FEATURES             Location/Qualifiers
     CDS             complement(3305..4264)
                     /label="lacI
     promoter        4511..4527
                     /label="T7\Promoter
     protein_bind    5..25
                     /label="lacO
     RBS             58..63
                     /label="RBS-1
     Region          83..100
                     /label="His-Tag
     terminator      1208..1255
                     /label="T7\Terminator
     protein_bind    235..255
                     /label="lacO
     promoter        214..230
                     /label="T7\promoter
     RBS             286..291
                     /label="RBS
     rep_origin      2448..2448
                     /label="Replication\Origin
     -35_signal      complement(2282..2287)
                     /label="-35\Ap
     -10_signal      complement(2259..2265)
                     /label="-10\Ap
     RBS             complement(2223..2227)
                     /label="RBS
     CDS             complement(1426..2217)
                     /label="aadA\(Correct,\original\source\Tn21)
     rep_origin      complement(2357..3095)
                     /label="CloDF13\ori
     CDS             71..160
                     /note="mcs-1 orf"
     misc_feature    1028..1059
                     /note="LIC Site"
     Cleavage-site   1020..1040
                     /note="TEV site"
     Region          303..320
                     /note="His Tag"
     CDS             300..1058
                     /note="coding region"
     Region          339..998
                     /note="GST;GST"

         1 GGGGAATTGT GAGCGGATAA CAATTCCCCT GTAGAAATAA TTTTGTTTAA CTTTAATAAG
        61 GAGATATACC ATGGGCAGCA GCCATCACCA TCATCACCAC AGCCAGGATC CGAATTCGAG
       121 CTCGGCGCGC CTGCAGGTCG ACAAGCTTGC GGCCGCATAA TGCTTAAGTC GAACAGAAAG
       181 TAATCGTATT GTACACGGCC GCATAATCGA AATTAATACG ACTCACTATA GGGGAATTGT
       241 GAGCGGATAA CAATTCCCCA TCTTAGTATA TTAGTTAAGT ATAAGAAGGA GATATACATA
       301 TGCACCATCA TCATCATCAT TCTTCTGGTG TAGATCTGAT GTCCCCTATA CTAGGTTATT
       361 GGAAAATTAA GGGCCTTGTG CAACCCACTC GACTTCTTTT GGAATATCTT GAAGAAAAAT
       421 ATGAAGAGCA TTTGTATGAG CGCGATGAAG GTGATAAATG GCGAAACAAA AAGTTTGAAT
       481 TGGGTTTGGA GTTTCCCAAT CTTCCTTATT ATATTGATGG TGATGTTAAA TTAACACAGT
       541 CTATGGCCAT CATACGTTAT ATAGCTGACA AGCACAACAT GTTGGGTGGT TGTCCAAAAG
       601 AGCGTGCAGA GATTTCAATG CTTGAAGGAG CGGTTTTGGA TATTAGATAC GGTGTTTCGA
       661 GAATTGCATA TAGTAAAGAC TTTGAAACTC TCAAAGTTGA TTTTCTTAGC AAGCTACCTG
       721 AAATGCTGAA AATGTTCGAA GATCGTTTAT GTCATAAAAC ATATTTAAAT GGTGATCATG
       781 TAACCCATCC TGACTTCATG TTGTATGACG CTCTTGATGT TGTTTTATAC ATGGACCCAA
       841 TGTGCCTGGA TGCGTTCCCA AAATTAGTTT GTTTTAAAAA ACGTATTGAA GCTATCCCAC
       901 AAATTGATAA GTACTTGAAA TCCAGCAAGT ATATAGCATG GCCTTTGCAG GGCTGGCAAG
       961 CCACGTTTGG TGGTGGCGAC CATCCTCCAA AATCGGATGA TTACCCAGAT CTGGGTACCG
      1021 AGAACCTGTA CTTCCAATCC AATATTGGAA GTGGATAACG GATCCGAATT CGAGCTCCGT
      1081 CGACAAGCTT GCGGCCGCAC TCGAGTCTGG TAAAGAAACC GCTGCTGCGA AATTTGAACG
      1141 CCAGCACATG GACTCGTCTA CTAGCGCAGC TTAATTAACC TAGGCTGCTG CCACCGCTGA
      1201 GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA
      1261 ACCTCAGGCA TTTGAGAAGC ACACGGTCAC ACTGCTTCCG GTAGTCAATA AACCGGTAAA
      1321 CCAGCAATAG ACATAAGCGG CTATTTAACG ACCCTGCCCT GAACCGACGA CCGGGTCATC
      1381 GTGGCCGGAT CTTGCGGCCC CTCGGCTTGA ACGAATTGTT AGACATTATT TGCCGACTAC
      1441 CTTGGTGATC TCGCCTTTCA CGTAGTGGAC AAATTCTTCC AACTGATCTG CGCGCGAGGC
      1501 CAAGCGATCT TCTTCTTGTC CAAGATAAGC CTGTCTAGCT TCAAGTATGA CGGGCTGATA
      1561 CTGGGCCGGC AGGCGCTCCA TTGCCCAGTC GGCAGCGACA TCCTTCGGCG CGATTTTGCC
