FEATURES Location/Qualifiers misc_feature 204..235 /note="LIC Site" Cleavage-site 223..243 /note="TEV Site" promoter complement(1833..1845) /note="T7 Promoter" terminator complement(26..72) /note="T7 Terminator" CDS 2363..3322 /note="Lac I cds" CDS complement(5511..6371) /note="bla cds" rep_origin 4753..4753 /note="pBR322 origin of replication" Binding-site complement(1720..1755) /note="HN6 tag" CDS complement(259..1716) /note="NusA" 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT 121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT 181 CGACGGAGCT CGAATTCGGA TCCGTTATCC ACTTCCAATA TTGGATTGGA AGTACAGGTT 241 CTCGTTCTCG TTGTTGTTAT TACGGGCAGC CATAATCAGT GCTCCGGCTT TTTCGTCGGT 301 CAACCCTTCG ATATCAGCCA GATCATCAAT GCCCTGTTCG GCGAGATCTT CCAGCGTACA 361 AACGCCACGG GCGGCCAGTT TGAATGCCAA ATCACGATCT ACCCCTTCAA GGTTCAGCAG 421 ATCGTCAGCC GGTTTGTTAT CACCGAGGCT TTCTTCCTGG GCCTGTGCAA TGGTGGCCAG 481 TGCATTTTTA GCACGCTCGC GCAGTGCTTC AACGGTCGGC TCATCAAGGC CTTCGATTTC 541 CAACAGCTCT TTCATCGGCA CATAGGCCAA TTCTTCCAGC GTCGAGAAGC CTTCTTCTAC 601 CAGAACAGTC GCGAAGTCTT CGTCGATGTC GAGATATTTG GTGAAGGTGT CGATCGCTGC 661 GTGCGCTTCC GCCTGATGCT TAGCTTGCAG GTCGTCAACG GTCATCACGT TGAGTTCCCA 721 ACCGCTCAGT TGCGAAGCCA GACGCACGTT CTGACCGTTA CGGCCAATCG CCTGCGCCAG 781 ATTACCGGCT TCAACGGCGA TGTCCATGGT GTGTTTATCT TCATCCACCA CGATAGAAGC 841 AACGTCTGCC GGTGCCATTG CGTTAATCAC GAACTGCGCC GGGTTATCAT CCCACAGGAC 901 GATATCGATA CGCTCGCCAC CCAGTTCAGT AGACACCGCC TGAACACGCG CGCCACGCAT 961 ACCTACGCAA GCACCTACCG GATCGATACG TTTATCGTTG GTTTTCACCG CGATTTTCGC 1021 ACGAGAACCC GGATCGCGAG CCGCTGCTTT AATTTCAATC ACTTCTTCGC CGATTTCTGG 1081 CACTTCAATA CGGAACAGTT CGATCAGCAT TTCCGGCTTG GAACGAGTGA CGAACAGTTG 1141 CGCGCCACGC GCTTCCGGGC GAACGGAATA GAGCACGCCA CGAACGCGGT CGCCAGGGCG 1201 GAAGTTTTCA CGCGGCAGCA TATCTTCGCG CAGGATCACG GCTTCAGCGT TGTTGCCCAG 1261 ATCCAGAGAG ATGTTGTCGC GGTTTACTTT TTTCACCACG CCGGTGATGA TTTCACCTTC 1321 GTGTTCACGG AACTGATCAA CCACCATCGC ACGTTCGGCT TCACGCACTT TCTGCACGAT 1381 AACCTGTTTT GCCGTCTGGG TAGTGATACG GTCAAAGGTA ACAGACTCAA TCTGATCTTC 1441 AACGTAATCG CCCAGGTTCA GGCTTTCATC TTCATAACGT GCGGCTTCAA GGGTGATTTC 1501 CTTGGTCGGC TGGGTGACTT CATCAACAAC TAACCAGCGA CGGAAAGTGT CAAAATCACC 1561 GCTTTTGCGA TCGATCTGTA CGCGGACGTC GATCTCTTGT TCATATTTTT TCTTTGTTGC 1621 TGTCGCCAGC GCGCTTTCCA ATGCTTCGAA AATCTTCTCG CGAGGTAGCG CCTTTTCATT 1681 GGATACGGCT TCAACTACAG CCAAAATTTC TTTGTTGTTG TGATTATGGT TGTGGTTGTG 1741 GTTATGATTA TGGTTCGGCA TATGTATATC TCCTTCTTAA AGTTAAACAA AATTATTTCT 1801 AGAGGGGAAT TGTTATCCGC TCACAATTCC CCTATAGTGA GTCGTATTAA TTTCGCGGGA 1861 TCGAGATCGA TCTCGATCCT CTACGCCGGA CGCATCGTGG CCGGCATCAC CGGCGCCACA 1921 GGTGCGGTTG CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG GGCTCGCCAC 1981 TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT GGCCGGGGGA 2041 CTGTTGGGCG CCATCTCCTT GCATGCACCA TTCCTTGCGG CGGCGGTGCT CAACGGCCTC 2101 AACCTACTAC TGGGCTGCTT CCTAATGCAG GAGTCGCATA AGGGAGAGCG TCGAGATCCC 2161 GGACACCATC GAATGGCGCA AAACCTTTCG CGGTATGGCA TGATAGCGCC CGGAAGAGAG 2221 TCAATTCAGG GTGGTGAATG TGAAACCAGT AACGTTATAC GATGTCGCAG AGTATGCCGG 2281 TGTCTCTTAT CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT CTGCGAAAAC 2341 GCGGGAAAAA GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC GCGTGGCACA 2401 ACAACTGGCG GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA 2461 CGCGCCGTCG CAAATTGTCG CGGCGATTAA ATCTCGCGCC GATCAACTGG GTGCCAGCGT 2521 GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT CGAAGCCTGT AAAGCGGCGG TGCACAATCT 2581 TCTCGCGCAA CGCGTCAGTG GGCTGATCAT TAACTATCCG CTGGATGACC AGGATGCCAT 2641 TGCTGTGGAA GCTGCCTGCA CTAATGTTCC GGCGTTATTT CTTGATGTCT CTGACCAGAC 2701 ACCCATCAAC AGTATTATTT TCTCCCATGA AGACGGTACG CGACTGGGCG TGGAGCATCT 2761 GGTCGCATTG GGTCACCAGC AAATCGCGCT GTTAGCGGGC CCATTAAGTT CTGTCTCGGC 2821 GCGTCTGCGT CTGGCTGGCT GGCATAAATA TCTCACTCGC AATCAAATTC AGCCGATAGC 2881 GGAACGGGAA GGCGACTGGA GTGCCATGTC CGGTTTTCAA CAAACCATGC AAATGCTGAA 2941 TGAGGGCATC GTTCCCACTG CGATGCTGGT TGCCAACGAT CAGATGGCGC TGGGCGCAAT 3001 GCGCGCCATT ACCGAGTCCG GGCTGCGCGT TGGTGCGGAT ATCTCGGTAG TGGGATACGA 3061 CGATACCGAA GACAGCTCAT GTTATATCCC GCCGTTAACC