FEATURES             Location/Qualifiers
     Region          complement(305..907)
                     /note="osmY cds;osmY cds"
     CDS             1445..2071
                     /note="dsbA cds"
     promoter        1213..1444
                     /note="BL21 promoter"
     misc_feature    complement(245..265)
                     /note="LIC Site"
     Region          complement(208..225)
                     /note="His Tag"
     promoter        complement(1847..1863)
                     /note="T7 Promoter"
     terminator      complement(26..72)
                     /note="T7 Terminator"
     CDS             2377..3336
                     /note="Lac I cds"
     CDS             complement(5525..6385)
                     /note="bla cds"
     rep_origin      4767..4767
                     /note="pBR322 origin of replication"
     misc_feature    complement(232..252)
                     /note="TEV site"
     misc_feature    complement(263..283)
                     /note="TVMV site"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTAATG ATGATGATGG TGGTGCCCGG CGGATTGGAA
       241 GTACAGGTTC TCCCCGGGAG AGACTCTGGA AACGCACGGT TTCCGAGCCT GCTTTTTTGT
       301 ACAACTTAGT TTTCAGATCA TTTTTAACGC TTTTCACACC ATCTACCGCT TTGGCGATAC
       361 TTTCAGCACG GTCACTTTGT GCCTGAGAAT CGACGGTACC GGAGAGCTGA ACCACGCCGT
       421 CGGTGGTTTC AACTTTCACA TGACGGGAAG GGACGATATC GTCCGCCAGC AGTTTGGCTT
       481 TGATTTCACT GGTGGTGGCG GTGTCACCCG CGTAGCCCTT CACCGAGCCT TCTTTAGCGT
       541 CGCGAACGTG CAGTTTGTCG CTGACAGAGG TCACCCCTTC AACGCCTTTC GCCACTTTCA
       601 CTGCCTCTTC GGCCTGGGCC TGGCTTTCAA CGAAACCGCT CAGGGTCACG ACTTTTTGAT
       661 CGGTTTTTAC AGAGATATCG GTGCTCTTGA TGTTGTCATG ATCCACCAGG GCCGCCTTCA
       721 CTTTCGCGGT GATGGCGCTG TCATCCATGA AATTACCGAC TTTATTCATA GAGCTATCGA
       781 CTTTTTGCCC TGCGCTTTCA TTGGTAGTCT GCGCGTTGTT TTCCGCGTAG GCAGAGCCGG
       841 TCGCGACGGC AGAGGTCAAC ATTACAGCCA GCAGAGTTTT CGAAATCTTC AGTCTTGTCA
       901 TAGTCATATG TATATCTCCT TCTTAAAGTT AAACAAAATT ATTTCTAGAG GGGAATTGTT
       961 ATCCGCTCAC AATTCCCCTA TAGTGAGTCG TATTAATTTC GCGGGATCGA GATCGATCTC
      1021 GATCCTCTAC GCCGGACGCA TCGTGGCCGG CATCACCGGC GCCACAGGTG CGGTTGCTGG
      1081 CGCCTATATC GCCGACATCA CCGATGGGGA AGATCGGGCT CGCCACTTCG GGCTCATGAG
      1141 CGCTTGTTTC GGCGTGGGTA TGGTGGCAGG CCCCGTGGCC GGGGGACTGT TGGGCGCCAT
      1201 CTCCTTGCAT GCTTCGACAC CGCTGAAATC GGACTGATTG AACCTTTACG CGCCATGCGT
      1261 TTGGTTTATT ATCTTGCCTG GCTAATGCGG CGTTGGGCTG ATCCCGCGTT CCCGAAAAAT
      1321 TTCCCGTGGT TAACCGGGGA AGATTACTGG CTACGACAGA CGGCGACTTT TATAGAACAG
      1381 GCAAAAGTTC TACAAGAACC CCCTTTGCAA TTAACACCTA TGTATTAATC GGAGAGAGTA
      1441 GATCATGAAA AAGATTTGGC TGGCGCTGGC TGGTTTAGTT TTAGCGTTTA GCGCATCGGC
      1501 GGCGCAGTAT GAAGATGGTA AACAGTACAC TACCCTGGAA AAACCGGTAG CTGGCGCGCC
      1561 GCAAGTGCTG GAGTTTTTCT CTTTCTTCTG CCCGCACTGC TATCAGTTTG AAGAAGTTCT
      1621 GCATATTTCT GATAATGTGA AGAAAAAACT GCCGGAAGGC GTGAAGATGA CTAAATACCA
      1681 CGTCAACTTC ATGGGTGGTG ACCTGGGCAA AGATCTGACT CAGGCATGGG CTGTGGCGAT