      1621 GGTTACTGCG CTGTACCAAA TGCGGGACAA CGTAAGCACT ACATTTCGCT CATCGCCAGC
      1681 CCAGTCGGGC GGCGAGTTCC ATAGCGTTAA GGTTTCATTT AGCGCCTCAA ATAGATCCTG
      1741 TTCAGGAACC GGATCAAAGA GTTCCTCCGC CGCTGGACCT ACCAAGGCAA CGCTATGTTC
      1801 TCTTGCTTTT GTCAGCAAGA TAGCCAGATC AATGTCGATC GTGGCTGGCT CGAAGATACC
      1861 TGCAAGAATG TCATTGCGCT GCCATTCTCC AAATTGCAGT TCGCGCTTAG CTGGATAACG
      1921 CCACGGAATG ATGTCGTCGT GCACAACAAT GGTGACTTCT ACAGCGCGGA GAATCTCGCT
      1981 CTCTCCAGGG GAAGCCGAAG TTTCCAAAAG GTCGTTGATC AAAGCTCGCC GCGTTGTTTC
      2041 ATCAAGCCTT ACGGTCACCG TAACCAGCAA ATCAATATCA CTGTGTGGCT TCAGGCCGCC
      2101 ATCCACTGCG GAGCCGTACA AATGTACGGC CAGCAACGTC GGTTCGAGAT GGCGCTCGAT
      2161 GACGCCAACT ACCTCTGATA GTTGAGTCGA TACTTCGGCG ATCACCGCTT CCCTCATACT
      2221 CTTCCTTTTT CATTATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
      2281 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGCTAGCTCA CTCGGTCGCT ACGCTCCGGG
      2341 CGTGAGACTG CGGCGGGCGC TGCGGACACA TACAAAGTTA CCCACAGATT CCGTGGATAA
      2401 GCAGGGGACT AACATGTGAG GCAAAACAGC AGGGCCGCGC CGGTGGCGTT TTTCCATAGG
      2461 CTCCGCCCTC CTGCCAGAGT TCACATAAAC AGACGCTTTT CCGGTGCATC TGTGGGAGCC
      2521 GTGAGGCTCA ACCATGAATC TGACAGTACG GGCGAAACCC GACAGGACTT AAAGATCCCC
      2581 ACCGTTTCCG GCGGGTCGCT CCCTCTTGCG CTCTCCTGTT CCGACCCTGC CGTTTACCGG
      2641 ATACCTGTTC CGCCTTTCTC CCTTACGGGA AGTGTGGCGC TTTCTCATAG CTCACACACT
      2701 GGTATCTCGG CTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTAAGCAA GAACTCCCCG
      2761 TTCAGCCCGA CTGCTGCGCC TTATCCGGTA ACTGTTCACT TGAGTCCAAC CCGGAAAAGC
      2821 ACGGTAAAAC GCCACTGGCA GCAGCCATTG GTAACTGGGA GTTCGCAGAG GATTTGTTTA
      2881 GCTAAACACG CGGTTGCTCT TGAAGTGTGC GCCAAAGTCC GGCTACACTG GAAGGACAGA
      2941 TTTGGTTGCT GTGCTCTGCG AAAGCCAGTT ACCACGGTTA AGCAGTTCCC CAACTGACTT
      3001 AACCTTCGAT CAAACCACCT CCCCAGGTGG TTTTTTCGTT TACAGGGCAA AAGATTACGC
      3061 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACTGAACCG CTCTAGATTT
      3121 CAGTGCAATT TATCTCTTCA AATGTAGCAC CTGAAGTCAG CCCCATACGA TATAAGTTGT
      3181 AATTCTCATG TTAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT
      3241 CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT
      3301 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG
      3361 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA
      3421 GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC
      3481 GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA
      3541 TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATGTCC GCACCAACGC
      3601 GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA
      3661 GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA
      3721 TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT
      3781 TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG
      3841 CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT
      3901 GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA
      3961 CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA
      4021 TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC
      4081 CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA
      4141 TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA
      4201 GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG
      4261 CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA
      4321 CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG
      4381 GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA
      4441 AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC
      4501 ATTAGGAAAT TAATACGACT CACTATA
//