ACCATCAAAC AGGATTTTCG 3121 CCTGCTGGGG CAAACCAGCG TGGACCGCTT GCTGCAACTC TCTCAGGGCC AGGCGGTGAA 3181 GGGCAATCAG CTGTTGCCCG TCTCACTGGT GAAAAGAAAA ACCACCCTGG CGCCCAATAC 3241 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC 3301 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT AAGTTAGCTC ACTCATTAGG 3361 CACCGGGATC TCGACCGATG CCCTTGAGAG CCTTCAACCC AGTCAGCTCC TTCCGGTGGG 3421 CGCGGGGCAT GACTATCGTC GCCGCACTTA TGACTGTCTT CTTTATCATG CAACTCGTAG 3481 GACAGGTGCC GGCAGCGCTC TGGGTCATTT TCGGCGAGGA CCGCTTTCGC TGGAGCGCGA 3541 CGATGATCGG CCTGTCGCTT GCGGTATTCG GAATCTTGCA CGCCCTCGCT CAAGCCTTCG 3601 TCACTGGTCC CGCCACCAAA CGTTTCGGCG AGAAGCAGGC CATTATCGCC GGCATGGCGG 3661 CCCCACGGGT GCGCATGATC GTGCTCCTGT CGTTGAGGAC CCGGCTAGGC TGGCGGGGTT 3721 GCCTTACTGG TTAGCAGAAT GAATCACCGA TACGCGAGCG AACGTGAAGC GACTGCTGCT 3781 GCAAAACGTC TGCGACCTGA GCAACAACAT GAATGGTCTT CGGTTTCCGT GTTTCGTAAA 3841 GTCTGGAAAC GCGGAAGTCA GCGCCCTGCA CCATTATGTT CCGGATCTGC ATCGCAGGAT 3901 GCTGCTGGCT ACCCTGTGGA ACACCTACAT CTGTATTAAC GAAGCGCTGG CATTGACCCT 3961 GAGTGATTTT TCTCTGGTCC CGCCGCATCC ATACCGCCAG TTGTTTACCC TCACAACGTT 4021 CCAGTAACCG GGCATGTTCA TCATCAGTAA CCCGTATCGT GAGCATCCTC TCTCGTTTCA 4081 TCGGTATCAT TACCCCCATG AACAGAAATC CCCCTTACAC GGAGGCATCA GTGACCAAAC 4141 AGGAAAAAAC CGCCCTTAAC ATGGCCCGCT TTATCAGAAG CCAGACATTA ACGCTTCTGG 4201 AGAAACTCAA CGAGCTGGAC GCGGATGAAC AGGCAGACAT CTGTGAATCG CTTCACGACC 4261 ACGCTGATGA GCTTTACCGC AGCTGCCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT 4321 GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC 4381 AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC ATGACCCAGT 4441 CACGTAGCGA TAGCGGAGTG TATACTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT 4501 GAGAGTGCAC CATATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 4561 ATCAGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG 4621 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC 4681 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG 4741 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA 4801 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 4861 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC 4921 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG 4981 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC 5041 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA 5101 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 5161 AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG 5221 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 5281 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA 5341 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 5401 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA 5461 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC 5521 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA 5581 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA 5641 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC 5701 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT 5761 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC 5821 ATTGCTGCAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT 5881 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC 5941 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG 6001 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT 6061 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG 6121 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA 6181 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG 6241 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG 6301 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT 6361 TGAATACTCA TACTCTTCCT TTTTCAAATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 6421 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 6481 TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATGTG AACCATCACC CTAATCAAGT 6541 TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT 6601 AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA 6661 GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC 6721 GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCA //