FEATURES             Location/Qualifiers
     CDS             complement(726..1436)
                     /note="TVMV Protease"
     promoter        complement(1455..1610)
                     /note="pLUX I - Tet 0"
     misc_feature    204..235
                     /note="LIC Site"
     Cleavage-site   223..243
                     /note="TEV Site"
     Region          268..285
                     /note="His Tag"
     promoter        complement(360..376)
                     /note="T7 Promoter"
     terminator      complement(26..72)
                     /note="T7 Terminator"
     CDS             1917..2876
                     /note="Lac I cds"
     CDS             complement(5065..5925)
                     /note="bla cds"
     rep_origin      4307..4307
                     /note="pBR322 origin of replication"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTATCC ACTTCCAATA TTGGATTGGA AGTACAGGTT
       241 CTCGGTACCC AGATCTACAC CAGAAGAATG ATGATGATGA TGGTGCATAT GTATATCTCC
       301 TTCTTAAAGT TAAACAAAAT TATTTCTAGA GGGGAATTGT TATCCGCTCA CAATTCCCCT
       361 ATAGTGAGTC GTATTAATTT CGCGGGATCG AGATCGATCT CGATCCTCTA CGCCGGACGC
       421 ATCGTGGCCG GCATCACCGG CGCCACAGGT GCGGTTGCTG GCGCCTATAT CGCCGACATC
       481 ACCGATGGGG AAGATCGGGC TCGCCACTTC GGGCTCATGA GCGCTTGTTT CGGCGTGGGT
       541 ATGGTGGCAG GCCCCGTGGC CGGGGGACTG TTGGGCGCCA TCTCCTTGCA TGCTCTAGGG
       601 CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA CAACAGATAA AACGAAAGGC
       661 CCAGTCTTTC GACTGAGCCT TTCGTTTTAT TTGATGCCTC TAGCACGCGT ACCATGGGAT
       721 CTTTATTAGT CCATGATGGC GGCAACAGTT TTTTTGGCCA TGAAGTCATC TTCCGGCGCA
       781 TCTTCAACCA GGGTAAAGGA ACCCCAGCTG ATTTTATCCG CGTTGAATTT CCAGTTTTTG
       841 CACCAACCAT CCGCGGCATC TAGATAAGTC GCCACGAATT TTTCCGGAAA TTCCACGAAG
       901 TAGTTGCTAC CGTTGGTGGT ATGAGTCAGG CTGTGGATGC CCAGAATGTT GCCATCAATG
       961 ATGGAAACTA GTGGGCTGCC ACACTGGCCA TCTTTAGTGG TGATCCAGTG CTGCCAGAAA
      1021 GAAGTGTCTT CTTTATGCAC AATGTGAGAG GATTCAGACA CCAGGCTCGA GACGCTTTTC
      1081 TGCTGAAAGT TGGTGGACAC CATGCACACA CGATCTTTGA TGGTCGGCTG ACGGAATTTC
      1141 AGTTTCTGCG GGAACGGCGG GAAGTCTTTA GCCATTTTGA TAACGATAAT GTCACGGCCT
      1201 TCAACCGGTT TCATCTGCAG CTGGGTAGAG TTTTTGACTT TGAATTCACC ATGCATGGTT
      1261 TTGATGGTCA GTTCGCCATT GTTACGACGA AACAGATGCT GGTTGGCAAT GATATACGGG
      1321 CCAAAACCAA TGCCAAACAG ACGTTCACTA TGACCATCCG AGGAGTTTTC CAGCAGGCAT
      1381 ACGCAAGCAG AGATCGGATT AAAATCGCGC ACGCCCTTCA GCAAAGCTTT AGACATATGT
      1441 TACCTCCTTA AATCAATTCG GTCAGTGCGT CCTGCTGATG TGCTCAGTAT CTCTATCACT
      1501 GATAGGGATG TCAATCTCTA TCACTGATAG GGACTCGAGG TGAAGACGAA AGGGCCTCGT
      1561 GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCGCATGC
      1621 ACCATTCCTT GCGGCGGCGG TGCTCAACGG CCTCAACCTA CTACTGGGCT GCTTCCTAAT
      1681 GCAGGAGTCG CATAAGGGAG AGCGTCGAGA TCCCGGACAC CATCGAATGG CGCAAAACCT
      1741 TTCGCGGTAT GGCATGATAG CGCCCGGAAG AGAGTCAATT CAGGGTGGTG AATGTGAAAC
      1801 CAGTAACGTT ATACGATGTC GCAGAGTATG CCGGTGTCTC TTATCAGACC GTTTCCCGCG
      1861 TGGTGAACCA GGCCAGCCAC GTTTCTGCGA AAACGCGGGA AAAAGTGGAA GCGGCGATGG
      1921 CGGAGCTGAA TTACATTCCC AACCGCGTGG CACAACAACT GGCGGGCAAA CAGTCGTTGC
      1981 TGATTGGCGT TGCCACCTCC AGTCTGGCCC TGCACGCGCC GTCGCAAATT GTCGCGGCGA
      2041 TTAAATCTCG CGCCGATCAA CTGGGTGCCA GCGTGGTGGT GTCGATGGTA GAACGAAGCG
      2101 GCGTCGAAGC CTGTAAAGCG GCGGTGCACA ATCTTCTCGC GCAACGCGTC AGTGGGCTGA
      2161 TCATTAACTA TCCGCTGGAT GACCAGGATG CCATTGCTGT GGAAGCTGCC TGCACTAATG
      2221 TTCCGGCGTT ATTTCTTGAT GTCTCTGACC AGACACCCAT CAACAGTATT ATTTTCTCCC
      2281 ATGAAGACGG TACGCGACTG GGCGTGGAGC ATCTGGTCGC ATTGGGTCAC CAGCAAATCG
      2341 CGCTGTTAGC GGGCCCATTA AGTTCTGTCT CGGCGCGTCT GCGTCTGGCT GGCTGGCATA
      2401 AATATCTCAC TCGCAATCAA ATTCAGCCGA TAGCGGAACG GGAAGGCGAC TGGAGTGCCA
      2461 TGTCCGGTTT TCAACAAACC ATGCAAATGC TGAATGAGGG CATCGTTCCC ACTGCGATGC
      2521 TGGTTGCCAA CGATCAGATG GCGCTGGGCG CAATGCGCGC CATTACCGAG TCCGGGCTGC
      2581 GCGTTGGTGC GGATATCTCG GTAGTGGGAT ACGACGATAC CGAAGACAGC TCATGTTATA
      2641 TCCCGCCGTT AACCACCATC AAACAGGATT TTCGCCTGCT GGGGCAAACC AGCGTGGACC
      2701 GCTTGCTGCA ACTCTCTCAG GGCCAGGCGG TGAAGGGCAA TCAGCTGTTG CCCGTCTCAC
