COMMENT
     pMCSG63         4864 bp
     base vector:    pMCSG53
     LIC regions plus a portion of the beta-lactamase gene from pMCSG60 removed
     by digestion with BglI and XbaI then ligated into pMCSG53 digested with   
     the same enzymes. Product is dual-LIC plasmid with truncated ori     
     and the argU & ileX tRNA genes
FEATURES             Location/Qualifiers
     tRNA            complement(1015..1166)
                     /note="ileX gene"
     rep_origin      complement(2857..3445)
                     /note="pBR322 origin of replication"
     CDS             1469..2428
                     /note="Lac I cds"
     CDS             complement(3616..4476)
                     /note="bla cds"
     tRNA            complement(499..855)
                     /note="argU tRNA"
     misc_feature    complement(260..291)
                     /note="MCSG7 LIC site"
     Cleavage-site   complement(279..299)
                     /note="TEV Site"
     Region          complement(324..341)
                     /note="His Tag"
     terminator      complement(26..72)
                     /note="T7 Terminator"
     misc_feature    complement(223..244)
                     /note="MCSG26 LIC site"
     RBS             complement(243..248)
                     /note="ribosome binding site"
     RBS             complement(353..358)
                     /note="ribosome binding site"
     promoter        complement(416..432)
                     /note="T7 Promoter"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTATCC ACTTCCAATG TTATGGTGGT GCCCGGGAGA
       241 GACTCCTTCT TAAAGTTAAG TTATCCACTT CCAATATTGG ATTGGAAGTA CAGGTTCTCG
       301 GTACCCAGAT CTACACCAGA AGAATGATGA TGATGATGGT GCATATGTAT ATCTCCTTCT
       361 TAAAGTTAAA CAAAATTATT TCTAGAGGGG AATTGTTATC CGCTCACAAT TCCCCTATAG
       421 TGAGTCGTAT TAATTTCGCG GGATCGAGAT CGATCTCGAT CCTCTACGCC GGACGCATCG
       481 TGGCCGGCAT CACCGGTGGA TTCAGTCAGG CGTCCCATTA TCAGTGCTTC AGGAAATGGG
       541 CGGATGGGAG TCCATAGAAA TGGTTCGTAG GTATGCTCAC CTTGCGCCTA ATCATTTGAC
       601 AGAGCATGCG AGGAAAATAG ACGACATTTT TGGTGATAAT GTCCCAAATA TGTCCCACTC
       661 TGGAATTATG GAGGATATAA AGAAGGCGTA ACTGATTGAA TTGTAATGGC GCGCCCTGCA
       721 GGATTCGAAC CTGCGGCCCA CGACTTAGAA GGTCGTTGCT CTATCCAACT GAGCTAAGGG
       781 CGCGTTGATA CCGCAATGCG GTGTAATCGC GTGAATTATA CGGTCAACCC TTGCTGAGTC
       841 AATGGCTTTT GATCTCGCCG GCGCCACAGG TGCGGTTGCT GGCGCCTATA TCGCCGACAT
       901 CACCGATGGG GAAGATCGGG CTCGCCACTT CGGGCTCATG AGCGCTTGTT TCGGCGTGGG
       961 TATGGTGGCA GGCCCCGTGG CCGGGGGACT GTTGGGCGCC ATCTCCTTGC ATGCAAAAAG
      1021 GCTGACGATT TCTCGTCAGC CTTTGCTATA TCTGGTGGCC CCTGCTGGAC TTGAACCAGC
      1081 GACCAAGCGA TTATGAGTCG CCTGCTCTAA CCACTGAGCT AAGGGGCCAT GGTAGCGGAT
      1141 TATAAAGTAA CTCCGTGTCG CAATCCGCAT GCACCATTCC TTGCGGCGGC GGTGCTCAAC
      1201 GGCCTCAACC TACTACTGGG CTGCTTCCTA ATGCAGGAGT CGCATAAGGG AGAGCGTCGA
      1261 GATCCCGGAC ACCATCGAAT GGCGCAAAAC CTTTCGCGGT ATGGCATGAT AGCGCCCGGA
      1321 AGAGAGTCAA TTCAGGGTGG TGAATGTGAA ACCAGTAACG TTATACGATG TCGCAGAGTA
      1381 TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC CAGGCCAGCC ACGTTTCTGC
      1441 GAAAACGCGG GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG AATTACATTC CCAACCGCGT
      1501 GGCACAACAA CTGGCGGGCA AACAGTCGTT GCTGATTGGC GTTGCCACCT CCAGTCTGGC
      1561 CCTGCACGCG CCGTCGCAAA TTGTCGCGGC GATTAAATCT CGCGCCGATC AACTGGGTGC
      1621 CAGCGTGGTG GTGTCGATGG TAGAACGAAG CGGCGTCGAA GCCTGTAAAG CGGCGGTGCA
      1681 CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC TATCCGCTGG ATGACCAGGA
      1741 TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG TTATTTCTTG ATGTCTCTGA
      1801 CCAGACACCC ATCAACAGTA TTATTTTCTC CCATGAAGAC GGTACGCGAC TGGGCGTGGA
      1861 GCATCTGGTC GCATTGGGTC ACCAGCAAAT CGCGCTGTTA GCGGGCCCAT TAAGTTCTGT
      1921 CTCGGCGCGT CTGCGTCTGG CTGGCTGGCA TAAATATCTC ACTCGCAATC AAATTCAGCC
      1981 GATAGCGGAA CGGGAAGGCG ACTGGAGTGC CATGTCCGGT TTTCAACAAA CCATGCAAAT
      2041 GCTGAATGAG GGCATCGTTC CCACTGCGAT GCTGGTTGCC AACGATCAGA TGGCGCTGGG
      2101 CGCAATGCGC GCCATTACCG AGTCCGGGCT GCGCGTTGGT GCGGATATCT CGGTAGTGGG
      2161 ATACGACGAT ACCGAAGACA GCTCATGTTA TATCCCGCCG TTAACCACCA TCAAACAGGA
      2221 TTTTCGCCTG CTGGGGCAAA CCAGCGTGGA CCGCTTGCTG CAACTCTCTC AGGGCCAGGC
      2281 GGTGAAGGGC AATCAGCTGT TGCCCGTCTC ACTGGTGAAA AGAAAAACCA CCCTGGCGCC
      2341 CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA
      2401 GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTAAGT TAGCTCACTC
      2461 ATTAGGCACC GGGATCTCGA CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC
      2521 GGTGGGCGCG GGGCATGACT ACAGTCACGT AGCGATAGCG GAGTGTATAC TGGCTTAACT
      2581 ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT ATGCGGTGTG AAATACCGCA
      2641 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC
      2701 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG
      2761 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA
      2821 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA
      2881 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
      2941 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
      3001 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
      3061 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
      3121 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
      3181 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
      3241 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC
      3301 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
      3361 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
      3421 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
      3481 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
      3541 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
      3601 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
      3661 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
      3721 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA
      3781 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
      3841 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
      3901 TAATAGTTTG CGCAACGTTG TTGCCATTGC TGCAGGCATC GTGGTGTCAC GCTCGTCGTT
      3961 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
      4021 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
      4081 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
      4141 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
      4201 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
      4261 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
      4321 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
      4381 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
      4441 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AAATTATTGA
      4501 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
      4561 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTAAATT GTAAGCGTTA
      4621 ATGTGAACCA TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG
      4681 GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG
      4741 AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC
      4801 GCTGCGCGTA ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC
      4861 GCCA
//