FEATURES Location/Qualifiers misc_feature complement(245..265) /note="LIC Site" Region complement(208..225) /note="His Tag" promoter complement(1492..1508) /note="T7 Promoter" Region complement(140..157) /note="His Tag;carboxy terminal His tag from pET-21a" terminator complement(26..72) /note="T7 Terminator" CDS 3049..4008 /note="Lac I cds" CDS complement(6197..7057) /note="bla cds" rep_origin 5439..5439 /note="pBR322 origin of replication" misc_feature complement(232..252) /note="TEV site" misc_feature complement(263..283) /note="TVMV site" misc_feature complement(314..1420) /note="MBP cds" CDS 1904..2614 /note="TVMV protease cds" 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT 121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT 181 CGACGGAGCT CGAATTCGGA TCCGTTAATG ATGATGATGG TGGTGCCCGG CGGATTGGAA 241 GTACAGGTTC TCCCCGGGAG AGACTCTGGA AACGCACGGT TTCCGAGCCT GCTTTTTTGT 301 ACAAACTTGT GATCGAATTA GTCTGCGCGT CTTTCAGGGC TTCATCGACA GTCTGACGAC 361 CGCTGGCGGC GTTGATCACC GCAGTACGCA CGGCATACCA GAAAGCGGAC ATCTGCGGGA 421 TGTTCGGCAT GATTTCACCT TTCTGGGCGT TTTCCATGGT GGCGGCAATA CGTGGATCTT 481 TCGCCAACTC TTCCTCGTAA GACTTCAGCG CTACGGCACC CAGCGGTTTG TCTTTATTAA 541 CCGCTTCCAG ACCTTCATCA GTCAGCAGAT AGTTTTCGAG GAACTCTTTT GCCAGCTCTT 601 TGTTCGGACT GGCGGCGTTA ATACCTGCGC TCAGCACGCC AACGAACGGT TTGGATGGTT 661 GACCCTTGAA GGTCGGCAGT ACCGTTACAC CATAATTCAC TTTGCTGGTG TCGATGTTGG 721 ACCATGCCCA CGGGCCGTTG ATGGTCATCG CTGTTTCGCC TTTATTAAAG GCAGCTTCTG 781 CGATGGAGTA ATCGGTGTCT GCATTCATGT GTTTGTTTTT AATCAGGTCA ACCAGGAAGG 841 TCAGACCCGC TTTCGCGCCA GCGTTATCCA CGCCCACGTC TTTAATGTCG TACTTGCCGT 901 TTTCATACTT GAACGCATAA CCCCCGTCAG CAGCAATCAG CGGCCAGGTG AAGTACGGTT 961 CTTGCAGGTT GAACATCAGC GCGCTCTTAC CTTTCGCTTT CAGTTCTTTA TCCAGCGCCG 1021 GGATCTCTTC CCAGGTTTTT GGCGGGTTCG GCAGCAGATC TTTGTTATAA ATCAGCGATA 1081 ACGCTTCAAC AGCGATCGGG TAAGCAATCA GCTTGCCGTT GTAACGTACG GCATCCCAGG 1141 TAAACGGATA CAGCTTGTCC TGGAACGCTT TGTCCGGGGT GATTTCAGCC AACAGGCCAG 1201 ATTGAGCGTA GCCACCAAAG CGGTCGTGTG CCCAGAAGAT AATGTCAGGG CCATCGCCAG 1261 TTGCCGCAAC CTGTGGGAAT TTCTCTTCCA GTTTATCCGG ATGCTCAACG GTGACTTTAA 1321 TTCCGGTATC TTTCTCGAAT TTCTTACCGA CTTCAGCGAG ACCGTTATAG CCTTTATCGC 1381 CGTTAATCCA GATTACCAGT TTACCTTCTT CGATTTTCAT ATGTATATCT CCTTCTTAAA 1441 GTTAAACAAA ATTATTTCTA GAGGGGAATT GTTATCCGCT CACAATTCCC CTATAGTGAG 1501 TCGTATTAAT TTCGCGGGAT CGAGATCGAT CTCGATCCTC TACGCCGGAC GCATCGTGGC 1561 CGGCATCACC GGCGCCACAG GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG 1621 GGAAGATCGG GCTCGCCACT TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC 1681 AGGCCCCGTG GCCGGGGGAC TGTTGGGCGC CATCTCCTTG CATGCGACGT CTAAGAAACC 1741 ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTTCAC 1801 CTCGAGTCCC TATCAGTGAT AGAGATTGAC ATCCCTATCA GTGATAGAGA TACTGAGCAC 1861 ATCAGCAGGA CGCACTGACC GAATTGATTT AAGGAGGTAA CATATGTCTA AAGCTTTGCT 1921 GAAGGGCGTG CGCGATTTTA ATCCGATCTC TGCTTGCGTA TGCCTGCTGG AAAACTCCTC 1981 GGATGGTCAT AGTGAACGTC TGTTTGGCAT TGGTTTTGGC CCGTATATCA TTGCCAACCA 2041 GCATCTGTTT CGTCGTAACA ATGGCGAACT GACCATCAAA