COMMENT pMCSG58 4748 bp base vector: pMCSG53 LIC region plus a portion of the beta-lactamase gene from pMCSG26 removed by digestion with BglI and XbaI then ligated into pMCSG53 digested with the same enzymes. Product is C-terminal His-tag plasmid with truncated ori and the argU & ileX tRNA genes FEATURES Location/Qualifiers tRNA complement(899..1050) /note="ileX gene" rep_origin complement(2741..3329) /note="pBR322 origin of replication" CDS 1353..2312 /note="Lac I cds" CDS complement(3500..4360) /note="bla cds" tRNA complement(383..739) /note="argU tRNA" misc_feature complement(217..238) /note="LIC Site" Region complement(208..225) /note="His Tag" promoter complement(300..316) /note="T7 Promoter" terminator complement(26..72) /note="T7 Terminator" 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT 121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT 181 CGACGGAGCT CGAATTCGGA TCCGTTAATG ATGATGATGG TGGTGCCCGG GAGAGACTCC 241 TTCTTAAAGT TAAACAAAAT TATTTCTAGA GGGGAATTGT TATCCGCTCA CAATTCCCCT 301 ATAGTGAGTC GTATTAATTT CGCGGGATCG AGATCGATCT CGATCCTCTA CGCCGGACGC 361 ATCGTGGCCG GCATCACCGG TGGATTCAGT CAGGCGTCCC ATTATCAGTG CTTCAGGAAA 421 TGGGCGGATG GGAGTCCATA GAAATGGTTC GTAGGTATGC TCACCTTGCG CCTAATCATT 481 TGACAGAGCA TGCGAGGAAA ATAGACGACA TTTTTGGTGA TAATGTCCCA AATATGTCCC 541 ACTCTGGAAT TATGGAGGAT ATAAAGAAGG CGTAACTGAT TGAATTGTAA TGGCGCGCCC 601 TGCAGGATTC GAACCTGCGG CCCACGACTT AGAAGGTCGT TGCTCTATCC AACTGAGCTA 661 AGGGCGCGTT GATACCGCAA TGCGGTGTAA TCGCGTGAAT TATACGGTCA ACCCTTGCTG 721 AGTCAATGGC TTTTGATCTC GCCGGCGCCA CAGGTGCGGT TGCTGGCGCC TATATCGCCG 781 ACATCACCGA TGGGGAAGAT CGGGCTCGCC ACTTCGGGCT CATGAGCGCT TGTTTCGGCG 841 TGGGTATGGT GGCAGGCCCC GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTGCATGCAA 901 AAAGGCTGAC GATTTCTCGT CAGCCTTTGC TATATCTGGT GGCCCCTGCT GGACTTGAAC 961 CAGCGACCAA GCGATTATGA GTCGCCTGCT CTAACCACTG AGCTAAGGGG CCATGGTAGC 1021 GGATTATAAA GTAACTCCGT GTCGCAATCC GCATGCACCA TTCCTTGCGG CGGCGGTGCT 1081 CAACGGCCTC AACCTACTAC TGGGCTGCTT CCTAATGCAG GAGTCGCATA AGGGAGAGCG 1141 TCGAGATCCC GGACACCATC GAATGGCGCA AAACCTTTCG CGGTATGGCA TGATAGCGCC 1201 CGGAAGAGAG TCAATTCAGG GTGGTGAATG TGAAACCAGT AACGTTATAC GATGTCGCAG 1261 AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT 1321 CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC 1381 GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC 1441 TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA ATCTCGCGCC GATCAACTGG 1501 GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT CGAAGCCTGT AAAGCGGCGG 1561 TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT TAACTATCCG CTGGATGACC 1621 AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC GGCGTTATTT CTTGATGTCT 1681 CTGACCAGAC ACCCATCAAC AGTATTATTT TCTCCCATGA AGACGGTACG CGACTGGGCG 1741 TGGAGCATCT GGTCGCATTG GGTCACCAGC AAATCGCGCT GTTAGCGGGC CCATTAAGTT 1801 CTGTCTCGGC GCGTCTGCGT CTGGCTGGCT GGCATAAATA TCTCACTCGC AATCAAATTC 1861 AGCCGATAGC GGAACGGGAA GGCGACTGGA GTGCCATGTC CGGTTTTCAA CAAACCATGC 1921 AAATGCTGAA TGAGGGCATC GTTCCCACTG CGATGCTGGT TGCCAACGAT CAGATGGCGC 1981 TGGGCGCAAT GCGCGCCATT ACCGAGTCCG GGCTGCGCGT TGGTGCGGAT ATCTCGGTAG 2041 TGGGATACGA CGATACCGAA GACAGCTCAT GTTATATCCC GCCGTTAACC ACCATCAAAC 2101 AGGATTTTCG CCTGCTGGGG CAAACCAGCG TGGACCGCTT GCTGCAACTC TCTCAGGGCC 2161 AGGCGGTGAA GGGCAATCAG CTGTTGCCCG TCTCACTGGT GAAAAGAAAA ACCACCCTGG 2221 CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC 2281 GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT AAGTTAGCTC 2341 ACTCATTAGG CACCGGGATC TCGACCGATG CCCTTGAGAG CCTTCAACCC AGTCAGCTCC 2401 TTCCGGTGGG CGCGGGGCAT GACTACAGTC ACGTAGCGAT AGCGGAGTGT ATACTGGCTT 2461 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATATGCGG TGTGAAATAC 2521 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCTC TTCCGCTTCC TCGCTCACTG 2581 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 2641 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 2701 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 2761 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 2821 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 2881 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 2941 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 3001 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 3061 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 3121 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 3181 GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 3241 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC 3301 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 3361 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 3421 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 3481 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 3541 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 3601 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 3661 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 3721 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 3781 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTGCAGG CATCGTGGTG TCACGCTCGT 3841 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 3901 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 3961 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 4021 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 4081 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 4141 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 4201 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 4261 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 4321 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAAATTA 4381 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 4441 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTA AATTGTAAGC 4501 GTTAATGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA 4561 ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG 4621 CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG 4681 TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCCC 4741 ATTCGCCA //