FEATURES Location/Qualifiers misc_feature 204..235 /note="LIC Site;LIC Site" Cleavage-site 223..243 /note="TEV Site;TEV Site" Region complement(1387..1404) /note="His Tag;His Tag" CDS complement(205..1407) /note="coding region;coding region" promoter complement(1479..1495) /note="T7 Promoter;T7 Promoter" Region complement(140..157) /note="His Tag;carboxy terminal His tag from pET-21a" terminator complement(26..72) /note="T7 Terminator;T7 Terminator" CDS 2009..2968 /note="Lac I cds;Lac I cds" CDS complement(5157..6017) /note="bla cds;bla cds" rep_origin 4399..4399 /note="pBR322 origin of replication;pBR322 origin of replic ation" Region complement(265..1362) /note="MBP" 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT 121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT 181 CGACGGAGCT CGAATTCGGA TCCGTTATCC ACTTCCAATA TTGGATTGGA AGTACAGGTT 241 CTCGGTACCT GGGATATCGT AATCAGTCTG CGCGTCTTTC AGGGCTTCAT CGACAGTCTG 301 ACGACCGCTG GCGGCGTTGA TCACCGCAGT ACGCACGGCA TACCAGAAAG CGGACATCTG 361 CGGGATGTTC GGCATGATTT CACCTTTCTG GGCGTTTTCC ATGGTGGCGG CAATACGTGG 421 ATCTTTCGCC AACTCTTCCT CGTAAGACTT CAGCGCTACG GCACCCAGCG GTTTGTCTTT 481 ATTAACCGCT TCCAGACCTT CATCAGTCAG CAGATAGTTT TCGAGGAACT CTTTTGCCAG 541 CTCTTTGTTC GGACTGGCGG CGTTAATACC TGCGCTCAGC ACGCCAACGA ACGGTTTGGA 601 TGGTTGACCC TTGAAGGTCG GCAGTACCGT TACACCATAA TTCACTTTGC TGGTGTCGAT 661 GTTGGACCAT GCCCACGGGC CGTTGATGGT CATCGCTGTT TCGCCTTTAT TAAAGGCAGC 721 TTCTGCGATG GAGTAATCGG TGTCTGCATT CATGTGTTTG TTTTTAATCA GGTCAACCAG 781 GAAGGTCAGA CCCGCTTTCG CGCCAGCGTT ATCCACGCCC ACGTCTTTAA TGTCGTACTT 841 GCCGTTTTCA TACTTGAACG CATAACCCCC GTCAGCAGCA ATCAGCGGCC AGGTGAAGTA 901 CGGTTCTTGC AGGTTGAACA TCAGCGCGCT CTTACCTTTC GCTTTCAGTT CTTTATCCAG 961 CGCCGGGATC TCTTCCCAGG TTTTTGGCGG GTTCGGCAGC AGATCTTTGT TATAAATCAG 1021 CGATAACGCT TCAACAGCGA TCGGGTAAGC AATCAGCTTG CCGTTGTAAC GTACGGCATC 1081 CCAGGTAAAC GGATACAGCT TGTCCTGGAA CGCTTTGTCC GGGGTGATTT CAGCCAACAG 1141 GCCAGATTGA GCGTAGCCAC CAAAGCGGTC GTGTGCCCAG AAGATAATGT CAGGGCCATC 1201 GCCAGTTGCC GCAACCTGTG GGAATTTCTC TTCCAGTTTA TCCGGATGCT CAACGGTGAC 1261 TTTAATTCCG GTATCTTTCT CGAATTTCTT ACCGACTTCA GCGAGACCGT TATAGCCTTT 1321 ATCGCCGTTA ATCCAGATTA CCAGTTTACC TTCTTCGATT TTGGTACCCA GATCTACACC 1381 AGAAGAATGA TGATGATGAT GGTGCATATG TATATCTCCT TCTTAAAGTT AAACAAAATT 1441 ATTTCTAGAG GGGAATTGTT ATCCGCTCAC AATTCCCCTA TAGTGAGTCG TATTAATTTC 1501 GCGGGATCGA GATCGATCTC GATCCTCTAC GCCGGACGCA TCGTGGCCGG CATCACCGGC 1561 GCCACAGGTG CGGTTGCTGG CGCCTATATC GCCGACATCA CCGATGGGGA AGATCGGGCT 1621 CGCCACTTCG GGCTCATGAG CGCTTGTTTC GGCGTGGGTA TGGTGGCAGG CCCCGTGGCC 1681 GGGGGACTGT TGGGCGCCAT CTCCTTGCAT GCACCATTCC TTGCGGCGGC GGTGCTCAAC 1741 GGCCTCAACC TACTACTGGG CTGCTTCCTA ATGCAGGAGT CGCATAAGGG AGAGCGTCGA 1801 GATCCCGGAC ACCATCGAAT GGCGCAAAAC CTTTCGCGGT ATGGCATGAT AGCGCCCGGA 1861 AGAGAGTCAA TTCAGGGTGG TGAATGTGAA ACCAGTAACG TTATACGATG TCGCAGAGTA 1921 TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC CAGGCCAGCC ACGTTTCTGC 1981 GAAAACGCGG GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG AATTACATTC CCAACCGCGT 2041 GGCACAACAA CTGGCGGGCA AACAGTCGTT GCTGATTGGC GTTGCCACCT CCAGTCTGGC 2101 CCTGCACGCG CCGTCGCAAA TTGTCGCGGC GATTAAATCT CGCGCCGATC AACTGGGTGC 2161 CAGCGTGGTG GTGTCGATGG TAGAACGAAG CGGCGTCGAA GCCTGTAAAG CGGCGGTGCA 2221 CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC TATCCGCTGG ATGACCAGGA 2281 TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG TTATTTCTTG ATGTCTCTGA 2341 CCAGACACCC ATCAACAGTA TTATTTTCTC CCATGAAGAC GGTACGCGAC TGGGCGTGGA 2401 GCATCTGGTC GCATTGGGTC ACCAGCAAAT CGCGCTGTTA GCGGGCCCAT TAAGTTCTGT 2461 CTCGGCGCGT CTGCGTCTGG CTGGCTGGCA TAAATATCTC ACTCGCAATC AAATTCAGCC 2521 GATAGCGGAA CGGGAAGGCG ACTGGAGTGC CATGTCCGGT TTTCAACAAA CCATGCAAAT 2581 GCTGAATGAG GGCATCGTTC CCACTGCGAT GCTGGTTGCC AACGATCAGA TGGCGCTGGG 2641 CGCAATGCGC GCCATTACCG AGTCCGGGCT GCGCGTTGGT GCGGATATCT CGGTAGTGGG 2701 ATACGACGAT ACCGAAGACA GCTCATGTTA TATCCCGCCG TTAACCACCA TCAAACAGGA 2761 TTTTCGCCTG CTGGGGCAAA CCAGCGTGGA CCGCTTGCTG