      1741 GGCGCTGGGC GTGGAAGACA AAGTCACTGT TCCGCTGTTT GAAGGCGTAC AGAAAACCCA
      1801 GACCATTCGT TCAGCATCTG ATATCCGCGA TGTATTTATC AACGCAGGTA TTAAAGGTGA
      1861 AGAGTACGAC GCGGCGTGGA ACAGCTTCGT GGTCAAATCT CTGGTCGCTC AGCAGGAAAA
      1921 AGCTGCAGCT GACGTGCAAT TGCGTGGCGT TCCGGCGATG TTTGTTAACG GTAAATATCA
      1981 GCTGAATCCG CAGGGTATGG ATACCAGCAA TATGGATGTT TTTGTTCAGC AGTATGCTGA
      2041 TACAGTGAAA TATCTGTCCG AGAAAAAATA ATAAGCATGC ACCATTCCTT GCGGCGGCGG
      2101 TGCTCAACGG CCTCAACCTA CTACTGGGCT GCTTCCTAAT GCAGGAGTCG CATAAGGGAG
      2161 AGCGTCGAGA TCCCGGACAC CATCGAATGG CGCAAAACCT TTCGCGGTAT GGCATGATAG
      2221 CGCCCGGAAG AGAGTCAATT CAGGGTGGTG AATGTGAAAC CAGTAACGTT ATACGATGTC
      2281 GCAGAGTATG CCGGTGTCTC TTATCAGACC GTTTCCCGCG TGGTGAACCA GGCCAGCCAC
      2341 GTTTCTGCGA AAACGCGGGA AAAAGTGGAA GCGGCGATGG CGGAGCTGAA TTACATTCCC
      2401 AACCGCGTGG CACAACAACT GGCGGGCAAA CAGTCGTTGC TGATTGGCGT TGCCACCTCC
      2461 AGTCTGGCCC TGCACGCGCC GTCGCAAATT GTCGCGGCGA TTAAATCTCG CGCCGATCAA
      2521 CTGGGTGCCA GCGTGGTGGT GTCGATGGTA GAACGAAGCG GCGTCGAAGC CTGTAAAGCG
      2581 GCGGTGCACA ATCTTCTCGC GCAACGCGTC AGTGGGCTGA TCATTAACTA TCCGCTGGAT
      2641 GACCAGGATG CCATTGCTGT GGAAGCTGCC TGCACTAATG TTCCGGCGTT ATTTCTTGAT
      2701 GTCTCTGACC AGACACCCAT CAACAGTATT ATTTTCTCCC ATGAAGACGG TACGCGACTG
      2761 GGCGTGGAGC ATCTGGTCGC ATTGGGTCAC CAGCAAATCG CGCTGTTAGC GGGCCCATTA
      2821 AGTTCTGTCT CGGCGCGTCT GCGTCTGGCT GGCTGGCATA AATATCTCAC TCGCAATCAA
      2881 ATTCAGCCGA TAGCGGAACG GGAAGGCGAC TGGAGTGCCA TGTCCGGTTT TCAACAAACC
      2941 ATGCAAATGC TGAATGAGGG CATCGTTCCC ACTGCGATGC TGGTTGCCAA CGATCAGATG
      3001 GCGCTGGGCG CAATGCGCGC CATTACCGAG TCCGGGCTGC GCGTTGGTGC GGATATCTCG
      3061 GTAGTGGGAT ACGACGATAC CGAAGACAGC TCATGTTATA TCCCGCCGTT AACCACCATC
      3121 AAACAGGATT TTCGCCTGCT GGGGCAAACC AGCGTGGACC GCTTGCTGCA ACTCTCTCAG
      3181 GGCCAGGCGG TGAAGGGCAA TCAGCTGTTG CCCGTCTCAC TGGTGAAAAG AAAAACCACC
      3241 CTGGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG
      3301 GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTAAGTTA
      3361 GCTCACTCAT TAGGCACCGG GATCTCGACC GATGCCCTTG AGAGCCTTCA ACCCAGTCAG
      3421 CTCCTTCCGG TGGGCGCGGG GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT
      3481 CATGCAACTC GTAGGACAGG TGCCGGCAGC GCTCTGGGTC ATTTTCGGCG AGGACCGCTT
      3541 TCGCTGGAGC GCGACGATGA TCGGCCTGTC GCTTGCGGTA TTCGGAATCT TGCACGCCCT
      3601 CGCTCAAGCC TTCGTCACTG GTCCCGCCAC CAAACGTTTC GGCGAGAAGC AGGCCATTAT
      3661 CGCCGGCATG GCGGCCCCAC GGGTGCGCAT GATCGTGCTC CTGTCGTTGA GGACCCGGCT
      3721 AGGCTGGCGG GGTTGCCTTA CTGGTTAGCA GAATGAATCA CCGATACGCG AGCGAACGTG
      3781 AAGCGACTGC TGCTGCAAAA CGTCTGCGAC CTGAGCAACA ACATGAATGG TCTTCGGTTT
      3841 CCGTGTTTCG TAAAGTCTGG AAACGCGGAA GTCAGCGCCC TGCACCATTA TGTTCCGGAT
      3901 CTGCATCGCA GGATGCTGCT GGCTACCCTG TGGAACACCT ACATCTGTAT TAACGAAGCG
      3961 CTGGCATTGA CCCTGAGTGA TTTTTCTCTG GTCCCGCCGC ATCCATACCG CCAGTTGTTT
      4021 ACCCTCACAA CGTTCCAGTA ACCGGGCATG TTCATCATCA GTAACCCGTA TCGTGAGCAT
      4081 CCTCTCTCGT TTCATCGGTA TCATTACCCC CATGAACAGA AATCCCCCTT ACACGGAGGC