ACCATGCATG GTGAATTCAA 2101 AGTCAAAAAC TCTACCCAGC TGCAGATGAA ACCGGTTGAA GGCCGTGACA TTATCGTTAT 2161 CAAAATGGCT AAAGACTTCC CGCCGTTCCC GCAGAAACTG AAATTCCGTC AGCCGACCAT 2221 CAAAGATCGT GTGTGCATGG TGTCCACCAA CTTTCAGCAG AAAAGCGTCT CGAGCCTGGT 2281 GTCTGAATCC TCTCACATTG TGCATAAAGA AGACACTTCT TTCTGGCAGC ACTGGATCAC 2341 CACTAAAGAT GGCCAGTGTG GCAGCCCACT AGTTTCCATC ATTGATGGCA ACATTCTGGG 2401 CATCCACAGC CTGACTCATA CCACCAACGG TAGCAACTAC TTCGTGGAAT TTCCGGAAAA 2461 ATTCGTGGCG ACTTATCTAG ATGCCGCGGA TGGTTGGTGC AAAAACTGGA AATTCAACGC 2521 GGATAAAATC AGCTGGGGTT CCTTTACCCT GGTTGAAGAT GCGCCGGAAG ATGACTTCAT 2581 GGCCAAAAAA ACTGTTGCCG CCATCATGGA CTAATAAAGA TCCCATGGTA CGCGTGCTAG 2641 AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT 2701 TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGC CCTAGAGCAT GCACCATTCC 2761 TTGCGGCGGC GGTGCTCAAC GGCCTCAACC TACTACTGGG CTGCTTCCTA ATGCAGGAGT 2821 CGCATAAGGG AGAGCGTCGA GATCCCGGAC ACCATCGAAT GGCGCAAAAC CTTTCGCGGT 2881 ATGGCATGAT AGCGCCCGGA AGAGAGTCAA TTCAGGGTGG TGAATGTGAA ACCAGTAACG 2941 TTATACGATG TCGCAGAGTA TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC 3001 CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG 3061 AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA AACAGTCGTT GCTGATTGGC 3121 GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA TTGTCGCGGC GATTAAATCT 3181 CGCGCCGATC AACTGGGTGC CAGCGTGGTG GTGTCGATGG TAGAACGAAG CGGCGTCGAA 3241 GCCTGTAAAG CGGCGGTGCA CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC 3301 TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG 3361 TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA TTATTTTCTC CCATGAAGAC 3421 GGTACGCGAC TGGGCGTGGA GCATCTGGTC GCATTGGGTC ACCAGCAAAT CGCGCTGTTA 3481 GCGGGCCCAT TAAGTTCTGT CTCGGCGCGT CTGCGTCTGG CTGGCTGGCA TAAATATCTC 3541 ACTCGCAATC AAATTCAGCC GATAGCGGAA CGGGAAGGCG ACTGGAGTGC CATGTCCGGT 3601 TTTCAACAAA CCATGCAAAT GCTGAATGAG GGCATCGTTC CCACTGCGAT GCTGGTTGCC 3661 AACGATCAGA TGGCGCTGGG CGCAATGCGC GCCATTACCG AGTCCGGGCT GCGCGTTGGT 3721 GCGGATATCT CGGTAGTGGG ATACGACGAT ACCGAAGACA GCTCATGTTA TATCCCGCCG 3781 TTAACCACCA TCAAACAGGA TTTTCGCCTG CTGGGGCAAA CCAGCGTGGA CCGCTTGCTG 3841 CAACTCTCTC AGGGCCAGGC GGTGAAGGGC AATCAGCTGT TGCCCGTCTC ACTGGTGAAA 3901 AGAAAAACCA CCCTGGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA 3961 TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT 4021 TAATGTAAGT TAGCTCACTC ATTAGGCACC GGGATCTCGA CCGATGCCCT TGAGAGCCTT 4081 CAACCCAGTC AGCTCCTTCC GGTGGGCGCG GGGCATGACT ATCGTCGCCG CACTTATGAC 4141 TGTCTTCTTT ATCATGCAAC TCGTAGGACA GGTGCCGGCA GCGCTCTGGG TCATTTTCGG 4201 CGAGGACCGC TTTCGCTGGA GCGCGACGAT GATCGGCCTG TCGCTTGCGG TATTCGGAAT 4261 CTTGCACGCC CTCGCTCAAG CCTTCGTCAC TGGTCCCGCC ACCAAACGTT TCGGCGAGAA 4321 GCAGGCCATT ATCGCCGGCA TGGCGGCCCC ACGGGTGCGC ATGATCGTGC TCCTGTCGTT 4381 GAGGACCCGG CTAGGCTGGC GGGGTTGCCT TACTGGTTAG CAGAATGAAT CACCGATACG 4441 CGAGCGAACG TGAAGCGACT GCTGCTGCAA AACGTCTGCG ACCTGAGCAA CAACATGAAT 4501 GGTCTTCGGT TTCCGTGTTT CGTAAAGTCT GGAAACGCGG AAGTCAGCGC CCTGCACCAT 4561 TATGTTCCGG ATCTGCATCG CAGGATGCTG