FEATURES Location/Qualifiers promoter complement(1815..1827) /note="T7 Promoter" CDS 2345..3304 /note="Lac I cds" CDS complement(5493..6353) /note="bla cds" rep_origin 4735..4735 /note="pBR322 origin of replication" terminator complement(26..72) /note="T7 Terminator" CDS complement(252..1710) /note="NusA cds" Cleavage-site complement(223..243) /note="TEV site" misc_feature complement(205..234) /note="LIC site" misc_feature complement(1717..1740) /note="His8" 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT 121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT 181 CGACGGAGCT CGAATTCGGA TCCGTTATCC ACTTCCAATA TTGGATTGGA AGTACAGGTT 241 CTCGTTGTTG TTATTACGGG CAGCCATAAT CAGTGCTCCG GCTTTTTCGT CGGTCAACCC 301 TTCGATATCA GCCAGATCAT CAATGCCCTG TTCGGCGAGA TCTTCCAGCG TACAAACGCC 361 ACGGGCGGCC AGTTTGAATG CCAAATCACG ATCTACCCCT TCAAGGTTCA GCAGATCGTC 421 AGCCGGTTTG TTATCACCGA GGCTTTCTTC CTGGGCCTGT GCAATGGTGG CCAGTGCATT 481 TTTAGCACGC TCGCGCAGTG CTTCAACGGT CGGCTCATCA AGGCCTTCGA TTTCCAACAG 541 CTCTTTCATC GGCACATAGG CCAATTCTTC CAGCGTCGAG AAGCCTTCTT CTACCAGAAC 601 AGTCGCGAAG TCTTCGTCGA TGTCGAGATA TTTGGTGAAG GTGTCGATCG CTGCGTGCGC 661 TTCCGCCTGA TGCTTAGCTT GCAGGTCGTC AACGGTCATC ACGTTGAGTT CCCAACCGCT 721 CAGTTGCGAA GCCAGACGCA CGTTCTGACC GTTACGGCCA ATCGCCTGCG CCAGATTACC 781 GGCTTCAACG GCGATGTCCA TGGTGTGTTT ATCTTCATCC ACCACGATAG AAGCAACGTC 841 TGCCGGTGCC ATTGCGTTAA TCACGAACTG CGCCGGGTTA TCATCCCACA GGACGATATC 901 GATACGCTCG CCACCCAGTT CAGTAGACAC CGCCTGAACA CGCGCGCCAC GCATACCTAC 961 GCAAGCACCT ACCGGATCGA TACGTTTATC GTTGGTTTTC ACCGCGATTT TCGCACGAGA 1021 ACCCGGATCG CGAGCCGCTG CTTTAATTTC AATCACTTCT TCGCCGATTT CTGGCACTTC 1081 AATACGGAAC AGTTCGATCA GCATTTCCGG CTTGGAACGA GTGACGAACA GTTGCGCGCC 1141 ACGCGCTTCC GGGCGAACGG AATAGAGCAC GCCACGAACG CGGTCGCCAG GGCGGAAGTT 1201 TTCACGCGGC AGCATATCTT CGCGCAGGAT CACGGCTTCA GCGTTGTTGC CCAGATCCAG 1261 AGAGATGTTG TCGCGGTTTA CTTTTTTCAC CACGCCGGTG ATGATTTCAC CTTCGTGTTC 1321 ACGGAACTGA TCAACCACCA TCGCACGTTC GGCTTCACGC ACTTTCTGCA CGATAACCTG 1381 TTTTGCCGTC TGGGTAGTGA TACGGTCAAA GGTAACAGAC TCAATCTGAT CTTCAACGTA 1441 ATCGCCCAGG TTCAGGCTTT CATCTTCATA ACGTGCGGCT TCAAGGGTGA TTTCCTTGGT 1501 CGGCTGGGTG ACTTCATCAA CAACTAACCA GCGACGGAAA GTGTCAAAAT CACCGCTTTT 1561 GCGATCGATC TGTACGCGGA CGTCGATCTC TTGTTCATAT TTTTTCTTTG TTGCTGTCGC 1621 CAGCGCGCTT TCCAATGCTT CGAAAATCTT CTCGCGAGGT AGCGCCTTTT CATTGGATAC 1681 GGCTTCAACT ACAGCCAAAA TTTCTTTGTT AGAAGAATGA TGATGATGAT GATGATGGTG 1741 CATATGTATA TCTCCTTCTT AAAGTTAAAC AAAATTATTT CTAGAGGGGA ATTGTTATCC 1801 GCTCACAATT CCCCTATAGT GAGTCGTATT AATTTCGCGG GATCGAGATC GATCTCGATC 1861 CTCTACGCCG GACGCATCGT GGCCGGCATC ACCGGCGCCA CAGGTGCGGT TGCTGGCGCC 1921 TATATCGCCG ACATCACCGA TGGGGAAGAT CGGGCTCGCC ACTTCGGGCT CATGAGCGCT 1981 TGTTTCGGCG TGGGTATGGT GGCAGGCCCC GTGGCCGGGG GACTGTTGGG CGCCATCTCC 2041 TTGCATGCAC CATTCCTTGC GGCGGCGGTG CTCAACGGCC TCAACCTACT ACTGGGCTGC 2101 TTCCTAATGC AGGAGTCGCA TAAGGGAGAG CGTCGAGATC CCGGACACCA TCGAATGGCG 2161 CAAAACCTTT CGCGGTATGG CATGATAGCG CCCGGAAGAG AGTCAATTCA GGGTGGTGAA 2221 TGTGAAACCA GTAACGTTAT ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT 2281 TTCCCGCGTG GTGAACCAGG CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC 2341 GGCGATGGCG GAGCTGAATT ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA 2401 GTCGTTGCTG ATTGGCGTTG CCACCTCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT 2461 CGCGGCGATT AAATCTCGCG CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA 2521 ACGAAGCGGC GTCGAAGCCT GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG 2581 TGGGCTGATC ATTAACTATC CGCTGGATGA CCAGGATGCC ATTGCTGTGG AAGCTGCCTG 2641 CACTAATGTT CCGGCGTTAT TTCTTGATGT CTCTGACCAG ACACCCATCA ACAGTATTAT 2701 TTTCTCCCAT GAAGACGGTA CGCGACTGGG CGTGGAGCAT CTGGTCGCAT TGGGTCACCA 2761 GCAAATCGCG CTGTTAGCGG GCCCATTAAG TTCTGTCTCG GCGCGTCTGC GTCTGGCTGG 2821 CTGGCATAAA TATCTCACTC GCAATCAAAT TCAGCCGATA GCGGAACGGG AAGGCGACTG 2881 GAGTGCCATG TCCGGTTTTC AACAAACCAT GCAAATGCTG AATGAGGGCA TCGTTCCCAC 2941 TGCGATGCTG GTTGCCAACG ATCAGATGGC GCTGGGCGCA ATGCGCGCCA TTACCGAGTC 3001 CGGGCTGCGC GTTGGTGCGG ATATCTCGGT AGTGGGATAC