FEATURES             Location/Qualifiers
     misc_feature    complement(245..265)
                     /note="LIC Site"
     Region          complement(208..225)
                     /note="His Tag"
     promoter        complement(1531..1547)
                     /note="T7 Promoter"
     terminator      complement(26..72)
                     /note="T7 Terminator"
     CDS             2061..3020
                     /note="Lac I cds"
     CDS             complement(5209..6069)
                     /note="bla cds"
     rep_origin      4451..4451
                     /note="pBR322 origin of replication"
     misc_feature    complement(232..252)
                     /note="TEV site"
     misc_feature    complement(263..283)
                     /note="TEV site"
     misc_feature    complement(305..1402)
                     /note="MBP cds"
     misc_feature    complement(1427..1456)
                     /note="His10"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTAATG ATGATGATGG TGGTGCCCGG CGGATTGGAA
       241 GTACAGGTTC TCCCCGGGAG AGACTTTGGA AGTACAGGTT CTCGGTACCT GGGATATCGT
       301 AATCAGTCTG CGCGTCTTTC AGGGCTTCAT CGACAGTCTG ACGACCGCTG GCGGCGTTGA
       361 TCACCGCAGT ACGCACGGCA TACCAGAAAG CGGACATCTG CGGGATGTTC GGCATGATTT
       421 CACCTTTCTG GGCGTTTTCC ATGGTGGCGG CAATACGTGG ATCTTTCGCC AACTCTTCCT
       481 CGTAAGACTT CAGCGCTACG GCACCCAGCG GTTTGTCTTT ATTAACCGCT TCCAGACCTT
       541 CATCAGTCAG CAGATAGTTT TCGAGGAACT CTTTTGCCAG CTCTTTGTTC GGACTGGCGG
       601 CGTTAATACC TGCGCTCAGC ACGCCAACGA ACGGTTTGGA TGGTTGACCC TTGAAGGTCG
       661 GCAGTACCGT TACACCATAA TTCACTTTGC TGGTGTCGAT GTTGGACCAT GCCCACGGGC
       721 CGTTGATGGT CATCGCTGTT TCGCCTTTAT TAAAGGCAGC TTCTGCGATG GAGTAATCGG
       781 TGTCTGCATT CATGTGTTTG TTTTTAATCA GGTCAACCAG GAAGGTCAGA CCCGCTTTCG
       841 CGCCAGCGTT ATCCACGCCC ACGTCTTTAA TGTCGTACTT GCCGTTTTCA TACTTGAACG
       901 CATAACCCCC GTCAGCAGCA ATCAGCGGCC AGGTGAAGTA CGGTTCTTGC AGGTTGAACA
       961 TCAGCGCGCT CTTACCTTTC GCTTTCAGTT CTTTATCCAG CGCCGGGATC TCTTCCCAGG
      1021 TTTTTGGCGG GTTCGGCAGC AGATCTTTGT TATAAATCAG CGATAACGCT TCAACAGCGA
      1081 TCGGGTAAGC AATCAGCTTG CCGTTGTAAC GTACGGCATC CCAGGTAAAC GGATACAGCT
      1141 TGTCCTGGAA CGCTTTGTCC GGGGTGATTT CAGCCAACAG GCCAGATTGA GCGTAGCCAC
      1201 CAAAGCGGTC GTGTGCCCAG AAGATAATGT CAGGGCCATC GCCAGTTGCC GCAACCTGTG
      1261 GGAATTTCTC TTCCAGTTTA TCCGGATGCT CAACGGTGAC TTTAATTCCG GTATCTTTCT
      1321 CGAATTTCTT ACCGACTTCA GCGAGACCGT TATAGCCTTT ATCGCCGTTA ATCCAGATTA
      1381 CCAGTTTACC TTCTTCGATT TTGGTACCCA GATCTACACC AGAAGAGTGG TGGTGGTGAT
      1441 GATGATGATG ATGGTGCATA TGTATATCTC CTTCTTAAAG TTAAACAAAA TTATTTCTAG
      1501 AGGGGAATTG TTATCCGCTC ACAATTCCCC TATAGTGAGT CGTATTAATT TCGCGGGATC
      1561 GAGATCGATC TCGATCCTCT ACGCCGGACG CATCGTGGCC GGCATCACCG GCGCCACAGG
      1621 TGCGGTTGCT GGCGCCTATA TCGCCGACAT CACCGATGGG GAAGATCGGG CTCGCCACTT
      1681 CGGGCTCATG AGCGCTTGTT TCGGCGTGGG TATGGTGGCA GGCCCCGTGG CCGGGGGACT
      1741 GTTGGGCGCC ATCTCCTTGC ATGCACCATT CCTTGCGGCG GCGGTGCTCA ACGGCCTCAA
      1801 CCTACTACTG GGCTGCTTCC TAATGCAGGA GTCGCATAAG GGAGAGCGTC GAGATCCCGG
      1861 ACACCATCGA ATGGCGCAAA ACCTTTCGCG GTATGGCATG ATAGCGCCCG GAAGAGAGTC
      1921 AATTCAGGGT GGTGAATGTG AAACCAGTAA CGTTATACGA TGTCGCAGAG TATGCCGGTG
      1981 TCTCTTATCA GACCGTTTCC CGCGTGGTGA ACCAGGCCAG CCACGTTTCT GCGAAAACGC
      2041 GGGAAAAAGT GGAAGCGGCG ATGGCGGAGC TGAATTACAT TCCCAACCGC GTGGCACAAC
      2101 AACTGGCGGG CAAACAGTCG TTGCTGATTG GCGTTGCCAC CTCCAGTCTG GCCCTGCACG
      2161 CGCCGTCGCA AATTGTCGCG GCGATTAAAT CTCGCGCCGA TCAACTGGGT GCCAGCGTGG
      2221 TGGTGTCGAT GGTAGAACGA AGCGGCGTCG AAGCCTGTAA AGCGGCGGTG CACAATCTTC
      2281 TCGCGCAACG CGTCAGTGGG CTGATCATTA ACTATCCGCT GGATGACCAG GATGCCATTG
      2341 CTGTGGAAGC TGCCTGCACT AATGTTCCGG CGTTATTTCT TGATGTCTCT GACCAGACAC
      2401 CCATCAACAG TATTATTTTC TCCCATGAAG ACGGTACGCG ACTGGGCGTG GAGCATCTGG
      2461 TCGCATTGGG TCACCAGCAA ATCGCGCTGT TAGCGGGCCC ATTAAGTTCT GTCTCGGCGC
      2521 GTCTGCGTCT GGCTGGCTGG CATAAATATC TCACTCGCAA TCAAATTCAG CCGATAGCGG
      2581 AACGGGAAGG CGACTGGAGT GCCATGTCCG GTTTTCAACA AACCATGCAA ATGCTGAATG
      2641 AGGGCATCGT TCCCACTGCG ATGCTGGTTG CCAACGATCA GATGGCGCTG GGCGCAATGC
      2701 GCGCCATTAC CGAGTCCGGG CTGCGCGTTG GTGCGGATAT CTCGGTAGTG GGATACGACG
      2761 ATACCGAAGA CAGCTCATGT TATATCCCGC CGTTAACCAC CATCAAACAG GATTTTCGCC