      4141 ATCAGTGACC AAACAGGAAA AAACCGCCCT TAACATGGCC CGCTTTATCA GAAGCCAGAC
      4201 ATTAACGCTT CTGGAGAAAC TCAACGAGCT GGACGCGGAT GAACAGGCAG ACATCTGTGA
      4261 ATCGCTTCAC GACCACGCTG ATGAGCTTTA CCGCAGCTGC CTCGCGCGTT TCGGTGATGA
      4321 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA
      4381 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC
      4441 AGCCATGACC CAGTCACGTA GCGATAGCGG AGTGTATACT GGCTTAACTA TGCGGCATCA
      4501 GAGCAGATTG TACTGAGAGT GCACCATATA TGCGGTGTGA AATACCGCAC AGATGCGTAA
      4561 GGAGAAAATA CCGCATCAGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG
      4621 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG
      4681 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC
      4741 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
      4801 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
      4861 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
      4921 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
      4981 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
      5041 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
      5101 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
      5161 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA
      5221 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
      5281 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
      5341 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
      5401 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
      5461 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
      5521 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
      5581 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
      5641 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA
      5701 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
      5761 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC
      5821 GCAACGTTGT TGCCATTGCT GCAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
      5881 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA
      5941 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
      6001 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
      6061 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA
      6121 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
      6181 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
      6241 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA
      6301 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
      6361 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA AATTATTGAA GCATTTATCA
      6421 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG
      6481 GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTAAATTG TAAGCGTTAA TGTGAACCAT
      6541 CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG
      6601 GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA
      6661 AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA
      6721 CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTCCCATTCG CCA
//