GACGATACCG AAGACAGCTC 3061 ATGTTATATC CCGCCGTTAA CCACCATCAA ACAGGATTTT CGCCTGCTGG GGCAAACCAG 3121 CGTGGACCGC TTGCTGCAAC TCTCTCAGGG CCAGGCGGTG AAGGGCAATC AGCTGTTGCC 3181 CGTCTCACTG GTGAAAAGAA AAACCACCCT GGCGCCCAAT ACGCAAACCG CCTCTCCCCG 3241 CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA 3301 GTGAGCGCAA CGCAATTAAT GTAAGTTAGC TCACTCATTA GGCACCGGGA TCTCGACCGA 3361 TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC ATGACTATCG 3421 TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG CCGGCAGCGC 3481 TCTGGGTCAT TTTCGGCGAG GACCGCTTTC GCTGGAGCGC GACGATGATC GGCCTGTCGC 3541 TTGCGGTATT CGGAATCTTG CACGCCCTCG CTCAAGCCTT CGTCACTGGT CCCGCCACCA 3601 AACGTTTCGG CGAGAAGCAG GCCATTATCG CCGGCATGGC GGCCCCACGG GTGCGCATGA 3661 TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG GCTGGCGGGG TTGCCTTACT GGTTAGCAGA 3721 ATGAATCACC GATACGCGAG CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT 3781 GAGCAACAAC ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT 3841 CAGCGCCCTG CACCATTATG TTCCGGATCT GCATCGCAGG ATGCTGCTGG CTACCCTGTG 3901 GAACACCTAC ATCTGTATTA ACGAAGCGCT GGCATTGACC CTGAGTGATT TTTCTCTGGT 3961 CCCGCCGCAT CCATACCGCC AGTTGTTTAC CCTCACAACG TTCCAGTAAC CGGGCATGTT 4021 CATCATCAGT AACCCGTATC GTGAGCATCC TCTCTCGTTT CATCGGTATC ATTACCCCCA 4081 TGAACAGAAA TCCCCCTTAC ACGGAGGCAT CAGTGACCAA ACAGGAAAAA ACCGCCCTTA 4141 ACATGGCCCG CTTTATCAGA AGCCAGACAT TAACGCTTCT GGAGAAACTC AACGAGCTGG 4201 ACGCGGATGA ACAGGCAGAC ATCTGTGAAT CGCTTCACGA CCACGCTGAT GAGCTTTACC 4261 GCAGCTGCCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG 4321 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT 4381 CAGCGGGTGT TGGCGGGTGT CGGGGCGCAG CCATGACCCA GTCACGTAGC GATAGCGGAG 4441 TGTATACTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATATG 4501 CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CTCTTCCGCT 4561 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC 4621 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 4681 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 4741 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 4801 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 4861 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 4921 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 4981 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 5041 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 5101 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 5161 GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 5221 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 5281 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT 5341 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 5401 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 5461 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT 5521 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA 5581 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA 5641 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA 5701 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA 5761 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTGC AGGCATCGTG 5821 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 5881 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 5941 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 6001 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 6061 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT 6121 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 6181 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 6241 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 6301 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 6361 CTTTTTCAAA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 6421 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 6481 CTAAATTGTA AGCGTTAATG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC 6541 CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG 6601 CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG 6661 GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA 6721 CAGGGCGCGT CCCATTCGCC A //