FEATURES             Location/Qualifiers
     misc_feature    complement(245..265)
                     /note="LIC Site"
     Region          complement(208..225)
                     /note="His Tag"
     promoter        complement(1492..1508)
                     /note="T7 Promoter"
     Region          complement(140..157)
                     /note="His Tag;carboxy terminal His tag from pET-21a"
     terminator      complement(26..72)
                     /note="T7 Terminator"
     CDS             2022..2981
                     /note="Lac I cds"
     CDS             complement(5170..6030)
                     /note="bla cds"
     rep_origin      4412..4412
                     /note="pBR322 origin of replication"
     misc_feature    complement(232..252)
                     /note="TEV site"
     misc_feature    complement(263..283)
                     /note="TVMV site"
     misc_feature    complement(314..1420)
                     /note="MBP cds"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTAATG ATGATGATGG TGGTGCCCGG CGGATTGGAA
       241 GTACAGGTTC TCCCCGGGAG AGACTCTGGA AACGCACGGT TTCCGAGCCT GCTTTTTTGT
       301 ACAAACTTGT GATCGAATTA GTCTGCGCGT CTTTCAGGGC TTCATCGACA GTCTGACGAC
       361 CGCTGGCGGC GTTGATCACC GCAGTACGCA CGGCATACCA GAAAGCGGAC ATCTGCGGGA
       421 TGTTCGGCAT GATTTCACCT TTCTGGGCGT TTTCCATGGT GGCGGCAATA CGTGGATCTT
       481 TCGCCAACTC TTCCTCGTAA GACTTCAGCG CTACGGCACC CAGCGGTTTG TCTTTATTAA
       541 CCGCTTCCAG ACCTTCATCA GTCAGCAGAT AGTTTTCGAG GAACTCTTTT GCCAGCTCTT
       601 TGTTCGGACT GGCGGCGTTA ATACCTGCGC TCAGCACGCC AACGAACGGT TTGGATGGTT
       661 GACCCTTGAA GGTCGGCAGT ACCGTTACAC CATAATTCAC TTTGCTGGTG TCGATGTTGG
       721 ACCATGCCCA CGGGCCGTTG ATGGTCATCG CTGTTTCGCC TTTATTAAAG GCAGCTTCTG
       781 CGATGGAGTA ATCGGTGTCT GCATTCATGT GTTTGTTTTT AATCAGGTCA ACCAGGAAGG
       841 TCAGACCCGC TTTCGCGCCA GCGTTATCCA CGCCCACGTC TTTAATGTCG TACTTGCCGT
       901 TTTCATACTT GAACGCATAA CCCCCGTCAG CAGCAATCAG CGGCCAGGTG AAGTACGGTT
       961 CTTGCAGGTT GAACATCAGC GCGCTCTTAC CTTTCGCTTT CAGTTCTTTA TCCAGCGCCG
      1021 GGATCTCTTC CCAGGTTTTT GGCGGGTTCG GCAGCAGATC TTTGTTATAA ATCAGCGATA
      1081 ACGCTTCAAC AGCGATCGGG TAAGCAATCA GCTTGCCGTT GTAACGTACG GCATCCCAGG
      1141 TAAACGGATA CAGCTTGTCC TGGAACGCTT TGTCCGGGGT GATTTCAGCC AACAGGCCAG
      1201 ATTGAGCGTA GCCACCAAAG CGGTCGTGTG CCCAGAAGAT AATGTCAGGG CCATCGCCAG
      1261 TTGCCGCAAC CTGTGGGAAT TTCTCTTCCA GTTTATCCGG ATGCTCAACG GTGACTTTAA
      1321 TTCCGGTATC TTTCTCGAAT TTCTTACCGA CTTCAGCGAG ACCGTTATAG CCTTTATCGC
      1381 CGTTAATCCA GATTACCAGT TTACCTTCTT CGATTTTCAT ATGTATATCT CCTTCTTAAA
      1441 GTTAAACAAA ATTATTTCTA GAGGGGAATT GTTATCCGCT CACAATTCCC CTATAGTGAG
      1501 TCGTATTAAT TTCGCGGGAT CGAGATCGAT CTCGATCCTC TACGCCGGAC GCATCGTGGC
      1561 CGGCATCACC GGCGCCACAG GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG
      1621 GGAAGATCGG GCTCGCCACT TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC
      1681 AGGCCCCGTG GCCGGGGGAC TGTTGGGCGC CATCTCCTTG CATGCACCAT TCCTTGCGGC
      1741 GGCGGTGCTC AACGGCCTCA ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA
      1801 GGGAGAGCGT CGAGATCCCG GACACCATCG AATGGCGCAA AACCTTTCGC GGTATGGCAT
      1861 GATAGCGCCC GGAAGAGAGT CAATTCAGGG TGGTGAATGT GAAACCAGTA ACGTTATACG
      1921 ATGTCGCAGA GTATGCCGGT GTCTCTTATC AGACCGTTTC CCGCGTGGTG AACCAGGCCA
      1981 GCCACGTTTC TGCGAAAACG CGGGAAAAAG TGGAAGCGGC GATGGCGGAG CTGAATTACA
      2041 TTCCCAACCG CGTGGCACAA CAACTGGCGG GCAAACAGTC GTTGCTGATT GGCGTTGCCA
      2101 CCTCCAGTCT GGCCCTGCAC GCGCCGTCGC AAATTGTCGC GGCGATTAAA TCTCGCGCCG
      2161 ATCAACTGGG TGCCAGCGTG GTGGTGTCGA TGGTAGAACG AAGCGGCGTC GAAGCCTGTA
      2221 AAGCGGCGGT GCACAATCTT CTCGCGCAAC GCGTCAGTGG GCTGATCATT AACTATCCGC
      2281 TGGATGACCA GGATGCCATT GCTGTGGAAG CTGCCTGCAC TAATGTTCCG GCGTTATTTC
      2341 TTGATGTCTC TGACCAGACA CCCATCAACA GTATTATTTT CTCCCATGAA GACGGTACGC
      2401 GACTGGGCGT GGAGCATCTG GTCGCATTGG GTCACCAGCA AATCGCGCTG TTAGCGGGCC
      2461 CATTAAGTTC TGTCTCGGCG CGTCTGCGTC TGGCTGGCTG GCATAAATAT CTCACTCGCA
      2521 ATCAAATTCA GCCGATAGCG GAACGGGAAG GCGACTGGAG TGCCATGTCC GGTTTTCAAC
      2581 AAACCATGCA AATGCTGAAT GAGGGCATCG TTCCCACTGC GATGCTGGTT GCCAACGATC
      2641 AGATGGCGCT GGGCGCAATG CGCGCCATTA CCGAGTCCGG GCTGCGCGTT GGTGCGGATA
      2701 TCTCGGTAGT GGGATACGAC GATACCGAAG ACAGCTCATG TTATATCCCG CCGTTAACCA
