COMMENT
     pMCSG68         4832 bp
     base vector:    pMCSG53
     BglI-XbaI fragment removed from pMCSG57 and ligated
     into pMCSG53 digested with the same enzymes
     T7 Prom::His6::STREPII::TEV::LIC::T7 Term 
     plus ArgU and IleX tRNAs     
FEATURES             Location/Qualifiers
     tRNA            complement(983..1134)
                     /note="ileX gene"
     rep_origin      complement(2825..3413)
                     /note="pBR322 origin of replication"
     CDS             1437..2396
                     /note="Lac I cds"
     CDS             complement(3584..4444)
                     /note="bla cds"
     tRNA            complement(467..853)
                     /note="argU tRNA"
     misc_feature    complement(250..273)
                     /note="STREP;STREP"
     misc_feature    complement(204..235)
                     /note="LIC Site;LIC Site"
     Cleavage-site   complement(223..243)
                     /note="TEV Site;TEV Site"
     Region          complement(292..309)
                     /note="His Tag;His Tag"
     promoter        complement(384..400)
                     /note="T7 Promoter;T7 Promoter"
     terminator      complement(26..72)
                     /note="T7 Terminator;T7 Terminator"

         1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
        61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
       121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
       181 CGACGGAGCT CGAATTCGGA TCCGTTATCC ACTTCCAATA TTGGATTGGA AGTACAGGTT
       241 CTCGGTACCC TTTTCGAACT GCGGATGAGA CCACAGATCT ACACCAGAAG AATGATGATG
       301 ATGATGGTGC ATATGTATAT CTCCTTCTTA AAGTTAAACA AAATTATTTC TAGAGGGGAA
       361 TTGTTATCCG CTCACAATTC CCCTATAGTG AGTCGTATTA ATTTCGCGGG ATCGAGATCG
       421 ATCTCGATCC TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGTGGATT CAGTCAGGCG
       481 TCCCATTATC AGTGCTTCAG GAAATGGGCG GATGGGAGTC CATAGAAATG GTTCGTAGGT
       541 ATGCTCACCT TGCGCCTAAT CATTTGACAG AGCATGCGAG GAAAATAGAC GACATTTTTG
       601 GTGATAATGT CCCAAATATG TCCCACTCTG GAATTATGGA GGATATAAAG AAGGCGTAAC
       661 TGATTGAATT GTAATGGCGC GCCCTGCAGG ATTCGAACCT GCGGCCCACG ACTTAGAAGG
       721 TCGTTGCTCT ATCCAACTGA GCTAAGGGCG CGTTGATACC GCAATGCGGT GTAATCGCGT
       781 GAATTATACG GTCAACCCTT GCTGAGTCAA TGGCTTTTGA TCTCGCCGGC GCCACAGGTG
       841 CGGTTGCTGG CGCCTATATC GCCGACATCA CCGATGGGGA AGATCGGGCT CGCCACTTCG
       901 GGCTCATGAG CGCTTGTTTC GGCGTGGGTA TGGTGGCAGG CCCCGTGGCC GGGGGACTGT
       961 TGGGCGCCAT CTCCTTGCAT GCAAAAAGGC TGACGATTTC TCGTCAGCCT TTGCTATATC
      1021 TGGTGGCCCC TGCTGGACTT GAACCAGCGA CCAAGCGATT ATGAGTCGCC TGCTCTAACC
      1081 ACTGAGCTAA GGGGCCATGG TAGCGGATTA TAAAGTAACT CCGTGTCGCA ATCCGCATGC
      1141 ACCATTCCTT GCGGCGGCGG TGCTCAACGG CCTCAACCTA CTACTGGGCT GCTTCCTAAT
      1201 GCAGGAGTCG CATAAGGGAG AGCGTCGAGA TCCCGGACAC CATCGAATGG CGCAAAACCT
      1261 TTCGCGGTAT GGCATGATAG CGCCCGGAAG AGAGTCAATT CAGGGTGGTG AATGTGAAAC
      1321 CAGTAACGTT ATACGATGTC GCAGAGTATG CCGGTGTCTC TTATCAGACC GTTTCCCGCG
      1381 TGGTGAACCA GGCCAGCCAC GTTTCTGCGA AAACGCGGGA AAAAGTGGAA GCGGCGATGG
      1441 CGGAGCTGAA TTACATTCCC AACCGCGTGG CACAACAACT GGCGGGCAAA CAGTCGTTGC
      1501 TGATTGGCGT TGCCACCTCC AGTCTGGCCC TGCACGCGCC GTCGCAAATT GTCGCGGCGA
      1561 TTAAATCTCG CGCCGATCAA CTGGGTGCCA GCGTGGTGGT GTCGATGGTA GAACGAAGCG
      1621 GCGTCGAAGC CTGTAAAGCG GCGGTGCACA ATCTTCTCGC GCAACGCGTC AGTGGGCTGA
      1681 TCATTAACTA TCCGCTGGAT GACCAGGATG CCATTGCTGT GGAAGCTGCC TGCACTAATG
      1741 TTCCGGCGTT ATTTCTTGAT GTCTCTGACC AGACACCCAT CAACAGTATT ATTTTCTCCC
      1801 ATGAAGACGG TACGCGACTG GGCGTGGAGC ATCTGGTCGC ATTGGGTCAC CAGCAAATCG
      1861 CGCTGTTAGC GGGCCCATTA AGTTCTGTCT CGGCGCGTCT GCGTCTGGCT GGCTGGCATA
      1921 AATATCTCAC TCGCAATCAA ATTCAGCCGA TAGCGGAACG GGAAGGCGAC TGGAGTGCCA
      1981 TGTCCGGTTT TCAACAAACC ATGCAAATGC TGAATGAGGG CATCGTTCCC ACTGCGATGC
      2041 TGGTTGCCAA CGATCAGATG GCGCTGGGCG CAATGCGCGC CATTACCGAG TCCGGGCTGC
      2101 GCGTTGGTGC GGATATCTCG GTAGTGGGAT ACGACGATAC CGAAGACAGC TCATGTTATA
      2161 TCCCGCCGTT AACCACCATC AAACAGGATT TTCGCCTGCT GGGGCAAACC AGCGTGGACC
      2221 GCTTGCTGCA ACTCTCTCAG GGCCAGGCGG TGAAGGGCAA TCAGCTGTTG CCCGTCTCAC
      2281 TGGTGAAAAG AAAAACCACC CTGGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG
      2341 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC
      2401 AACGCAATTA ATGTAAGTTA GCTCACTCAT TAGGCACCGG GATCTCGACC GATGCCCTTG
      2461 AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG GCATGACTAC AGTCACGTAG
      2521 CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG
      2581 CACCATATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC
      2641 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG
      2701 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA
      2761 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
      2821 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
      2881 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG
      2941 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
      3001 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
      3061 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
      3121 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA
      3181 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
      3241 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG
      3301 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
      3361 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
      3421 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT
      3481 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT
      3541 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA
      3601 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG
      3661 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
      3721 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG
      3781 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC
      3841 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTG
      3901 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC
      3961 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC
      4021 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC
      4081 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT
      4141 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA
      4201 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT
      4261 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
      4321 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA
      4381 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC
      4441 TCATACTCTT CCTTTTTCAA ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG
      4501 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG
      4561 AAAAGTGCCA CCTAAATTGT AAGCGTTAAT GTGAACCATC ACCCTAATCA AGTTTTTTGG
      4621 GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT
      4681 GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG
      4741 CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA
      4801 ATGCGCCGCT ACAGGGCGCG TCCCATTCGC CA
//