CAACTCTCTC AGGGCCAGGC 2821 GGTGAAGGGC AATCAGCTGT TGCCCGTCTC ACTGGTGAAA AGAAAAACCA CCCTGGCGCC 2881 CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA 2941 GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTAAGT TAGCTCACTC 3001 ATTAGGCACC GGGATCTCGA CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC 3061 GGTGGGCGCG GGGCATGACT ATCGTCGCCG CACTTATGAC TGTCTTCTTT ATCATGCAAC 3121 TCGTAGGACA GGTGCCGGCA GCGCTCTGGG TCATTTTCGG CGAGGACCGC TTTCGCTGGA 3181 GCGCGACGAT GATCGGCCTG TCGCTTGCGG TATTCGGAAT CTTGCACGCC CTCGCTCAAG 3241 CCTTCGTCAC TGGTCCCGCC ACCAAACGTT TCGGCGAGAA GCAGGCCATT ATCGCCGGCA 3301 TGGCGGCCCC ACGGGTGCGC ATGATCGTGC TCCTGTCGTT GAGGACCCGG CTAGGCTGGC 3361 GGGGTTGCCT TACTGGTTAG CAGAATGAAT CACCGATACG CGAGCGAACG TGAAGCGACT 3421 GCTGCTGCAA AACGTCTGCG ACCTGAGCAA CAACATGAAT GGTCTTCGGT TTCCGTGTTT 3481 CGTAAAGTCT GGAAACGCGG AAGTCAGCGC CCTGCACCAT TATGTTCCGG ATCTGCATCG 3541 CAGGATGCTG CTGGCTACCC TGTGGAACAC CTACATCTGT ATTAACGAAG CGCTGGCATT 3601 GACCCTGAGT GATTTTTCTC TGGTCCCGCC GCATCCATAC CGCCAGTTGT TTACCCTCAC 3661 AACGTTCCAG TAACCGGGCA TGTTCATCAT CAGTAACCCG TATCGTGAGC ATCCTCTCTC 3721 GTTTCATCGG TATCATTACC CCCATGAACA GAAATCCCCC TTACACGGAG GCATCAGTGA 3781 CCAAACAGGA AAAAACCGCC CTTAACATGG CCCGCTTTAT CAGAAGCCAG ACATTAACGC 3841 TTCTGGAGAA ACTCAACGAG CTGGACGCGG ATGAACAGGC AGACATCTGT GAATCGCTTC 3901 ACGACCACGC TGATGAGCTT TACCGCAGCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA 3961 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA 4021 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA 4081 CCCAGTCACG TAGCGATAGC GGAGTGTATA CTGGCTTAAC TATGCGGCAT CAGAGCAGAT 4141 TGTACTGAGA GTGCACCATA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA 4201 TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 4261 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG 4321 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 4381 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 4441 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 4501 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 4561 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG 4621 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 4681 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 4741 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 4801 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 4861 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 4921 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 4981 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 5041 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT 5101 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC 5161 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT 5221 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT 5281 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG 5341 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT 5401 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT 5461 GTTGCCATTG CTGCAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC 5521 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT 5581 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG 5641 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG 5701 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT 5761 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC 5821 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT 5881 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT 5941 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG 6001 AAATGTTGAA TACTCATACT CTTCCTTTTT CAAATTATTG AAGCATTTAT CAGGGTTATT 6061 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 6121 GCACATTTCC CCGAAAAGTG CCACCTAAAT TGTAAGCGTT AATGTGAACC ATCACCCTAA 6181 TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC 6241 CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG 6301 AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA 6361 CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTCCCATT CGCCA //