      2761 CCATCAAACA GGATTTTCGC CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT
      2821 CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCCGT CTCACTGGTG AAAAGAAAAA
      2881 CCACCCTGGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC
      2941 AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTA
      3001 AGTTAGCTCA CTCATTAGGC ACCGGGATCT CGACCGATGC CCTTGAGAGC CTTCAACCCA
      3061 GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTATCGTCG CCGCACTTAT GACTGTCTTC
      3121 TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT GGGTCATTTT CGGCGAGGAC
      3181 CGCTTTCGCT GGAGCGCGAC GATGATCGGC CTGTCGCTTG CGGTATTCGG AATCTTGCAC
      3241 GCCCTCGCTC AAGCCTTCGT CACTGGTCCC GCCACCAAAC GTTTCGGCGA GAAGCAGGCC
      3301 ATTATCGCCG GCATGGCGGC CCCACGGGTG CGCATGATCG TGCTCCTGTC GTTGAGGACC
      3361 CGGCTAGGCT GGCGGGGTTG CCTTACTGGT TAGCAGAATG AATCACCGAT ACGCGAGCGA
      3421 ACGTGAAGCG ACTGCTGCTG CAAAACGTCT GCGACCTGAG CAACAACATG AATGGTCTTC
      3481 GGTTTCCGTG TTTCGTAAAG TCTGGAAACG CGGAAGTCAG CGCCCTGCAC CATTATGTTC
      3541 CGGATCTGCA TCGCAGGATG CTGCTGGCTA CCCTGTGGAA CACCTACATC TGTATTAACG
      3601 AAGCGCTGGC ATTGACCCTG AGTGATTTTT CTCTGGTCCC GCCGCATCCA TACCGCCAGT
      3661 TGTTTACCCT CACAACGTTC CAGTAACCGG GCATGTTCAT CATCAGTAAC CCGTATCGTG
      3721 AGCATCCTCT CTCGTTTCAT CGGTATCATT ACCCCCATGA ACAGAAATCC CCCTTACACG
      3781 GAGGCATCAG TGACCAAACA GGAAAAAACC GCCCTTAACA TGGCCCGCTT TATCAGAAGC
      3841 CAGACATTAA CGCTTCTGGA GAAACTCAAC GAGCTGGACG CGGATGAACA GGCAGACATC
      3901 TGTGAATCGC TTCACGACCA CGCTGATGAG CTTTACCGCA GCTGCCTCGC GCGTTTCGGT
      3961 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA
      4021 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG
      4081 GGCGCAGCCA TGACCCAGTC ACGTAGCGAT AGCGGAGTGT ATACTGGCTT AACTATGCGG
      4141 CATCAGAGCA GATTGTACTG AGAGTGCACC ATATATGCGG TGTGAAATAC CGCACAGATG
      4201 CGTAAGGAGA AAATACCGCA TCAGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG
      4261 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC
      4321 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
      4381 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA
      4441 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
      4501 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
      4561 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
      4621 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
      4681 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
      4741 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
      4801 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT
      4861 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
      4921 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
      4981 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
      5041 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA
      5101 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG
      5161 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG
      5221 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC
      5281 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC
      5341 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC
      5401 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG
      5461 TTTGCGCAAC GTTGTTGCCA TTGCTGCAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT
      5521 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG
      5581 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT
      5641 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG
      5701 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG
      5761 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT
      5821 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT
      5881 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC
      5941 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT
      6001 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAAATTA TTGAAGCATT
      6061 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
      6121 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTA AATTGTAAGC GTTAATGTGA
      6181 ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC
      6241 TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA
      6301 AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG
      6361 CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCCC ATTCGCCA
//