CTGGCTACCC TGTGGAACAC CTACATCTGT 4621 ATTAACGAAG CGCTGGCATT GACCCTGAGT GATTTTTCTC TGGTCCCGCC GCATCCATAC 4681 CGCCAGTTGT TTACCCTCAC AACGTTCCAG TAACCGGGCA TGTTCATCAT CAGTAACCCG 4741 TATCGTGAGC ATCCTCTCTC GTTTCATCGG TATCATTACC CCCATGAACA GAAATCCCCC 4801 TTACACGGAG GCATCAGTGA CCAAACAGGA AAAAACCGCC CTTAACATGG CCCGCTTTAT 4861 CAGAAGCCAG ACATTAACGC TTCTGGAGAA ACTCAACGAG CTGGACGCGG ATGAACAGGC 4921 AGACATCTGT GAATCGCTTC ACGACCACGC TGATGAGCTT TACCGCAGCT GCCTCGCGCG 4981 TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG 5041 TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG 5101 GTGTCGGGGC GCAGCCATGA CCCAGTCACG TAGCGATAGC GGAGTGTATA CTGGCTTAAC 5161 TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TATGCGGTGT GAAATACCGC 5221 ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT 5281 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 5341 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 5401 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 5461 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 5521 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 5581 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 5641 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 5701 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 5761 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 5821 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA 5881 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 5941 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 6001 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 6061 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 6121 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 6181 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 6241 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 6301 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 6361 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 6421 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 6481 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTGCAGGCAT CGTGGTGTCA CGCTCGTCGT 6541 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 6601 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 6661 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 6721 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 6781 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 6841 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 6901 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 6961 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 7021 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAAATTATTG 7081 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 7141 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTAAAT TGTAAGCGTT 7201 AATGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC 7261 GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA 7321 GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA 7381 CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTCCCATT 7441 CGCCA //