      2821 TGCTGGGGCA AACCAGCGTG GACCGCTTGC TGCAACTCTC TCAGGGCCAG GCGGTGAAGG
      2881 GCAATCAGCT GTTGCCCGTC TCACTGGTGA AAAGAAAAAC CACCCTGGCG CCCAATACGC
      2941 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC
      3001 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTAA GTTAGCTCAC TCATTAGGCA
      3061 CCGGGATCTC GACCGATGCC CTTGAGAGCC TTCAACCCAG TCAGCTCCTT CCGGTGGGCG
      3121 CGGGGCATGA CTATCGTCGC CGCACTTATG ACTGTCTTCT TTATCATGCA ACTCGTAGGA
      3181 CAGGTGCCGG CAGCGCTCTG GGTCATTTTC GGCGAGGACC GCTTTCGCTG GAGCGCGACG
      3241 ATGATCGGCC TGTCGCTTGC GGTATTCGGA ATCTTGCACG CCCTCGCTCA AGCCTTCGTC
      3301 ACTGGTCCCG CCACCAAACG TTTCGGCGAG AAGCAGGCCA TTATCGCCGG CATGGCGGCC
      3361 CCACGGGTGC GCATGATCGT GCTCCTGTCG TTGAGGACCC GGCTAGGCTG GCGGGGTTGC
      3421 CTTACTGGTT AGCAGAATGA ATCACCGATA CGCGAGCGAA CGTGAAGCGA CTGCTGCTGC
      3481 AAAACGTCTG CGACCTGAGC AACAACATGA ATGGTCTTCG GTTTCCGTGT TTCGTAAAGT
      3541 CTGGAAACGC GGAAGTCAGC GCCCTGCACC ATTATGTTCC GGATCTGCAT CGCAGGATGC
      3601 TGCTGGCTAC CCTGTGGAAC ACCTACATCT GTATTAACGA AGCGCTGGCA TTGACCCTGA
      3661 GTGATTTTTC TCTGGTCCCG CCGCATCCAT ACCGCCAGTT GTTTACCCTC ACAACGTTCC
      3721 AGTAACCGGG CATGTTCATC ATCAGTAACC CGTATCGTGA GCATCCTCTC TCGTTTCATC
      3781 GGTATCATTA CCCCCATGAA CAGAAATCCC CCTTACACGG AGGCATCAGT GACCAAACAG
      3841 GAAAAAACCG CCCTTAACAT GGCCCGCTTT ATCAGAAGCC AGACATTAAC GCTTCTGGAG
      3901 AAACTCAACG AGCTGGACGC GGATGAACAG GCAGACATCT GTGAATCGCT TCACGACCAC
      3961 GCTGATGAGC TTTACCGCAG CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA
      4021 CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA
      4081 GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA
      4141 CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA
      4201 GAGTGCACCA TATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT
      4261 CAGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG
      4321 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC
      4381 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT
      4441 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
      4501 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
      4561 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
      4621 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
      4681 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
      4741 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
      4801 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
      4861 GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA
      4921 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
      4981 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
      5041 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
      5101 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
      5161 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
      5221 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
      5281 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
      5341 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
      5401 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
      5461 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
      5521 TGCTGCAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
      5581 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
      5641 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
      5701 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
      5761 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
      5821 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
      5881 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA
      5941 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG
      6001 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG
      6061 AATACTCATA CTCTTCCTTT TTCAAATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
      6121 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT
      6181 CCCCGAAAAG TGCCACCTAA ATTGTAAGCG TTAATGTGAA CCATCACCCT AATCAAGTTT
      6241 TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG
      6301 AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC
      6361 GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC
      6421 GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCA
//