGAGTTGGCG AAAGATCCAC GTATTGCCGC CACCATGGAA AACGCCCAGA
      1321 AAGGTGAAAT CATGCCGAAC ATCCCGCAGA TGTCCGCTTT CTGGTATGCC GTGCGTACTG
      1381 CGGTGATCAA CGCCGCCAGC GGTCGTCAGA CTGTCGATGA AGCCCTGAAA GACGCGCAGA
      1441 CTGATTACGA TATCCCAGGT ACCGAGAACC TGTACTTCCA ATCCAATATT GGAAGTGGAT
      1501 AACGGATCCG AATTCGAGCT CCGTCGACAA GCTTGCGGCC GCACTCGAGT CTGGTAAAGA
      1561 AACCGCTGCT GCGAAATTTG AACGCCAGCA CATGGACTCG TCTACTAGCG CAGCTTAATT
      1621 AACCTAGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG
      1681 GGTCTTGAGG GGTTTTTTGC TGAAACCTCA GGCATTTGAG AAGCACACGG TCACACTGCT
      1741 TCCGGTAGTC AATAAACCGG TAAACCAGCA ATAGACATAA GCGGCTATTT AACGACCCTG
      1801 CCCTGAACCG ACGACCGGGT CATCGTGGCC GGATCTTGCG GCCCCTCGGC TTGAACGAAT
      1861 TGTTAGACAT TATTTGCCGA CTACCTTGGT GATCTCGCCT TTCACGTAGT GGACAAATTC
      1921 TTCCAACTGA TCTGCGCGCG AGGCCAAGCG ATCTTCTTCT TGTCCAAGAT AAGCCTGTCT
      1981 AGCTTCAAGT ATGACGGGCT GATACTGGGC CGGCAGGCGC TCCATTGCCC AGTCGGCAGC
      2041 GACATCCTTC GGCGCGATTT TGCCGGTTAC TGCGCTGTAC CAAATGCGGG ACAACGTAAG
      2101 CACTACATTT CGCTCATCGC CAGCCCAGTC GGGCGGCGAG TTCCATAGCG TTAAGGTTTC
      2161 ATTTAGCGCC TCAAATAGAT CCTGTTCAGG AACCGGATCA AAGAGTTCCT CCGCCGCTGG
      2221 ACCTACCAAG GCAACGCTAT GTTCTCTTGC TTTTGTCAGC AAGATAGCCA GATCAATGTC
      2281 GATCGTGGCT GGCTCGAAGA TACCTGCAAG AATGTCATTG CGCTGCCATT CTCCAAATTG
      2341 CAGTTCGCGC TTAGCTGGAT AACGCCACGG AATGATGTCG TCGTGCACAA CAATGGTGAC
      2401 TTCTACAGCG CGGAGAATCT CGCTCTCTCC AGGGGAAGCC GAAGTTTCCA AAAGGTCGTT
      2461 GATCAAAGCT CGCCGCGTTG TTTCATCAAG CCTTACGGTC ACCGTAACCA GCAAATCAAT
      2521 ATCACTGTGT GGCTTCAGGC CGCCATCCAC TGCGGAGCCG TACAAATGTA CGGCCAGCAA
      2581 CGTCGGTTCG AGATGGCGCT CGATGACGCC AACTACCTCT GATAGTTGAG TCGATACTTC
      2641 GGCGATCACC GCTTCCCTCA TACTCTTCCT TTTTCATTAT TATTGAAGCA TTTATCAGGG
      2701 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGCTAG
      2761 CTCACTCGGT CGCTACGCTC CGGGCGTGAG ACTGCGGCGG GCGCTGCGGA CACATACAAA
      2821 GTTACCCACA GATTCCGTGG ATAAGCAGGG GACTAACATG TGAGGCAAAA CAGCAGGGCC
      2881 GCGCCGGTGG CGTTTTTCCA TAGGCTCCGC CCTCCTGCCA GAGTTCACAT AAACAGACGC
      2941 TTTTCCGGTG CATCTGTGGG AGCCGTGAGG CTCAACCATG AATCTGACAG TACGGGCGAA
      3001 ACCCGACAGG ACTTAAAGAT CCCCACCGTT TCCGGCGGGT CGCTCCCTCT TGCGCTCTCC
      3061 TGTTCCGACC CTGCCGTTTA CCGGATACCT GTTCCGCCTT TCTCCCTTAC GGGAAGTGTG
      3121 GCGCTTTCTC ATAGCTCACA CACTGGTATC TCGGCTCGGT GTAGGTCGTT CGCTCCAAGC
      3181 TGGGCTGTAA GCAAGAACTC CCCGTTCAGC CCGACTGCTG CGCCTTATCC GGTAACTGTT
      3241 CACTTGAGTC CAACCCGGAA AAGCACGGTA AAACGCCACT GGCAGCAGCC ATTGGTAACT
      3301 GGGAGTTCGC AGAGGATTTG TTTAGCTAAA CACGCGGTTG CTCTTGAAGT GTGCGCCAAA
      3361 GTCCGGCTAC ACTGGAAGGA CAGATTTGGT TGCTGTGCTC TGCGAAAGCC AGTTACCACG
      3421 GTTAAGCAGT TCCCCAACTG ACTTAACCTT CGATCAAACC ACCTCCCCAG GTGGTTTTTT
      3481 CGTTTACAGG GCAAAAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
      3541 TTTCTACTGA ACCGCTCTAG ATTTCAGTGC AATTTATCTC TTCAAATGTA GCACCTGAAG
      3601 TCAGCCCCAT ACGATATAAG TTGTAATTCT CATGTTAGTC ATGCCCCGCG CCCACCGGAA
      3661 GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA GATCCCGGTG CCTAATGAGT
      3721 GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC
      3781 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
      3841 CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT
      3901 GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT
      3961 GTTTGATGGT GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA
      4021 CTACCGAGAT GTCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA
      4081 GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT
      4141 GCATGGTTTG TTGAAAACCG GACATGGCAC TCCAGTCGCC TTCCCGTTCC GCTATCGGCT
      4201 GAATTTGATT GCGAGTGAGA TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG
      4261 AACTTAATGG GCCCGCTAAC AGCGCGATTT GCTGGTGACC CAATGCGACC AGATGCTCCA
      4321 CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG
      4381 AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGCATCCT
      4441 GGTCATCCAG CGGATAGTTA ATGATCAGCC CACTGACGCG TTGCGCGAGA AGATTGTGCA
      4501 CCGCCGCTTT ACAGGCTTCG ACGCCGCTTC GTTCTACCAT CGACACCACC ACGCTGGCAC
      4561 CCAGTTGATC GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGGCCA
      4621 GACTGGAGGT GGCAACGCCA ATCAGCAACG ACTGTTTGCC CGCCAGTTGT TGTGCCACGC
      4681 GGTTGGGAAT GTAATTCAGC TCCGCCATCG CCGCTTCCAC TTTTTCCCGC GTTTTCGCAG
      4741 AAACGTGGCT GGCCTGGTTC ACCACGCGGG AAACGGTCTG ATAAGAGACA CCGGCATACT
      4801 CTGCGACATC GTATAACGTT ACTGGTTTCA CATTCACCAC CCTGAATTGA CTCTCTTCCG
      4861 GGCGCTATCA TGCCATACCG CGAAAGGTTT TGCGCCATTC GATGGTGTCC GGGATCTCGA
      4921 CGCTCTCCCT TATGCGACTC CTGCATTAGG AAATTAATAC GACTCACTAT A
//