      2761 TGGTGAAAAG AAAAACCACC CTGGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG
      2821 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC
      2881 AACGCAATTA ATGTAAGTTA GCTCACTCAT TAGGCACCGG GATCTCGACC GATGCCCTTG
      2941 AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG GCATGACTAT CGTCGCCGCA
      3001 CTTATGACTG TCTTCTTTAT CATGCAACTC GTAGGACAGG TGCCGGCAGC GCTCTGGGTC
      3061 ATTTTCGGCG AGGACCGCTT TCGCTGGAGC GCGACGATGA TCGGCCTGTC GCTTGCGGTA
      3121 TTCGGAATCT TGCACGCCCT CGCTCAAGCC TTCGTCACTG GTCCCGCCAC CAAACGTTTC
      3181 GGCGAGAAGC AGGCCATTAT CGCCGGCATG GCGGCCCCAC GGGTGCGCAT GATCGTGCTC
      3241 CTGTCGTTGA GGACCCGGCT AGGCTGGCGG GGTTGCCTTA CTGGTTAGCA GAATGAATCA
      3301 CCGATACGCG AGCGAACGTG AAGCGACTGC TGCTGCAAAA CGTCTGCGAC CTGAGCAACA
      3361 ACATGAATGG TCTTCGGTTT CCGTGTTTCG TAAAGTCTGG AAACGCGGAA GTCAGCGCCC
      3421 TGCACCATTA TGTTCCGGAT CTGCATCGCA GGATGCTGCT GGCTACCCTG TGGAACACCT
      3481 ACATCTGTAT TAACGAAGCG CTGGCATTGA CCCTGAGTGA TTTTTCTCTG GTCCCGCCGC
      3541 ATCCATACCG CCAGTTGTTT ACCCTCACAA CGTTCCAGTA ACCGGGCATG TTCATCATCA
      3601 GTAACCCGTA TCGTGAGCAT CCTCTCTCGT TTCATCGGTA TCATTACCCC CATGAACAGA
      3661 AATCCCCCTT ACACGGAGGC ATCAGTGACC AAACAGGAAA AAACCGCCCT TAACATGGCC
      3721 CGCTTTATCA GAAGCCAGAC ATTAACGCTT CTGGAGAAAC TCAACGAGCT GGACGCGGAT
      3781 GAACAGGCAG ACATCTGTGA ATCGCTTCAC GACCACGCTG ATGAGCTTTA CCGCAGCTGC
      3841 CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC
      3901 ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT
      3961 GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA GCGATAGCGG AGTGTATACT
      4021 GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATA TGCGGTGTGA
      4081 AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCTCTTCCG CTTCCTCGCT
      4141 CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC
      4201 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG
      4261 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG
      4321 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
      4381 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
      4441 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
      4501 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
      4561 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
      4621 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
      4681 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
      4741 TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
      4801 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
      4861 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
      4921 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
      4981 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
      5041 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
      5101 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
      5161 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC
      5221 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
      5281 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
      5341 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT GCAGGCATCG TGGTGTCACG
      5401 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
      5461 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
      5521 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
      5581 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
      5641 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
      5701 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
      5761 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
      5821 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
      5881 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
      5941 AATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
      6001 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTAAATTG
      6061 TAAGCGTTAA TGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC
      6121 ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA
      6181 CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT
      6241 AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC
      6301 GTCCCATTCG CCA
//