COMMENT pMCSG61 6422 bp Base Vector: pMCSG30 SacB cds removed from MBP fusion vector pMCSG30. LIC site modified (no restriction site) for PCR propagation. MBP sequence modified for better crystallization. FEATURES Location/Qualifiers Cleavage-site complement(1359..1379) /note="TEV Site" Region complement(1404..1421) /note="His Tag" promoter complement(1496..1512) /note="T7 Promoter" terminator complement(26..72) /note="T7 Terminator" CDS 2026..2985 /note="Lac I cds" CDS complement(5174..6034) /note="bla cds" rep_origin 4416..4416 /note="pBR322 origin of replication" misc_feature complement(279..1355) /note="MBP" primer_bind complement(203..243) /note="LIC-F" primer_bind complement(244..271) /note="LIC-R" 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCACCTTCT TTTCGGGCTT 121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT 181 CGACGGAGCT CGAATTCGGA TCCGTTATCC ACTTCCAATG TTATTTTTCA CCGACGACAA 241 GGATGCTGCT GCGTTGGTTT GAGCAGCGGC AAGAGCAGCA TCGACAGTCT GACGACCGCT 301 GGCGGCGTTG ATCACCGCAG TACGCACGGC ATACCAGAAA GCGGACATCT GCGGGATGTT 361 CGGCATGATT TCACCTTTCT GGGCGTTTTC CATGGTGGCG GCAATACGTG GATCTTTCGC 421 CAACTCTTCC TCGTAAGACT TCAGCGCTAC GGCACCCAGC GGTTTGTCTT TATTAACCGC 481 TTCCAGACCT TCATCAGTCA GCAGATAGTT TTCGAGGAAC TCTTTCGCCA GCTCTTTGTT 541 CGGACTGGCG GCGTTAATAC CTGCGCTCAG CACGCCAACG AACGGTTTGG ATGGTTGACC 601 CTTGAAGGTC GGCAGTACCG TTACACCATA ATTCACTGCG CTGGTGTCGA TGTTGGACCA 661 TGCCCACGGG CCGTTGATGG TCATCGCTGT TTCGCCTTTA TTAAAGGCAG CTTCTGCGAT 721 GGAGTAATCG GTGTCTGCAT TCATGTGTTT GTTTTTAATC AGGTCAACCA GGAAGGTCAG 781 ACCCGCTTTC GCGCCAGCGT TATCCACGCC CACGTCTTTA ATGTCGTACT TGCCTGCTGC 841 ATACTTGAAC GCATAACCCC CGTCAGCAGC AATCAGCGGC CAGGTGAAGT ACGGTTCTTG 901 CAGGTTGAAC ATCAGCGCGC TCTTACCTTT CGCTTTCAGT TCTTTATCCA GCGCCGGGAT 961 CTCTTCCCAG GTTTTTGGCG GGTTCGGCAG CAGATCTTTG TTATAAATCA GCGATAACGC 1021 TTCAACAGCG ATCGGGTAAG CAATCAGCTT GCCGTTGTAA CGTACGGCAT CCCAGGTAAA 1081 CGGATACAGC TTGTCCTGGA ACGCTGCTGC CGGGGTGATT TCAGCCAACA GGCCAGATTG 1141 AGCGTAGCCA CCAAAGCGGT CGTGTGCCCA GAAGATAATG TCAGGGCCAT CGCCAGTTGC 1201 CGCAACCTGT GGGAATTTCT CTTCCAGTTT ATCCGGATGC TCAACGGTGA CTTTAATTCC 1261 GGTATCTTTC TCGAATTTCT TACCGACTTC AGCGAGACCG TTATAGCCTT TATCGCCGTT 1321 AATCCAGATT ACCAGTTTAC CTTCTTCGAT TTTGGCATTG GATTGGAAGT ACAGGTTCTC 1381 GGTACCCAGA TCTACACCAG AAGAATGATG ATGATGATGG TGCATATGTA TATCTCCTTC 1441 TTAAAGTTAA ACAAAATTAT TCTAGAGGGG AATTGTTATC CGCTCACAAT TCCCCTATAG 1501 TGAGTCGTAT TAATTTCGCG GGATCGAGAT CGATCTCGAT CCTCTACGCC GGACGCATCG 1561 TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC GACATCACCG 1621 ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC GTGGGTATGG 1681 TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA CCATTCCTTG 1741 CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC 1801 ATAAGGGAGA GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG 1861 GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC AGTAACGTTA 1921 TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG 1981 GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT 2041 TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT 2101 GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC 2161 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC 2221 TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT 2281 CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA 2341 TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT 2401 ACGCGACTGG GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG 2461 GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA ATATCTCACT 2521 CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT GTCCGGTTTT 2581 CAACAAACCA TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT GGTTGCCAAC 2641 GATCAGATGG CGCTGGGCGC AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG 2701 GATATCTCGG TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA 2761 ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG CTTGCTGCAA 2821 CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC CCGTCTCACT GGTGAAAAGA 2881 AAAACCACCC TGGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA 2941 ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA 3001 TGTAAGTTAG CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA GAGCCTTCAA 3061 CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT 3121 CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTGGGTCA TTTTCGGCGA 3181 GGACCGCTTT CGCTGGAGCG CGACGATGAT CGGCCTGTCG CTTGCGGTAT TCGGAATCTT 3241 GCACGCCCTC GCTCAAGCCT TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA 3301 GGCCATTATC GCCGGCATGG CGGCCCCACG GGTGCGCATG ATCGTGCTCC TGTCGTTGAG 3361 GACCCGGCTA GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC CGATACGCGA 3421 GCGAACGTGA AGCGACTGCT GCTGCAAAAC GTCTGCGACC TGAGCAACAA CATGAATGGT 3481 CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA AACGCGGAAG TCAGCGCCCT GCACCATTAT 3541 GTTCCGGATC TGCATCGCAG GATGCTGCTG GCTACCCTGT GGAACACCTA CATCTGTATT 3601 AACGAAGCGC TGGCATTGAC CCTGAGTGAT TTTTCTCTGG TCCCGCCGCA TCCATACCGC 3661 CAGTTGTTTA CCCTCACAAC GTTCCAGTAA CCGGGCATGT TCATCATCAG TAACCCGTAT 3721 CGTGAGCATC CTCTCTCGTT TCATCGGTAT CATTACCCCC ATGAACAGAA ATCCCCCTTA 3781 CACGGAGGCA TCAGTGACCA AACAGGAAAA AACCGCCCTT AACATGGCCC GCTTTATCAG 3841 AAGCCAGACA TTAACGCTTC TGGAGAAACT CAACGAGCTG GACGCGGATG AACAGGCAGA 3901 CATCTGTGAA TCGCTTCACG ACCACGCTGA TGAGCTTTAC CGCAGCTGCC TCGCGCGTTT 3961 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT 4021 GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG 4081 TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA GTGTATACTG GCTTAACTAT 4141 GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATAT GCGGTGTGAA ATACCGCACA 4201 GATGCGTAAG GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 4261 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 4321 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 4381 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 4441 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 4501 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 4561 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 4621 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 4681 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 4741 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 4801 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG 4861 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 4921 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA 4981 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 5041 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 5101 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 5161 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 5221 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 5281 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 5341 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 5401 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 5461 ATAGTTTGCG CAACGTTGTT GCCATTGCTG CAGGCATCGT GGTGTCACGC TCGTCGTTTG 5521 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 5581 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 5641 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 5701 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 5761 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 5821 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 5881 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 5941 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 6001 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA ATTATTGAAG 6061 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 6121 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTAAATTGT AAGCGTTAAT 6181 GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA 6241 ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA 6301 AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC 6361 TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCCCATTCGC 6421 CA //